A study on search of protein species which contribute to growth of a harmful alga Chattonella

• Project/Area Number: 23780197
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General fisheries
• Research Institution: Kyushu University
• Principal Investigator: SHIMASAKI Yohei 九州大学, (連合)農学研究科(研究院), 准教授 (40363329)
• Co-Investigator(Renkei-kenkyūsha): OSHIMA Yuji 九州大学, 大学院農学研究院, 教授 (70176874) ; NAKASHIMA Takashi 九州大学, 大学院農学研究院, 助教 (20380553)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: シャトネラ / プロテオミクス / 2-Ｃys Ｐｒｘ / 過酸化水素 / 増殖能力 / 赤潮 / 光合成活性 / peroxidoredoxin

Research Abstract

Present study aimed at identifying molecular markers reflecting physiological status response to Chattonella marina growth and investigating temporal variation in the potential maximum quantum yield of photosystem II (Fv/Fm ratio) via a field survey and laboratory cultures. Proteome profiles obtained from 2-DE suggested that 2-cysteine peroxiredoxin (2-Cys Prx) play a role in maintaining positive growth of C. marina. The Fv/Fm ratio gradually decreased from approximately 0.72 to 0.62, and the growth rate of C. marina also decreased from approximately 0.5 to <0 div/day during the bloom; there was a significant positive correlation between the two parameters.
Our results suggest that the expression level of 2-Cys Prx and Fv/Fm ratio, combined with information about environmental factors, may be useful in evaluating the growth potential of C. marina during field blooms.

Research Products

• [Journal Article] Thiobencarb Herbicide Reduces Growth, Photosynthetic Activity, and Amount of Rieske Iron-Sulfur Protein in the Diatom Thalassiosira pseudonana. (2013)
  • Author(s): Shimasaki Y, Tsuyama M, Tasmin R, Qiu X, Shimizu M, Sato Y, Yamasaki Y, Kato-Unoki Y, Nukata A, Nakashima T, Ichinose H, Wariishi H, Honjo T, Oshima Y
  • Journal Title: Journal of biochemical and molecular toxicology Volume: 27 Issue: 9 Pages: 437-444
  • DOI: 10.1002/jbt.21505
  • Peer Reviewed
• [Journal Article] Growth-Phase Dependent Variation in Photosynthetic Activity and Cellular Protein Expression Profile in the Harmful Raphidophyte <i>Chattonella antiqua</i> (2013)
  • Author(s): 邱旭春、島崎洋平.大嶋雄治, 他16名
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 1 Pages: 46-52
  • DOI: 10.1271/bbb.120543
  • NAID: 10031164690
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Low molecular weight allelochemicals produced by the diatom (2012)
  • Author(s): Yamasaki Y, Ohmichi Y, Hirose M, Shikata T, Shimasaki Y, Oshima Y, Honjo T
  • Journal Title: Skeletonema costatum. Thalassas Volume: 28 Pages: 9-17
  • URL: http://webs.uvigo.es/thalassas/thalassas_marco%20principal.htm
• [Journal Article] Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact (2012)
  • Author(s): Qiu X, Yamasaki Y, Shimasaki Y, Gunjikake H, Honda H, Kawaguchi M, Matsubara T, Nagasoe S, Etoh T, Matsui S, Honjo T, Oshima Y
  • Journal Title: Marine Ecology-Progress Series Volume: 446 Pages: 107-118
  • DOI: 10.3354/meps09476
  • Peer Reviewed
• [Journal Article] Growth interactions between the raphidophyte Chattonella antiqua and the dinoflagellate Akashiwo sanguinea (2011)
  • Author(s): Qiu X, Yamasaki Y, Shimasaki Y, Gunjikake H, Matsubara T, Nagasoe S, Etoh T, Matsui S, Honjo T, Oshima Y
  • Journal Title: Harmful Algae Volume: 11 Pages: 81-87
  • DOI: 10.1016/j.hal.2011.08.001
  • Peer Reviewed
• [Journal Article] Allelopathic Effects of Skeletonema spp. May Influence Interspecific Competition and Bloom Formation of Co-occurring Harmful Flagellates
  • Author(s): Qiu X, Shimasaki Y, Yoshida Y, Matsubara T, Yamasaki Y, Kawaguchi M, Honda M, Muori K, Nakajima Y, Tasmin R, Kuno K, Kawamura Y, Honjo T, Oshima Y
  • Journal Title: Journal of the Faculty of Agriculture, Kyushu University Volume: (in press)
  • NAID: 120005472191
• [Presentation] 赤潮海水中におけるシャットネラ細胞の増殖速度、光合成活性および関連タンパク質群の変動 (2014)
  • Author(s): Xuchun Qiu・島崎洋平・松原賢・本城凡夫・大嶋雄治
  • Organizer: 平成26年度日本水産学会春季大会
  • Place of Presentation: 北海道
• [Presentation] 赤潮海水中におけるシャットネラ細胞の増殖速度、光合成活性および関連タンパク質群の変動 (2014)
  • Author(s): Qiu Xuchun ・島崎 洋平 ・松原 賢 ・本城 凡夫 ・大嶋 雄治
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 函館
• [Presentation] 数種植物プランクトン培養株における励起蛍光スペクトルの比較解析 (2014)
  • Author(s): 島崎洋平・本田清一郎・吉田光男・本城凡夫・望岡典隆・沖野望・Xuxhun Qiu・大嶋雄治
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 函館
• [Presentation] ラフィド藻Chattonella marina細胞中の過酸化水素消去酵素peroxidoredoxinの遺伝子解析 (2013)
  • Author(s): 寺本彩乃・島崎洋平・溝口直洋・Qiu Xuchun・鵜木陽子・津山孝人・中島悠・本城凡夫・大嶋雄治
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京)
• [Presentation] 有害ラフィド藻Chattonellaの光合成活性と増殖との関係 (2013)
  • Author(s): Qiu Xuchun・島崎洋平・津山孝人・本田匡人・溝口直洋・松原賢・本城凡夫・大嶋雄治
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] 有害ラフィド藻Chattonellaの光合成活性と増殖との関係 (2013)
  • Author(s): Qiu Xuchun・島崎洋平・津山孝人・本田匡人・溝口直洋・松原賢・本城凡夫・大嶋雄治
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] ラフィド藻 Chattonella marina細胞中の過酸化水素消去酵素peroxidoredoxinの遺伝子解析 (2013)
  • Author(s): 寺本彩乃・島崎洋平・溝口直洋・Qiu Xuchun・鵜木陽子・津山孝人・中島悠・本城凡夫・大嶋雄治
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京
• [Presentation] Effects of herbicide thiobencarb on growth, photosynthetic activity and protein expression of centric diatom Thalassiosira pseudonana (2012)
  • Author(s): Shimasaki Y, Tasmin R, Tsuyama M, Qiu X, Shimizu M, Ichinose H, Kato-Unoki Y, Kang IJ, Honjo T, Oshima Y
  • Organizer: SETAC Asia Pacific
  • Place of Presentation: Kumamoto, Japan
• [Presentation] Temporal variations in photosynthetic activity and phase-dependent cellular protein expression during growth of harmful raphidophyte Chattonella antiqua (2012)
  • Author(s): Qiu X, Shimasaki Y, Tsuyama M, Yamada T, Honda M, Kato-Unoki Y, Nakashima T, Yamasaki Y, Honjo T, Oshima Y
  • Organizer: 15th International Conference on Harmful Algae 2012
  • Place of Presentation: Changwon, Korea
• [Presentation] Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact (2012)
  • Author(s): Shimasaki Y, Qiu X, Yamasaki Y, Honda M, Matsubara T, Honjo T, Oshima Y
  • Organizer: 15th International Conference on Harmful Algae 2012
  • Place of Presentation: Changwon, Korea
• [Presentation] Temporal variations in photosynthetic activity and phase-dependent cellular protein expression during growth of harmful raphidophyte Chattonella antiqua (2012)
  • Author(s): Qiu X, Shimasaki Y, Tsuyama M, Yamada T, Honda M, Kato-Unoki Y, Nakashima T, Yamasaki Y, Honjo T, Oshima Y.
  • Organizer: 15th International Conference on Harmful Algae
  • Place of Presentation: Changwon, Korea
• [Presentation] Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact (2012)
  • Author(s): Shimasaki Y, Qiu X, Yamasaki Y, Honda M, Matsubara T, Honjo T, and Oshima Y.
  • Organizer: 15th International Conference on Harmful Algae
  • Place of Presentation: Changwon, Korea
• [Presentation] Maximum Quantum Yield of Photosystem II and Photosynthesis Related Protein Expression Levels as Possible Bio-indicators of Growth Potential in Harmful Raphidophyte Chattonella antiqua (2011)
  • Author(s): Qiu X, Shimasaki Y, Tsuyama M, Shimizu M, Yamada T, Kuwahara R, Kawaguchi M, Sato Y, Kato-Unoki Y, Nakajima T, Matsubara T, Yamasaki Y, Ichinose H, Wariishi H, Honjo T, Oshima Y
  • Organizer: The 8th International AFAS Joint Symposium between Japan and Korea
  • Place of Presentation: Tottori, Japan
  • Year and Date: 2011-11-16
• [Presentation] ラフィド藻Chattonella marina var. antiquaの増殖に及ぼす強光の影響 (2011)
  • Author(s): 山田拓・島崎洋平・津山孝人・邱旭春・松原賢・本城凡夫・大嶋雄治
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎
• [Presentation] ラフィド藻 Chattonella marina var. antiquaの増殖に及ぼす強光の影響
  • Author(s): 山田拓・島崎洋平・津山孝人・邱旭春・松原賢・本城凡夫・大嶋雄治
  • Organizer: 日本水産学会
  • Place of Presentation: 長崎大学