Development of one-step protein expression and purification systemUsing silica particles

Research Project

Project/Area Number	23780085
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Applied microbiology
Research Institution	Kyushu University
Principal Investigator	FUJINO Yasuhiro 九州大学, 基幹教育院, 助教 (70582659)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000) Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	シリカ / タンパク質発現 / Thermus / promoter / silica / over-expression
Research Abstract	The aim of this study is to develop novel protein expression and purification system using silica-inducible promoter and silica-binding domain. Transcriptional analysis revealed that iron deficient caused by colloidal silica activated this promoter and ferric uptake regulator (Fur: TTHA0255) regulated this promoter. Almost the same phenomenon was seen in E. coli, and we have succeeded in the construction of silica-inducible expression vector. However, conditions for one-step expression and purification have not completed.

Report

(3 results)