| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Heparanase is an enzyme which involved in cancer cell invasion and metastasis. In this research, the purpose is to clarify the 3D-structure of heparanase.
I examined various protein expression systems using E. coli and silk worm, and succeeded in obtaining about 5 mg of the target protein from the 1L culture as a soluble fraction. The antibody was produced using the prepared recombinant protein as an antigen, and I confirmed that the antibody specifically recognized heparanase by ELISA. Then, I tried to crystallize complexes of heparanase with produced antibody, and succeeded in obtaining some micro crystals. Moreover, I also succeeded in obtaining the single crystals of beta-glucuronidase, which belongs to heparanase type enzyme.
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