| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Research Abstract |
The purpose of this study is to appear the maternal changes in the blood or uterine that occur in early pregnancy within 10 days after the artificial insemination (AI), to assist the pregnancy recognition at embryo transfer (ET) to improve the conception rate. However, prior to secrete interferon tau from trophectderm, because it was found that changes in gene expression in the uterus of the mother is not observed , it had to be changed the original plan. We, therefore, aimed to explore new pregnancy recognition factors during peri-attachment period. In this study, results were obtained as following: An in vitro coculture system which mimics bovine attachment process was developed. In addition, immortalized cell of uterine epithelial cells was established. Besides, in vitro coculture system appeared EGF secreted into uterine lumen at the initial of attachment process is important. Thus, this in vitro implantation assessment system could be a useful method not only can determine the factors which are important for attachment, but also to explore the improving drugs for pregnancy rate.
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