| Project/Area Number |
23780215
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fisheries chemistry
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2013 – 2014
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 渦鞭毛藻 / 増殖 / 競合 / 珪藻 / 分泌タンパク質 / Alexandrium tamarense / 培養上清 |
|---|
| Research Abstract |
We aimed to clarify the molecular mechanisms underlying growth in dinoflagellates Alexandrium tamarense, the causative organism of paralytic shellfish poisoning, in this study. As molecules may participate in the growth, we investigated the proteins secreted by the dinoflagellate. An aliquot of the conditioned medium used for cultivating A. tamarense was investigated, the main component was a 20-kDa protein. Next, we performed cDNA cloning of the gene that encodes the secretory protein. The gene was named as AtCRP. The AtCRP protein secreted in the conditioned medium was identified. Finally, we found that the related species of Chaetoceros didymus inhibited the growth of A. tamarense, and predicted that the molecule participating in this inhibition was existed on the cell membrane.
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