Development of shield-arms typed oligonucleotides for cancer therapy

• Project/Area Number: 23790202
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: FUJIWARA Naruyoshi 聖マリアンナ医科大学, 医学部, 助教 (50365425)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 医療薬剤学 / ドラッグデリバリーシステム / ナノドラッグ / 遺伝子製剤 / デコイ型遺伝子 / IRF-1 / デコイ型遺伝子製剤 / リポソーム

Research Abstract

In this study, I showed that the shield-arms type oligonucleotide was able to down-regulate VEGF expression dependent on TGF-〓 in macrophage and induce anti-tumor effect by enhancement of IRF-1 expression in macrophage residing the tumor environment. It is suggested that this oligonucleotide will become new strategy of anti-angiogenesis by suppression of cancer as shields and activation for macrophages asarms.

Research Products

• [Journal Article] Establishment of retinal progenitor cell clones by transfection with Pax6 gene of mouse induced pluripotent stem (iPS) cells. (2012)
  • Author(s): Suzuki N, et. al.
  • Journal Title: Neurochem Res. Volume: 37 Pages: 835-45
  • Peer Reviewed
• [Journal Article] Role of SDF1/CXCR4 Interaction in Experimental Hemiplegic Models with Neural Cell Transplantation. (2012)
  • Author(s): Arimitsu N, et. al.
  • Journal Title: Int J Mol Sci. Volume: 13 Pages: 2636-49
  • Peer Reviewed
• [Journal Article] Excessive CD4+ T cells co-expressing interleukin-17 and interferon-γ in patients with Behçet's disease. (2011)
  • Author(s): Shimizu J, et. al.
  • Journal Title: Clin Exp Immunol. Volume: 168 Pages: 68-74
  • Peer Reviewed
• [Presentation] 癌治療を目的としたシールドアームズ型遺伝子製剤の開発 (2012)
  • Author(s): 藤原成芳
  • Organizer: 日本薬学会第132 年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-31
• [Presentation] Effect of shield-arms typed oligonucleotides on regulation of tumor-associated macrophage function. (2012)
  • Author(s): Fujiwara N, et, al.
  • Organizer: 日本薬学会
  • Place of Presentation: 札幌（北海道大学）
• [Patent(Industrial Property Rights)] Method of delivering agent into target cell (2011)
  • Inventor(s): HAGA, Makoto; (JP).FUJIWARA, Naruyoshi; (JP).NISHI, Akihiro; (JP).KUBO, Satoru; (JP).KUSHIBIKI, Takanori; (JP)
  • Industrial Property Rights Holder: HAGA, Makoto [JP/JP]; (JP) (For US Only).FUJIWARA, Naruyoshi [JP/JP]; (JP) (For US Only).NISHI, Akihiro [JP/JP]; (JP) (For US Only).KUBO, Satoru [JP/JP]; (JP) (For US Only).KUSHIBIKI, Takanori[JP/JP]; (JP) (For US Only)
  • Filing Date: 2011
  • Overseas