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Analysis of polyamine regulation systems in plants and exploration of functional molecules based on polyamine structure

Research Project

Project/Area Number 23780345
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionTohoku University

Principal Investigator

TAKAHASHI Yoshihiro  東北大学, 大学院・生命科学研究科, 准教授 (20390891)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords代謝工学 / ポリアミン / ポリアミンオキシターゼ / イネ / 病原菌抵抗性反応 / シロイヌナズナ
Research Abstract

Polyamines are important not only for developmental processes but also for various environmental stress responses in plants. In order to elucidate the regulatory system responsible for polyamine homeostasis and action, I focused on polyamine degradationenzyme, polyamine oxidase (PAO), and identified three different genes coding for main PAOs in rice. These proteins were localized to peroxisomes and catalyzed polyamine back conversion. Moreover, it was found that one of the common tetraamines, thermospermine, is involved in disease resistance as a signal molecule like spermine.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (6 results)

  • [Journal Article] Exogenous thermospermine has an activity to induce a subset of the defense genes and restrict cucumber mosaic virus multiplication in Arabidopsis thaliana.2012

    • Author(s)
      Sagor GHM, Takahashi H, Niitsu M, Takahashi Y, Berberich T, Kusano T
    • Journal Title

      Plant Cell Reports

      Volume: (In press) Issue: 7 Pages: 1227-1232

    • DOI

      10.1007/s00299-012-1243-y

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Constitutively and highly expressed Oryza sativa polyamine oxidases localize in peroxisomes and catalyze polyamine back conversion2012

    • Author(s)
      Ono, Y., Kim, D.W., Watanabe, K., Sasaki, A., Niitsu, M., Berberich, T., Kusano, T. and Takahashi, Y
    • Journal Title

      Amino Acids

      Volume: Vol.42 Issue: 2-3 Pages: 867-876

    • DOI

      10.1007/s00726-011-1002-3

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Constitutively and highly expressed Oryza sativa polyamine oxidases localize in peroxisomes and catalyze polyamine back conversion2012

    • Author(s)
      Ono, Y., Kim, D.W., Watanabe, K., Sasaki, A., Niitsu, M., Berberich, T., Kusano, T. and Takahashi, Y.
    • Journal Title

      Amino Acids

      Volume: 42 Pages: 867-876

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Spatio-temporal expression analysis of Arabidopsis thaliana spermine synthase gene promoter2011

    • Author(s)
      Sagor, G.H.M., Yamaguchi, K., Watanabe, K., Berberich, T., Kusano, T. and Takahashi, Y
    • Journal Title

      Plant Biotechnology

      Volume: 28 Issue: 4 Pages: 407-411

    • DOI

      10.5511/plantbiotechnology.11.0704a

    • NAID

      10029707427

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spatio-temporal expression analysis of Arabidopsis thaliana spermine synthase gene promoter2011

    • Author(s)
      Sagor, G.H.M., Yamaguchi, K., Watanabe, K., Berberich, T., Kusano, T. and Takahashi, Y.
    • Journal Title

      Plant Biotechnology

      Volume: 28 Pages: 407-411

    • NAID

      10029707427

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] スペルミンは熱ショック関連遺伝子群の発現を高めることによりシロイヌナズナを熱ショック傷害から保護する2013

    • Author(s)
      Sagor GHM,Berberich Thomas,高橋芳弘,新津勝,草野友延
    • Organizer
      日本ポリアミン学会第4回年会
    • Place of Presentation
      宮城
    • Year and Date
      2013-01-25
    • Related Report
      2012 Final Research Report
  • [Presentation] T-DNA挿入pao変異体植物の外部投与ポリアミンへの反応性に基づくシロイヌナズナのポリアミン酸化酵素の基質特異性への考察2012

    • Author(s)
      土橋隼人,Sagor GHM,新津勝,高橋芳弘,Thomas Berberich,草野友延
    • Organizer
      日本ポリアミン学会第3回年会
    • Place of Presentation
      埼玉
    • Year and Date
      2012-01-26
    • Related Report
      2012 Final Research Report
  • [Presentation] T-DNA挿入pao変異体植物の外部投与ポリアミンへの反応性に基づくシロイヌナズナのポリアミン酸化酵素の基質特異性への考察2012

    • Author(s)
      土橋隼人,Sagor GHM, 新津 勝,高橋芳弘,Thomas Berberich,草野友延
    • Organizer
      日本ポリアミン学会
    • Place of Presentation
      埼玉
    • Related Report
      2011 Research-status Report
  • [Presentation] イネにおけるポリアミン酸化酵素の特徴づけ2011

    • Author(s)
      金東〓,小野裕介,渡辺佳奈子,佐々木彩乃,新津勝,Berberich Thomas,草野友延,高橋芳弘
    • Organizer
      第146回日本農芸化学会東北支部会
    • Place of Presentation
      山形
    • Year and Date
      2011-10-08
    • Related Report
      2012 Final Research Report
  • [Presentation] イネにおけるポリアミン酸化酵素の特徴づけ2011

    • Author(s)
      金東煜,小野裕介,渡辺佳奈子,佐々木彩乃,新津 勝,Berberich Thomas,草野友延,高橋芳弘
    • Organizer
      日本農芸化学会東北支部会
    • Place of Presentation
      山形
    • Related Report
      2011 Research-status Report
  • [Presentation] スペルミンは熱ショック関連遺伝子群の発現を高めることによりシロイヌナズナを熱ショック傷害から保護する

    • Author(s)
      Sagor GHM,Berberich Thomas,高橋芳弘,新津 勝,草野友延.
    • Organizer
      日本ポリアミン学会
    • Place of Presentation
      宮城
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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