Basic research on establishing of novel diagnosis and therapy using ADAMTS13 in dog with thrombosis.

• Project/Area Number: 23780327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Clinical veterinary science
• Research Institution: Nihon University
• Principal Investigator: MARUYAMA Haruhiko 日本大学, 生物資源科学部, 助教 (60434106)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ADAMTS13 / 犬 / 血栓症

Research Abstract

Human VWF73 is cleaved by canine ADAMTS13. And the decapeptide of the C-terminal edge of human VWF cleaved by canine ADAMTS13 is recognized by the anti-N10 antibody. Therefore the human ADAMTS13 activity ELISA kit, which consists of human VWF73 and anti-N10 antibody, could be applicable for measurement of plasma ADAMTS13 activity in dogs. Furthermore the ADAMTS13 activity in dogs with bacteremia was significantly lower than that in healthy dogs.

Research Products

• [Presentation] ADAMTS13活性測定法に関する研究 (2012)
  • Author(s): 丸山治彦、大竹大賀、金子倫子、亘敏広、鎌田寛、犬
  • Organizer: 第50回日本大学獣医学会
  • Place of Presentation: 日本大学桜門会館、東京
  • Year and Date: 2012-07-01
• [Presentation] Evaluation of a human ELISA kit for the measurement of plasma ADAMTS13 activity in dogs (2012)
  • Author(s): 丸山 治彦、大竹 大賀、金子 倫子、加納 塁、山谷 吉樹、亘 敏広、鎌田 寛
  • Organizer: 2012 American College of Veterinary Internal Medicine Forum
  • Place of Presentation: 米国 ニューオリンズ
• [Presentation] EVALUATION OF A HUMAN ELISA KIT FOR THE MEASUREMENT OF PLASMA ADAMTS13 ACTIVITY IN DOGS. (2012)
  • Author(s): 丸山治彦
  • Organizer: アメリカ獣医内科学会2012
  • Place of Presentation: 米国・ニューオリンズ
• [Remarks] 日本大学生 物資源科学部 獣医学科
  • URL: http://hp.brs.nihon-u.ac.jp/~vethome/