Cell cycle and organelle specific proteomic analysis of eukaryotic cell

• Project/Area Number: 23770234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: University of Tsukuba
• Principal Investigator: YOSHIDA Masaki 筑波大学, 生命環境系, 助教 (10449308)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: 細胞分裂 / 分裂装置 / 細胞小器官 / プロテオーム / 藻類 / Cyanidioschyzon / ゲノム情報 / オイル産生藻類 / ソフトウェア開発

Outline of Final Research Achievements

We identified novel kinesin-like protein named TOP from unicellular red alga Cyanidioschyzon merolae. It was revealed that TOP composes a dividing machinery of both chloroplast and mitochondrion and regulates organelle division process. In addition, we discovered the third dividing machinery from the peroxisome of C. merolae and clarified the working mechanism of the machinery. We have established a reliable and reproducible method for gene targeting in C. merolae. Our method will be applicable to the manipulation of genes targeted based on proteomic analysis, i.e. gene disruption and replacement. Technology of data analysis method used in the studies above has been patented.

Research Products

• [Journal Article] Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery (2013)
  • Author(s): Yuuta Imoto, Haruko Kuroiwa, Yamato Yoshida, Mio Ohnuma, Takayuki Fujiwara, Masaki Yoshida, Keiji Nishida, Fumi Yagisawa, Shunsuke Hirooka, Shinya Miyagishima, Osami Misumi, Shigeyuki Kawano, Tsuneyoshi Kuroiwa
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 110 Issue: 23 Pages: 9583-9588
  • DOI: 10.1073/pnas.1303483110
  • Peer Reviewed
• [Journal Article] Gene targeting in the red alga Cyanidioschyzon merolae : single- and multi-copy insertion using authentic and chimeric selection markers. (2013)
  • Author(s): Fujiwara T, Ohnuma M, Yoshida M, Kuroiwa T, Hirano T.
  • Journal Title: PLoS One Volume: 8 Issue: 9 Pages: e73608-e73608
  • DOI: 10.1371/journal.pone.0073608
  • Peer Reviewed
• [Journal Article] The kineain-like protein TOP promotes Aurora localisation and induces mitochondrial, chloroplast and nuclear division (2013)
  • Author(s): Yamato Yoshida, Takayuki Fujiwara, Yuuta Imoto, Masaki Yoshida, Mio Ohnuma, Shunsuke Hirooka, Osami Misumi, Haruko Kuroiwa, Tsuneyoshi Kuroiwa
  • Journal Title: Journal of Cell Science Volume: 126 Pages: 2392-2400
  • DOI: 10.1242/jcs.116798
  • Peer Reviewed
• [Journal Article] Single-membrane-bound peroxisome division revealed by isolation of dynamin-based machinery (2013)
  • Author(s): Imoto Y, Kuroiwa H, Yoshida Y, Ohnuma M, Fujiwara T, Yoshida M, Nishida K, Yagisawa F, Hirooka S, Miyagishima S, Misumi O, Kawano S, Kuroiwa T.
  • Journal Title: Proc. Natl. Acad. Sci. Volume: -
  • Peer Reviewed
• [Presentation] コヒーレントアンチストークスラマンによるAurantiochytrium sp. 18W-13aの細胞内脂質解析 (2015)
  • Author(s): 石塚圭、瀬川尋貴、小出昌弘、吉田昌樹、渡邉信、加納英明
  • Organizer: 日本藻類学会第39回福岡大会
  • Place of Presentation: 九州大学 箱崎キャンパス（福岡市）
  • Year and Date: 2015-03-20 – 2015-03-22
• [Presentation] 石油を生むオーランチオキトリウムのコヒーレント・ラマン・イメージング (2014)
  • Author(s): 石塚圭、瀬川尋貴、小出昌弘、吉田昌樹、渡邉信、加納英明
  • Organizer: 第8回分子科学討論会
  • Place of Presentation: 広島大学 東広島キャンパス（東広島市）
  • Year and Date: 2014-09-21 – 2014-09-24
• [Presentation] 海産珪藻Phaeodactylum tricornutum 油滴局在タンパク質に関する研究 (2014)
  • Author(s): 米田 広平・吉田 昌樹・鈴木 石根・渡邉 信
  • Organizer: 藻類学会第37回大会（船橋大会）
  • Place of Presentation: 東邦大学
• [Presentation] Squalene accumulation process of Aurantiochytrium sp. strain NYH1
  • Author(s): Masaki Yoshida
  • Organizer: 1st Korea-Japan Microalgae Symposium (KJMS2013)
  • Place of Presentation: Advanced Institute of Science and Technology, Daejeon, Korea
  • Invited
• [Patent(Industrial Property Rights)] LC-MALDIで得られたデータの比較解析方法 (2011)
  • Inventor(s): 吉田昌樹、黒岩常祥、吉田大和
  • Industrial Property Rights Holder: 吉田昌樹、黒岩常祥、吉田大和
  • Filing Date: 2011
  • Overseas