Screening of bifidobacterial genes specifically expressed in the mouse intestine using a site-specific recombination system
| Project/Area Number |
23780072
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
FUKIYA Satoru 北海道大学, 大学院・農学研究院, 助教 (10370157)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ビフィズス菌 / 健康増進効果 / 部位特異的組換え / R-IVET / 特異的遺伝子発現 / Bifidobacterium / 応用微生物 / プロモーター / 遺伝子発現 |
|---|
| Research Abstract |
Bifidobacteria are a major component of the human intestinal microbiota and well-known for their health-promoting effects. However, molecular mechanisms of the effects are still unclear. This study aimed to clarify the mechanisms of the effects by indentifying bifidobacterial genes that are specifically expressed in the intestine. A molecular tool for introduction of the foreign genes to bifidobacterial chromosome was developed. Screening system of the specifically-expressed genes has been constructed based on the developed tool. These progresses are thought to be important for providing evidence at a molecular level for the beneficial effects of bifidobacteria.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation.2012
Author(s)
Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A.
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Journal Title
Appl. Environ. Microbiol
Volume: 78(14)
Pages: 4984-4994
Related Report
Peer Reviewed
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[Journal Article] Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation.2012
Author(s)
Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A.
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Journal Title
Applied and Environmental Microbiology
Volume: 78
Issue: 14
Pages: 4984-4994
DOI
Related Report
Peer Reviewed
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[Journal Article] Technological Advances in Bifidobacterial Molecular Genetics: Application to Functional Genomics and Medical Treatments2012
Author(s)
Fukiya, S., Hirayama, Y., Sakanaka, M., Kano, Y., Yokota, A.
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Journal Title
Bioscience of Microbiota, Food and Health
Volume: 31
Issue: 2
Pages: 15-25
DOI
NAID
ISSN
2186-3342
Related Report
Peer Reviewed
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[Book] In Lactic Acid Bacteria: Current Progress in Advanced Research, Horizon Scientific Press, Norfolk, UK.2011
Author(s)
Fukiya, S., Suzuki, T., Kano, Y., and Yokota, A.
Publisher
Current status of Bifidobacterium gene manipulation technologies.
Related Report
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