| Project/Area Number |
23780197
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIMASAKI Yohei 九州大学, (連合)農学研究科(研究院), 准教授 (40363329)
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| Co-Investigator(Renkei-kenkyūsha) |
OSHIMA Yuji 九州大学, 大学院農学研究院, 教授 (70176874)
NAKASHIMA Takashi 九州大学, 大学院農学研究院, 助教 (20380553)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | シャトネラ / プロテオミクス / 2-Ｃys Ｐｒｘ / 過酸化水素 / 増殖能力 / 赤潮 / 光合成活性 / peroxidoredoxin |
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| Research Abstract |
Present study aimed at identifying molecular markers reflecting physiological status response to Chattonella marina growth and investigating temporal variation in the potential maximum quantum yield of photosystem II (Fv/Fm ratio) via a field survey and laboratory cultures. Proteome profiles obtained from 2-DE suggested that 2-cysteine peroxiredoxin (2-Cys Prx) play a role in maintaining positive growth of C. marina. The Fv/Fm ratio gradually decreased from approximately 0.72 to 0.62, and the growth rate of C. marina also decreased from approximately 0.5 to <0 div/day during the bloom; there was a significant positive correlation between the two parameters.
Our results suggest that the expression level of 2-Cys Prx and Fv/Fm ratio, combined with information about environmental factors, may be useful in evaluating the growth potential of C. marina during field blooms.
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