Screening of bifidobacterial genes specifically expressed in the mouse intestine using a site-specific recombination system

• Project/Area Number: 23780072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Hokkaido University
• Principal Investigator: FUKIYA Satoru 北海道大学, 大学院・農学研究院, 助教 (10370157)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ビフィズス菌 / 健康増進効果 / 部位特異的組換え / R-IVET / 特異的遺伝子発現 / Bifidobacterium / 応用微生物 / プロモーター / 遺伝子発現

Research Abstract

Bifidobacteria are a major component of the human intestinal microbiota and well-known for their health-promoting effects. However, molecular mechanisms of the effects are still unclear. This study aimed to clarify the mechanisms of the effects by indentifying bifidobacterial genes that are specifically expressed in the intestine. A molecular tool for introduction of the foreign genes to bifidobacterial chromosome was developed. Screening system of the specifically-expressed genes has been constructed based on the developed tool. These progresses are thought to be important for providing evidence at a molecular level for the beneficial effects of bifidobacteria.

Research Products

• [Journal Article] Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. (2012)
  • Author(s): Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A.
  • Journal Title: Appl. Environ. Microbiol Volume: 78(14) Pages: 4984-4994
  • Peer Reviewed
• [Journal Article] Technological advances in bifidobacterial molecular genetics: application to functional genomics and medical treatments. (2012)
  • Author(s): Fukiya, S., Hirayama, Y., Sakanaka, M., Kano, Y., Yokota, A.
  • Journal Title: Biosci. Microb. Food. Health Volume: 31(2) Pages: 15-25
  • NAID: 10030872664
  • Peer Reviewed
• [Journal Article] Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation. (2012)
  • Author(s): Hirayama, Y., Sakanaka, M., Fukuma, H., Murayama, H., Kano, Y., Fukiya, S., Yokota, A.
  • Journal Title: Applied and Environmental Microbiology Volume: 78 Issue: 14 Pages: 4984-4994
  • DOI: 10.1128/aem.00588-12
  • Peer Reviewed
• [Journal Article] Technological Advances in Bifidobacterial Molecular Genetics: Application to Functional Genomics and Medical Treatments (2012)
  • Author(s): Fukiya, S., Hirayama, Y., Sakanaka, M., Kano, Y., Yokota, A.
  • Journal Title: Bioscience of Microbiota, Food and Health Volume: 31 Issue: 2 Pages: 15-25
  • DOI: 10.12938/bmfh.31.15
  • NAID: 130004562662
  • ISSN: 2186-3342
  • Peer Reviewed
• [Presentation] プロバイオティクス機能の分子機構解明に向けたビフィズス菌の遺伝子欠損導入系の開発 (2013)
  • Author(s): 吹谷智,平山洋佑,横田篤
  • Organizer: 大会シンポジウム4SY24「プロバイオティクスの機能評価と新展開-ヒトの健康と疾病予防を支える乳酸菌とビフィズス菌-」日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス(宮城県仙台市)
  • Year and Date: 2013-03-27
• [Presentation] 二重相同組換え法を用いたBifidobacterium longumの染色体上遺伝子への欠失変異導入技術の開発 (2013)
  • Author(s): 吹谷智,平山洋佑,阪中幹祥,福間英訓,村山寛樹,加納康正,横田篤
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学(滋賀県長浜市)
  • Year and Date: 2013-03-10
• [Presentation] 二重相同組換え法を用いたBifidobacterium longumの染色体上遺伝子への欠失変異導入技術の開発 (2013)
  • Author(s): 吹谷 智，平山洋佑，阪中幹祥，福間英訓，村山寛樹，加納康正，横田 篤
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学（滋賀県長浜市）
• [Presentation] プロバイオティクス機能の分子機構解明に向けたビフィズス菌の遺伝子欠損導入系の開発 (2013)
  • Author(s): 吹谷 智，平山 洋佑，横田 篤
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Invited
• [Presentation] ランダム変異導入法への応用を目指したビフィズス菌由来挿入配列TLS143の機能解析 (2012)
  • Author(s): 阪中幹祥、阿部光紗、平山洋佑、吹谷智、加納康正、横田篤
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学(東京都)
  • Year and Date: 2012-03-10
• [Presentation] Transposition activity and insertion property of IS3 family insertion sequence TLS143 isolated from Bifidobacterium longum 105-A (2012)
  • Author(s): Mikiyasu Sakanaka, Arisa Abe, Yosuke Hirayama, Satoru Fukiya, Yasunobu Kano, Atsushi Yokota
  • Organizer: International Conference on Agricultural Biodiversity and Sustainability 2012
  • Place of Presentation: 北海道大学農学部（北海道札幌市）
• [Presentation] ビフィズス菌由来挿入配列TLS143の転移活性の解析 (2012)
  • Author(s): 阪中幹祥，阿部光紗，平山洋佑，吹谷智，加納康正，横田篤
  • Organizer: 第6回日本ゲノム微生物学会若手の会
  • Place of Presentation: ろうきん研修所富士センター（静岡県駿東郡小山町）
• [Presentation] ランダム変異導入法への応用を目指したビフィズス菌由来挿入配列TLS143の機能解析 (2012)
  • Author(s): 阪中幹祥、阿部光紗、平山洋佑、吹谷 智、加納康正、横田 篤
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学（東京都）
• [Presentation] 新規大腸菌-ビフィズス菌シャトルベクターの構築 (2012)
  • Author(s): 小野寺 愛、平山 洋佑、阪中 幹祥、吹谷 智、片山 高嶺、加納 康正、横田 篤
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学（京都市）
• [Presentation] Characterization of transposition property of IS3 family insertion sequence TLS143 isolated from Bifidobacterium longum105-A (2011)
  • Author(s): Satoru Fukiya, Mikiyasu Sakanaka, Arisa Abe, Yosuke Hirayama, Yasunobu Kano, Atsushi Yokota
  • Organizer: 6th Asian Conference on Lactic Acid Bacteria
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 2011-09-08
• [Presentation] Development of targeted gene disruption technique in Bifidobacterium longum105-A (2011)
  • Author(s): Yosuke Hirayama, Hidenori Fukuma, Hiroki Murayama, Satoru Fukiya, Yasunobu Kano, Atsushi Yokota
  • Organizer: 10th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Hotel Zuiderduin(オランダ)
  • Year and Date: 2011-08-28
• [Presentation] Transposition activity and insertion property of IS3 family insertion sequence TLS143 isolated from Bifidobacterium longum 105-A (2011)
  • Author(s): Satoru Fukiya, Mikiyasu Sakanaka, Arisa Abe, Yosuke Hirayama, Yasunobu Kano, Atsushi Yokota
  • Organizer: 10th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Hotel Zuiderduin(オランダ)
  • Year and Date: 2011-08-28
• [Presentation] Bifidobacterium longum 105-A のα-ガラクトシダーゼ遺伝子破壊株の性状解析とレポーターアッセイへの応用 (2011)
  • Author(s): 平山洋佑,平山洋佑、福間英訓、村山寛樹、吹谷智、加納康正、横田篤
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学(大阪府)
  • Year and Date: 2011-07-11
• [Presentation] Bifidobacterium longum 105-Aのα-ガラクトシダーゼ遺伝子破壊株の性状解析とレポーターアッセイへの応用 (2011)
  • Author(s): 平山洋佑、福間英訓、村山寛樹、吹谷 智、加納康正、横田 篤
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学（大阪府）
• [Presentation] Bifidobacterium longum 105-A由来挿入配列TLS143の挿入特異性および転移活性の解析 (2011)
  • Author(s): 阪中幹祥、阿部光紗、平山洋佑、吹谷 智、加納康正、横田 篤
  • Organizer: 日本乳酸菌学会2011年度大会
  • Place of Presentation: 関西大学（大阪府）
• [Presentation] Transposition activity and insertion property of IS3 family insertion sequence TLS143 isolated from Bifidobacterium longum 105-A (2011)
  • Author(s): Satoru Fukiya, Mikiyasu Sakanaka, Arisa Abe, Yosuke Hirayama, Yasunobu Kano, Atsushi Yokota
  • Organizer: 10th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Hotel Zuiderduin（オランダ）
• [Presentation] Development of targeted gene disruption technique in Bifidobacterium longum 105-A (2011)
  • Author(s): Yosuke Hirayama, Hidenori Fukuma ,Hiroki Murayama ,Satoru Fukiya,Yasunobu Kano, Atsushi Yokota
  • Organizer: 10th Symposium on Lactic Acid Bacteria
  • Place of Presentation: Hotel Zuiderduin（オランダ）
• [Presentation] Characterization of transposition property of IS3 family insertion sequence TLS143 isolated from Bifidobacterium longum 105-A (2011)
  • Author(s): Satoru Fukiya, Mikiyasu Sakanaka, Arisa Abe, Yosuke Hirayama, Yasunobu Kano, Atsushi Yokota
  • Organizer: 6th Asian Conference on Lactic Acid Bacteria
  • Place of Presentation: 札幌コンベンションセンター（札幌市）
• [Book] In Lactic Acid Bacteria: Current Progress in Advanced Research, Horizon Scientific Press, Norfolk, UK. (2011)
  • Author(s): Fukiya, S., Suzuki, T., Kano, Y., and Yokota, A.
  • Publisher: Current status of Bifidobacterium gene manipulation technologies.
• [Book] Lactic Acid Bacteria: Current Progress in Advanced Research (2011)
  • Author(s): Satoru Fukiya, Tohru Suzuki, Yasunobu Kano, Atsushi Yokota
  • Publisher: Horizon Scientific Press
• [Remarks]
  • URL: http://www.agr.hokudai.ac.jp/biseibutsu/ja/info.html