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Functional analysis of OsPtila associated protein complex at the plasma membrane in disease resistance

Research Project

Project/Area Number 23780047
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant pathology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

TAKAHASHI Akira  独立行政法人農業生物資源研究所, 耐病性作物研究開発ユニット, 主任研究員 (20414914)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords植物病理学 / イネ / 病害抵抗性 / 分子遺伝学 / 耐病性
Research Abstract

OsPtila, which is a negative regulator of defense responses in rice, forms a protein complex at plasma membrane. The dynamics of OsPtila-associated protein complex was investigated. As a result, it was indicated that OsPtila functions through protein modification in the complex. Additionally, we identified an Osa protein, which encodes plasma membrane H+-ATPase, as a candidate factor included in the protein complex. Our analysis revealed that the Osa is involved in the early response to elicitor treatment.
OsPti1a, which is a negative regulator of defense responses in rice, forms a protein complex at plasma membrane. The dynamics of OsPti1a-associated protein complex was investigated. As a result, it was indicated that OsPti1a functions through protein modification in the complex. Additionally, we identified an Osa protein, which encodes plasma membrane H+-ATPase, as a candidate factor included in the protein complex. Our analysis revealed that the Osa is involved in the early response to elicitor treatment.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (14 results) (of which Invited: 1 results)

  • [Journal Article] A Receptor-like Cytoplasmic Kinase Targeted by a Plant Pathogen Effector is Directly Phosphorylated by the Chitin Receptor and Mediates Rice Immunity2013

    • Author(s)
      Yamaguchi, K., Yamada, K., Ishikawa, K., Yoshimura, S., Hayashi, N., Uchihashi, K., Ishihama, N., Kishi-Kaboshi, M., Takahashi, A., Tsuge, S., Ochiai, H., Tada, Y., Shimamoto, K., Yoshioka, H., Kawasaki, T.
    • Journal Title

      Cell Host Microbe

      Volume: 13 Pages: 347-357

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The bHLH rac immunityl (RAI1) is activated by OsRac1 via OsMAPK3 and OsMAPK6 in rice immunity2012

    • Author(s)
      Kim, S. H., Oikawa, T., Kyozuka, J., Wong, H. L., Umemura, K., Kishi-Kaboshi, M., Takahashi, A., Kawano, Y., Kawasaki, T., Shimamoto, K.
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Pages: 740-754

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular spectrum of somaclonal variation in regenerated rice revealed by whole-genome sequencing2012

    • Author(s)
      Miyao, A., Nakagome, M., Ohnuma, T., Yamagata, H., Kanamori, H., Katayose, Y., Takahashi, A., Matsumoto, T., Hirochika, H.
    • Journal Title

      Plant Cell Physiol

      Volume: 53 Pages: 256-264

    • NAID

      10030308042

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Down-regulation of UDP-arabinopyranose mutase reduces the proportion of arabinofuranose present in rice cell walls.2011

    • Author(s)
      Konishi, T., Aohara, T., Igasaki, T., Hayashi, N., Miyazaki, Y., Takahashi, A., Hirochika, H., Iwai, H., Satoh, S., Ishii, T.
    • Journal Title

      Phytochemistzy

      Volume: 72 Pages: 1962-1968

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] イネにおける病害応答の発動はサリチル酸の蓄積に依存する2013

    • Author(s)
      高橋章,瀬尾茂美,廣近洋
    • Organizer
      平成25年度日本植物病理学会大会
    • Place of Presentation
      岐阜大学
    • Year and Date
      2013-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] イネMAMPs応答性MYB遺伝子は病害抵抗性反応を増強する2013

    • Author(s)
      加星光子,高橋章,廣近洋彦
    • Organizer
      平成25年度日本植物病理学会大会
    • Place of Presentation
      岐阜大学
    • Year and Date
      2013-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] イネ病害抵抗性におけるサリチル酸の関与2013

    • Author(s)
      高橋章,瀬尾茂美,廣近洋彦
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Year and Date
      2013-03-23
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] イネMAMPs応答性MYB遺伝子は病害抵抗性反応を増強する2013

    • Author(s)
      加星光子,高橋章,廣近洋彦
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学
    • Year and Date
      2013-03-21
    • Related Report
      2012 Final Research Report
  • [Presentation] イネにおける病害応答の発動はサリチル酸の蓄積に依存する2013

    • Author(s)
      高橋章, 瀬尾茂美, 廣近洋彦
    • Organizer
      平成25年度日本植物病理学会大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Transcription factors, which connect MAMPs-responsive MAPK cascade to the coordinately-expressed phenylpropanoid biosynthesis genes2012

    • Author(s)
      Kaboshi M., Takahashi A., Hirochika H.
    • Organizer
      XV international Congress of Molecular plant-Microbe interactions
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2012-07-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Genetic studies of signaling pathways for innateimmunity of rice2012

    • Author(s)
      Takahashi A.
    • Organizer
      Workshop in XV international Congress of Molecular plant-Microbe interactions
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2012-07-29
    • Related Report
      2012 Final Research Report
  • [Presentation] OsPtilaにより制御される病害抵抗性シグナル経路の解明2012

    • Author(s)
      高橋章,松井英譲,瀬尾茂美,伊藤紀仁,来須孝光,朽津和幸,廣近洋彦
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] イネPAMPs応答性MAPKカスケードからフェニルプロパノイド合成系に至るシグナル経路の解析2012

    • Author(s)
      加星光子,高橋章,廣近洋彦
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] イネPAMPs応答性MAPKカスケードからフェニルプロパノイド合成系に至るシグナル経路の解析2012

    • Author(s)
      加星光子,高橋章,廣近洋彦
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大
    • Year and Date
      2012-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] OsPtilaにより制御される病害抵抗性シグナル経路の解明2012

    • Author(s)
      高橋章,松井英譲,瀬尾茂美,廣近洋彦
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Genetic studies of signaling pathways for innate immunity of rice.2012

    • Author(s)
      高橋 章
    • Organizer
      XV international Congress of Molecular Plant-Microbe interactions
    • Place of Presentation
      京都国際会議場
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] OsPti1aにより制御される病害抵抗性シグナル経路の解明2012

    • Author(s)
      高橋 章
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Related Report
      2011 Research-status Report
  • [Presentation] OsPti1aにより制御される病害抵抗性シグナル経路の解明2012

    • Author(s)
      高橋 章
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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