Development of analytical method for biological compounds using on-chip liquid chromatography

• Project/Area Number: 23790041
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: TSUNODA Makoto 東京大学, 薬学研究科(研究院), 講師 (10323453)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: マイクロ化学チップ / アミノ酸 / グラジエント溶離 / ミキサー / 分析化学

Research Abstract

High-performance liquid chromatography is an important separation technique used for bioanalysis. In this study, we used pillar array column, which has high separation efficiency. We performed fast and quantitative analysis of branched-chain amino acids in biological samples using a pillar array column and Integration of pillar array columns into a gradient elution system for pressure-driven liquid chromatography.

Research Products

• [Journal Article] Fast and Quantative Analysisi of Branched-Chain Amino Acids in Biological Samples Using a Pillar Array Column (2013)
  • Author(s): Y. Song, K. Takatsuki, M. Isokawa, T. Sekiguchi, J. Mizuno, T. Funatsu, S. Shoji, M. Tsunoda
  • Journal Title: Analytical and Bioanalytical Chemistry Volume: 405 Issue: 25 Pages: 7993-7999
  • DOI: 10.1007/s00216-013-7034-7
  • Peer Reviewed
• [Journal Article] Amino acid analysis using core-shell particle column (2013)
  • Author(s): Y. Song, T. Funatsu, M. Tsunoda
  • Journal Title: Journal of Chromatography B Volume: 927 Pages: 214-217
  • DOI: 10.1016/j.jchromb.2012.09.005
  • Peer Reviewed
• [Journal Article] Integration of pillar array columns into a gradient elution system for pressure-driven liquid chromatography (2012)
  • Author(s): Y. Song, M. Noguchi, K. Takatsuki, T. Sekiguchi, J. Mizuno, T. Funatsu, S. Shoji, M. Tsunoda
  • Journal Title: Anal Chem. Volume: 84( Issue: 11 Pages: 4739-4745
  • DOI: 10.1021/ac3001836
  • Peer Reviewed
• [Journal Article] Amino acids analysis using a monolithic silica column after derivatization with NBD-F (2011)
  • Author(s): Y. Song, T. Funatsu, M. Tsunoda
  • Journal Title: Journal of Chromatography B Volume: 879 Issue: 5-6 Pages: 335-340
  • DOI: 10.1016/j.jchromb.2010.12.018
  • Peer Reviewed
• [Presentation] ピラーアレイカラムを用いたヒト血漿中分岐鎖アミノ酸の定量分析 (2013)
  • Author(s): 磯川宗生、宋彦延、高月克也、関口哲志、水野潤、船津高志、庄子習一、角田誠
  • Organizer: 第3回新アミノ酸分析研究会
  • Place of Presentation: 東京都文京区
  • Year and Date: 2013-12-02
• [Presentation] 高分解能オンチップ液体クロマトグラフィーの開発 (2013)
  • Author(s): 磯川宗生、青山千顕、野口真男、水野潤、庄子習一、船津高志、角田誠
  • Organizer: 第24回クロマトグラフィー科学会議
  • Place of Presentation: 東京都文京区
  • Year and Date: 2013-11-12
• [Presentation] Quantitative determination of branched-chain amino acids in human plasma using on-chip chromatography with pillar array (2013)
  • Author(s): Y. Song, K. Takatsuki, M. Isokawa, T. Sekiguchi, J. Mizuno, T. Funatsu, S. Shoji, M. Tsunoda
  • Organizer: RSC (Royal Society of Chemistry) Tokyo International Conference
  • Place of Presentation: 千葉県千葉市
  • Year and Date: 2013-09-05
• [Presentation] オンチップ液体クロマトグラフィーによる高性能分離媒体の開発 (2013)
  • Author(s): 角田誠、青山千顕、野口真男、水野潤、庄子習一、船津高志
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2013-03-28
• [Presentation] Gradient elution on-chip chromatography (2013)
  • Author(s): M. Tsunoda
  • Organizer: Pittcon 2013
  • Place of Presentation: Philadelphia USA
  • Year and Date: 2013-03-19
• [Presentation] Pillar Array Columns with Low Dispersion Turns for Pressure-Driven Reversed-Phase Liquid Chromatography (2012)
  • Author(s): M. Tsunoda et al
  • Organizer: Pittcon2012
  • Place of Presentation: Orland USA
  • Year and Date: 2012-03-13
• [Presentation] Pillar Array Columns with Low Dispersion Turns for Pressure-Driven Reversed-Phase Liquid Chromatography (2012)
  • Author(s): Makoto Tsunoda et al.
  • Organizer: Pittcon2012
  • Place of Presentation: Orland, USA
• [Presentation] Pillar Array Column with Low Dispersion Turns for Pressure-Driven Liquid Chromatography (2011)
  • Author(s): M. Tsunoda, et al.
  • Organizer: 37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: 中国・大連
  • Year and Date: 2011-10-09
• [Presentation] Pillar Array Column with Low Dispersion Turns for Pressure-Driven Liquid Chromatography (2011)
  • Author(s): Makoto Tsunoda et al.
  • Organizer: 37th International Symposium on High Performance Liquid Phase Separations and Related Techniques
  • Place of Presentation: 中国、大連
• [Presentation] Gradient elution on-chip chromatography
  • Author(s): Makoto Tsunoda
  • Organizer: Pittcon2013
  • Place of Presentation: Philadelphia, USA
• [Presentation] オンチップ液体クロマトグラフィーによる高性能分離媒体の開発
  • Author(s): 角田 誠、青山千顕、野口真男、水野潤、庄子習一、船津高志
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜