Study of local immune cell function that regulates corpus luteum development in the cow

• Project/Area Number: 23780288
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Jichi Medical University
• Principal Investigator: SHIRASUNA Koumei 自治医科大学, 医学部, 助教 (20552780)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ウシ / 免疫 / 黄体 / 免疫細胞 / 黄体形成 / 乳牛 / 卵巣 / 免疫機能

Research Abstract

Corpus luteum (CL) formation and regression resembles an inflammatory response due to the influx of immune cells. Neutrophils migrate into the early CL partially due to its major chemoattractant interleukin-8 produced at high levels in the CL, and neutrop

Research Products

• [Journal Article] Emerging roles of immune cells in luteal angiogenesis (2013)
  • Author(s): Shirasuna K, Shimizu T, Motozumi M, Miyamoto A
  • Journal Title: Reproduction Fertility and Development Volume: 25 Issue: 2 Pages: 351-361
  • DOI: 10.1071/rd12096
  • Peer Reviewed
• [Journal Article] Downregulation of lymphatic vessel formation factors in PGF2a-induced luteolysis in the cow (2013)
  • Author(s): Nitta A, Shirasuna K, Nibuno S, Bollwein H, Shimizu T, Miyamoto A
  • Journal Title: Journal of Reproduction and Development Volume: 59 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Upregulation of interferon-stimulated genes and interleukin-10 in peripheral blood immune cells during early pregnancy in dairy cows (2012)
  • Author(s): Shirasuna K, Matsumoto H, Kobayashi E, Nitta A, Haneda S, Matsui M, Kawashima C, Kida K, Shimizu T, Miyamoto A
  • Journal Title: J Reprod Dev Volume: 58 Pages: 84-90
  • NAID: 120006390652
• [Journal Article] Rapid accumulation of polymorphonuclear neutrophils in the corpus luteum during prostaglandin F2α-induced luteolysis in the cow (2012)
  • Author(s): Shirasuna K*, Jiemtaweeboon S, Raddatz S, Nitta A, Schuberth HJ, Bollwein H, Shimizu T, Miyamoto A
  • Journal Title: PLoS ONE Volume: 7 Pages: 29054-29054
  • NAID: 120006732641
• [Journal Article] Possible action of vasohibin-1 as an inhibitor in the regulation of vascularization of the bovine corpus luteum. (2012)
  • Author(s): Shirasuna K*, Kobayashi A, Nitta A, Nibuno S, Sasahara K, Shimizu T, Bollwein H, Miyamoto A
  • Journal Title: Reproduction Volume: 143 Pages: 491-500
• [Journal Article] Expression of prostaglandin F2αreceptor and its isoforms in the bovine corpus luteum during the estrous cycle and PGF2α-induced luteolysis. (2012)
  • Author(s): Shirasuna K, Akabane Y, Beindorff N, Nagai K, Sasaki M, Shimizu T, Bollwein H, Meidan R, Miyamoto A
  • Journal Title: Domest Anim Endocrinol Volume: 43 Pages: 227-238
• [Journal Article] Expression of prostaglandin F2a receptor and its isoforms in the bovine corpus luteum during the estrous cycle and PGF2a-induced luteolysis (2012)
  • Author(s): Shirasuna K, Akabane Y, Beindorff N, Nagai K, Sasaki M, Shimizu T, Bollwein H, Meidan R, Miyamoto A
  • Journal Title: Domestic Animal Endocrinology Volume: 43 Issue: 3 Pages: 227-238
  • DOI: 10.1016/j.domaniend.2012.03.003
  • Peer Reviewed
• [Journal Article] Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow (2011)
  • Author(s): Jiemtaweeboon S, Shirasuna K*, Nitta A, Kobayashi A, Schuberth HJ, Shimizu T, Miyamoto A
  • Journal Title: interleukin-8 in the cow. Volume: 9 Pages: 79-79
• [Journal Article] Possible involvement of IFNT in lymphangiogenesis in the corpus luteum during the maternal recognition period in the cow (2011)
  • Author(s): Nitta A, Shirasuna K*, Haneda S, Matsui M, Shimizu T, Matsuyama S, Kimura K, Schuberth HJ, Miyamoto A.
  • Journal Title: Reproduction Volume: 142 Pages: 879-892
• [Journal Article] Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow (2011)
  • Author(s): Jiemtaweeboon S, Shirasuna K, Nitta A, Kobayashi A, Schuberth HJ, Shimizu T, Miyamoto A.
  • Journal Title: Reproductive Biology and Endocrinology Volume: 9 Issue: 1 Pages: 79-79
  • DOI: 10.1186/1477-7827-9-79
  • Peer Reviewed
• [Presentation] The corpus luteum development regulated by angiogenic factors and polymorphonuclear neutrophils in the cow (2011)
  • Author(s): Shirasuna K, Jiemtaweeboon S, Nitta A, Shimizu T, Miyamoto A.
  • Organizer: JSPS Japan-Poland Joint Seminar
  • Place of Presentation: Iwate Aiina hall (Iwate University), Iwate
• [Presentation] Recruitment of neutrophils within the pregnant corpus luteum in response to interferon tau at the maternal recognition period in the cow (2011)
  • Author(s): Shirasuna K, Matsumoto H, Sinnenard J, Kobayashi A, Haneda S, Matsui M, Shimizu T, Bollwein H, Miyamoto A
  • Organizer: 44th annual meeting; Society for the Study of Reproduction
  • Place of Presentation: Oregon (USA).
• [Presentation] ウシ妊娠認識時期でのインターフェロンτによる黄体内好中球動員機構 (2011)
  • Author(s): 白砂孔明,松本悠,Jiemtaweeboon S,羽田真悟, 松井基純,Bollwein H,清水隆,宮本明夫
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手
• [Presentation] High number of neutrophils exists in the developing corpus lutem and promotes angiogenesis and progesterone secretion in the cow. (2011)
  • Author(s): Sinnenard J, Shirasuna K, Kobayashi A, Schuberth HJ, Shimizu T, Bollwein H, Miyamoto A
  • Organizer: 44th annual meeting; Society for the Study of Reproduction
  • Place of Presentation: Oregon (USA)
• [Presentation] Capacity of the corpus luteum to migrate mononuclear immune cells and their effect on luteal function in the cow (2011)
  • Author(s): Kobayashi A, Shirasuna K, Sinnenard J, Shimizu T, Bollwein H, Miyamoto A
  • Organizer: 44th annual meeting; Society for the Study of Reproduction
  • Place of Presentation: Oregon (USA)
• [Presentation] ウシ妊娠認識時期でのインターフェロンτによる黄体内好中球動員機構 (2011)
  • Author(s): 白砂孔明，松本悠，Jiemtaweeboon S，羽田真悟, 松井基純，Bollwein H，清水隆，宮本明夫
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] The corpus luteum development regulated by angiogenic factors and polymorphonuclear neutrophils in the cow (2011)
  • Author(s): Shirasuna K, Jiemtaweeboon S, Nitta A, Shimizu T, Miyamoto A
  • Organizer: JSPS Japan-Poland Joint Seminar(招待講演)
  • Place of Presentation: 岩手県盛岡市
• [Presentation] Recruitment of neutrophils within the pregnant corpus luteum in response to interferon tau at the maternal recognition period in the cow (2011)
  • Author(s): Shirasuna K, Matsumoto H, Sinnenard J, Kobayashi A, Haneda S, Matsui M, Shimizu T, Bollwein H, Miyamoto A
  • Organizer: 44th annual meeting; Society for the Study of Reproduction
  • Place of Presentation: Oregon (USA)
• [Presentation] High number of neutrophils exists in the developing corpus lutem and promotes angiogenesis and progesterone secretion in the cow (2011)
  • Author(s): Sinnenard J, Shirasuna K, Kobayashi A, Schuberth HJ, Shimizu T, Bollwein H, Miyamoto A
  • Organizer: 44th annual meeting; Society for the Study of Reproduction
  • Place of Presentation: Oregon (USA)
• [Presentation] The possible involvement of interferon tau in lymphangiogenesis in the corpus luteum during maternal recognition period in the cow (2011)
  • Author(s): Nitta A, Shirasuna K, Matsumoto H, Haneda S, Matsui M, Shimizu T, Matsuyama S, Kimura K, Bollwein H, Miyamoto A
  • Organizer: 44th annual meeting; Society for the Study of Reproduction
  • Place of Presentation: Oregon (USA)
• [Presentation] ウシ妊娠認識時におきる黄体内でのリンパ管新生に対するIFNT関与の可能性 (2011)
  • Author(s): 新田あかね，白砂孔明，羽田真悟，松井基純，清水隆，松山秀一, 木村康二，Bollwein H，宮本明夫
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] 腫瘍壊死因子α及びToll-like receptor 4の一塩基多型が乳牛の繁殖性及び免疫機能に与える影響 (2011)
  • Author(s): 川崎友里絵，白砂孔明，青木裕歌，川島千帆，清水隆，宮本明夫
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] ウシ妊娠認識物質インターフェロンτによる免疫制御系システムへの影響 (2011)
  • Author(s): 川村芳輝，白砂孔明，清水隆，松山秀一, 木村康二，宮本明夫
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] 乳牛における成長ホルモン受容体の一塩基多型と繁殖性および泌乳能力との関連性 (2011)
  • Author(s): 宗像めぐみ，白砂孔明，青木裕歌，村山千明，増田豊，宮本明夫，清水隆，木田克弥，川島千帆
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] エストロゲン受容体αの一塩基多型と泌乳持続性が乳牛の繁殖性および乳量へ与える影響 (2011)
  • Author(s): 青木裕歌，白砂孔明，川島千帆，清水隆，村山千明，宗像めぐみ，増田豊，宮本明夫
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] ウシ顆粒層細胞のステロイド産生におけるリポポリサッカライドおよびペプチドグリカンの影響 (2011)
  • Author(s): 清水隆，宮内一樹，白砂孔明，村山千明，宮本明夫
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] ウシ卵胞膜細胞のステロイド産生におけるLipopolysaccharideおよびPeptidoglycanの影響 (2011)
  • Author(s): 堀内まや，村山千明，白砂孔明，宮本明夫，清水隆
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 岩手県盛岡市
• [Presentation] ウシ卵胞膜細胞のステロイド産生における内毒素の影響 (2011)
  • Author(s): 堀内まや，村山千明，白砂孔明，宮本明夫，清水隆
  • Organizer: 第66回北海道畜産学会
  • Place of Presentation: 北海道札幌市
• [Presentation] The predominant infiltration of neutrophils at the very early stage of development into the cyclic bovine corpus luteum (2010)
  • Author(s): Jiemtaweeboon S,白砂孔明,小林明由未, Schuberth HS, 清水隆,宮本明夫
  • Organizer: 第103回日本繁殖生物学会
  • Place of Presentation: 青森