Elucidation of cell position-dependent Hippo-pathway regulatorymechanisms in preimplantation embryos

• Project/Area Number: 23770265
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: HIRATE Yoshikazu 熊本大学, 発生医学研究所, 助教 (70342839)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞分化 / Hippo 経路 / 細胞接着 / Angiomotin / カドヘリン / カテニン / マウス / 着床前 / Hippo経路 / 着床前胚 / 発生生物学

Research Abstract

Hippo signaling in preimplantation embryos exhibits a cellposition-dependent difference in strength. Inner and outer cells show strong and weaksignaling, respectively. This research clarified the mechanisms of the Hippo pathwayactivation by an adhesion-associated protein Angiomotin and its suppression by cellpolarity. This is the machinery which converts cell position into differential strengthof Hippo signaling. This research uncovered a key problem of the Hippo pathway regulation,which contributes to the progress of the relevant fields

Research Products

• [Journal Article] olarity-dependent distribution ofangiomotin localizes Hippo signaling inpreimplantation embryos. (2013)
  • Author(s): Hirate, Y.
  • Journal Title: Curr. Biol. Volume: Vol.23(in press)
  • Peer Reviewed
• [Journal Article] Tead4 is constitutively nuclear, while nuclear vs. cytoplasmic Yap distribution is regulated in preimplantation embryos. (2012)
  • Author(s): Hirate, Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 109 Issue: 50
  • DOI: 10.1073/pnas.1211810109
  • Peer Reviewed
• [Journal Article] マウス初期胚発生における Hippo pathway の役割 (2011)
  • Author(s): 佐々木洋、平手良和
  • Journal Title: 細胞工学 Volume: Vol. 30 Pages: 935-940
• [Journal Article] マウス初期胚発生におけるHippo pathwayの役割 (2011)
  • Author(s): 佐々木洋、平手良和
  • Journal Title: 細胞工学 Volume: 30 Pages: 935-340
• [Presentation] Cell polarity suppresses Hippo signaling Cell polarity suppresses Hippo signaling (2013)
  • Author(s): Hirate, Y.
  • Organizer: The 46th Annual Meeting of the JapaneseSociety of Developmental Biologists
  • Place of Presentation: くにびきメッセ(島根県松江市)
  • Year and Date: 2013-05-30
• [Presentation] Cell polarity suppresses Hippo signaling through the regulation of Angiomotin distribution in preimplantation mouse embryos (2013)
  • Author(s): Yoshikazu Hirate
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: くにびきメッセ（島根県松江市）
• [Presentation] Position-dependent cell fatespecification by cell polarity-controlledAngiomotin distribution. (2011)
  • Author(s): Hirate. Y.
  • Organizer: Mouse Molecular Genetics 2011
  • Place of Presentation: Hinxton, Cambridge, UK.
  • Year and Date: 2011-09-21
• [Presentation] Position-dependent cell fatespecification by cell polarity-controlledAngiomotin distribution. (2011)
  • Author(s): Hirate, Y.
  • Organizer: KEY Forum in Developmental Biology andRegenerative Medicine
  • Place of Presentation: 熊本大学(熊本).
  • Year and Date: 2011-09-08
• [Presentation] Cell polarity establishes positionaldifference in Hippo signaling by alteringAngiomotin subcellular distribution inpreimplantation mouse embryos (細胞極性による Angiomotin の細胞内分布制御がマウス着床前胚の位置依存的な Hippo 経路活性を確立する). (2011)
  • Author(s): Hirate, Y.
  • Organizer: The 44th Annual Meeting of the JapaneseSociety of Developmental Biologists
  • Place of Presentation: 沖縄コンベンションセンター(沖縄)
  • Year and Date: 2011-05-19
• [Presentation] 細胞極性によるAngiomotinの細胞内分布制御がマウス着床前胚の位置依存的なHippo経路活性を確立する (2011)
  • Author(s): 平手良和
  • Organizer: 日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
• [Presentation] Position-dependent cell fate specification by cell polarity-controlled Angiomotin distribution (2011)
  • Author(s): Yoshikazu Hirate
  • Organizer: Mouse Molecular Genetics 2011
  • Place of Presentation: ウェルカムトラストコンファレンスセンター(英国)