| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
To provide a basic information for pheromone research in mating of blue crab Callinectes sapidus we tried bimaker targetting in pubertal premolt female which contains sex pheromone. We have found biomarkers in pubertal premolt female urine using 1D and 2D NMR. Amoung the biomarkers, premolt biomarker was purified using various chromatography and identified as N-Acetylglucosamino-1,5-lactone (NAGL). In behavioral and neurophysiological experiment, NAGL was detected by antennule of male which is orfactory receptor organ of the crab. Thus we have found a smell of molting which is a candidate component of sex pheromone mixture using NMR based biomaker targetting.
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