Cardiac regeneration therapy with human mesenchymal stem cells activated with angiotensin receptor blockers

• Project/Area Number: 23790197
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Keio University
• Principal Investigator: NUMASAWA Yohei 慶應義塾大学, 医学部, 共同研究員 (60414059)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 間葉系幹細胞 / アンギオテンシン

Research Abstract

Human bone marrow-derived mesenchymal stem cell might not be a good cardiac stem cell source, because of its low cardiomyogenic transdifferentiation efficiency. We hypothesized that angiotensin receptor blockers might improve cardiomyogenic transdifferentiation efficiency of mesenchymal stem cell because they have protective effects for many human organs including heart. Pretreatment of mesenchymal stem cells with angiotensin receptor blockers significantly increased the cardiomyogenic transdifferentiation efficiency in vitro and in vivo. The pretreatment of mesenchymal stem cells with angiotensin receptor blockers improved cardiomyogenic transdifferentiation efficiency, and might increase the efficacy of mesenchymal stem cell transplantation in cardiac stem cell therapy.

Research Products

• [Journal Article] Successful percutaneous coronary intervention of a chronic totally occluded left anterior descending artery via an ipsilateral intraseptal channel using intravascular ultrasound-guided retrograde wire-crossing technique. (2014)
  • Author(s): Numasawa Y, Motoda H, Yamazaki H, Kuno T, Takahashi T.
  • Journal Title: Cardiovascular Intervention and Therapeutics Volume: 29 Issue: 2 Pages: 157-161
  • DOI: 10.1007/s12928-013-0204-x
  • Peer Reviewed
• [Journal Article] Successful percutaneous thrombectomy in an elderly patient with massive pulmonary embolism with cardiogenic shock. (2014)
  • Author(s): Numasawa Y, Motoda H, Yamazaki H, Kuno T, Hashimoto O, Takahashi T.
  • Journal Title: Cardiovascular Intervention and Therapeutics Volume: 29 Issue: 1 Pages: 70-75
  • DOI: 10.1007/s12928-013-0194-8
  • Peer Reviewed
• [Journal Article] Successful Percutaneous Coronary Intervention of Chronic Total Occlusion of the Right Coronary Artery Using “Bidirectional Kissing-Balloon Technique” (2014)
  • Author(s): Numasawa Y, Hamazaki Y, Takahashi T.
  • Journal Title: Journal of Invasive Cardiology Volume: 未定
  • Peer Reviewed
• [Journal Article] Successful Percutaneous Coil Embolization of a Large and Tortuous Coronary Artery to Pulmonary Artery Fistula (2013)
  • Author(s): Numasawa Y, Motoda H, Yamazaki H, Kuno T, Takahashi T.
  • Journal Title: Internal Medicine Volume: 52 Issue: 23 Pages: 2623-2627
  • DOI: 10.2169/internalmedicine.52.1076
  • NAID: 130003383724
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Impact of coronary dominance on in-hospital outcomes after percutaneous coronary intervention in patients with acute coronary syndrome. (2013)
  • Author(s): Kuno T, Numasawa Y, Miyata H, Takahashi T, Sueyoshi K, Ohki T, Negishi K, Kawamura A, Kohsaka S, Fukuda K.
  • Journal Title: PLos One Volume: 8 Issue: 8 Pages: e72672-e72672
  • DOI: 10.1371/journal.pone.0072672
  • Peer Reviewed
• [Journal Article] A rare adult case of left main coronary artery atresia. (2013)
  • Author(s): Numasawa Y, Motoda H, Yamazaki H, Kuno T, Hashimoto O, Takahashi T.
  • Journal Title: Cardiovascular Intervention and Therapeutics Volume: 28 Issue: 3 Pages: 313-317
  • DOI: 10.1007/s12928-013-0168-x
  • Peer Reviewed
• [Journal Article] Anatomical variations affect radial artery spasm and procedural achievement of transradial cardiac catheterization. (2013)
  • Author(s): Numasawa Y, Kawamura A, Kohsaka S, Takahashi M, Endo A, Arai T, Ohno Y, Yuasa S, Maekawa Y, Fukuda K.
  • Journal Title: Heart Vessels Volume: 未定 Issue: 1 Pages: 49-57
  • DOI: 10.1007/s00380-013-0324-3
  • Peer Reviewed
• [Journal Article] Use of Thrombolysis in Myocardial Infarction Risk Score to predict bleeding complications in patients with unstable angina and non-ST elevation myocardial infarction undergoing percutaneous coronary intervention. (2013)
  • Author(s): Numasawa Y, Kohsaka S, Miyata H, Kawamura A, Noma S, Suzuki M, Nakagawa S, Momiyama Y, Takahashi T, Sato Y, Fukuda K.
  • Journal Title: Cardiovascular Intervention and Therapeutics Volume: 未定 Issue: 3 Pages: 242-249
  • DOI: 10.1007/s12928-013-0162-3
  • NAID: 40019716771
  • Peer Reviewed
• [Journal Article] Safety of transradial approach for percutaneous coronary intervention in relation to body mass index: a report from a Japanese multicenter registry. (2013)
  • Author(s): Numasawa Y, Kohsaka S, Miyata H, Kawamura A, Noma S, Suzuki M, Nakagawa S, Momiyama Y, Sato Y, Fukuda K.
  • Journal Title: Cardiovascular Intervention and Therapeutics Volume: 28 Issue: 2 Pages: 148-156
  • DOI: 10.1007/s12928-012-0138-8
  • NAID: 40019634873
  • Peer Reviewed
• [Journal Article] 心不全の疫学 (2013)
  • Author(s): 沼澤洋平、香坂俊
  • Journal Title: HEART nursing Volume: 26 Pages: 10-11
• [Journal Article] Treatment of human mesenchymal stem cells with angiotensin receptor blocker improved efficiency of cardiomyogenic transdifferentiation and improved cardiac function via angiogenesis (2011)
  • Author(s): Numasawa Y, Kimura T, Miyoshi S, Nishiyama N, Hida N, Tsuji H, Tsuruta H, Segawa K, Ogawa S, Umezawa A
  • Journal Title: Stem Cells Volume: 29(9) Issue: 9 Pages: 1405-14
  • DOI: 10.1002/stem.691
  • Peer Reviewed
• [Journal Article] Successful percutaneous coil embolization of coronary-pulmonary, -carotid, and -in ternal mammary artery fistulas (2011)
  • Author(s): Numasawa Y, Kawamura A, Hashimoto S, Endo A, Yuasa S, Maekawa Y, Kuribayashi S, Fukuda K
  • Journal Title: Heart Vessels Volume: (In press) Issue: 3 Pages: 331-336
  • DOI: 10.1007/s00380-011-0172-y
  • Peer Reviewed
• [Journal Article] Treatment of Human Mesenchymal Stem Cells with Angiotensin Receptor Blocker Improved Efficiency of Cardiomyogenic Transdifferentiation and Improved Cardiac Function via Angiogenesis (2011)
  • Author(s): Yohei Numasawa, Takehiro Kimura, Shunichiro Miyoshi, Nobuhiro Nishiyama, Naoko Hida, Hiroko Tsuji, Hikaru Tsuruta, Kaoru Segawa, Satoshi Ogawa, Akihiro Umezawa
  • Journal Title: Stem Cells Volume: 29 Pages: 1405-1414
  • Peer Reviewed
• [Presentation] Incidence and Predictors of Bleeding Complications after Percutaneous Coronary Intervention: Comparison of Model Performance with Patients’ Characteristics and Technical Factors (2014)
  • Author(s): Yohei Numasawa
  • Organizer: 第78回日本循環器学会総会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] Unique “Obesity Paradox” in Japanese Patients with Coronary Artery Disease: a Report from a Multicenter Percutaneous Coronary Intervention Registry (2014)
  • Author(s): Yohei Numasawa
  • Organizer: 第78回日本循環器学会総会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] Successful Percutaneous Treatment of a Large Saphenous Vein Graft Aneurysm Using Covered Stents (2013)
  • Author(s): Yohei Numasawa
  • Organizer: 第22回日本心血管インターベンション治療学会
  • Place of Presentation: 神戸国際展示場
• [Presentation] New PPI Guide Wire Paddler & GLAIVE (2013)
  • Author(s): Yohei Numasawa
  • Organizer: Tokyo Percutaneous cardiovascular Intervention Conference
  • Place of Presentation: セルリアンタワー東急ホテル
  • Invited
• [Presentation] Impact of Sex on In-hospital Clinical Outcomes among Patients Undergoing Percutaneous Coronary Intervention in the Japanese Real-World Multicenter Registry (2013)
  • Author(s): Yohei Numasawa
  • Organizer: 第77回日本循環器学会総会
  • Place of Presentation: パシフィコ横浜 （神奈川県）
• [Presentation] Angiotensin Receptor Blockers Improved Cardiomyogenic Transdifferentiation Efficacy of Human Marrow-derived Mesenchymal Stem Cells in Vitro (2011)
  • Author(s): Yohei Numasawa, Shunichiro Miyoshi, Takehiro Kimura, Nobuhiro Nishiyama, Hiroko Tsuji, Naoko Hida, Daisuke Shinmura, Hikaru Tsuruta, Kaoru Segawa, Yuiko Tsukada, Makoto Handa, Akihiro Umezawa, Satoshi Ogawa
  • Organizer: 第75回日本循環器学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-08-04
• [Presentation] Angiotensin Receptor Blockers Improved Cardiomyogenic Transdifferentiation Efficacy of Human Marrow-derived Mesenchymal Stem Cells in Vitro (2011)
  • Author(s): Yohei Numasawa
  • Organizer: 第75回日本循環器学会総会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] 心不全を契機に診断された先天性左冠動脈閉鎖症の1成人例
  • Author(s): 沼澤洋平
  • Organizer: 第589回日本内科学会関東地方会
  • Place of Presentation: 日本都市センター