The role of FGF signaling in genital development: for understanding the mechanisms underlying hypospadias.

• Project/Area Number: 23790229
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: HARADA Masayo 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (80555756)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 外生殖器 / FGF / 尿道 / 尿道下裂

Research Abstract

The goal of this research is to understand the developmental mechanisms of external genitalia in which congenital malformations such as hypospadias occur at a high frequency. The roles of FGF signaling during the external genitalia outgrowth and urethral tubulogenesis were investigated using genetically modified mice. This research demonstrated that 1)mesenchymal FGF signaling is required for the GT outgrowth, 2)Ectodermal FGF signaling is required for urethral tube formation,3)Endodermal FGF signaling contributes to stratification of urethral epithelium. These findings will contribute to the understanding of the molecular mechanisms of hypospadias.

Research Products

• [Journal Article] Epithelial Bmp (Bone morphogenetic protein) signaling for bulbourethral gland development : a mouse model for congenital cystic dilation (2011)
  • Author(s): Omori A, Harada M, Ohta S, Villacorte M, Sugimura Y, Shiraishi T, Suzuki K, Nakagata N, Ito T, Yamada G
  • Journal Title: Congenital Anomalies (Kyoto) Volume: 51 Issue: 3 Pages: 102-109
  • DOI: 10.1111/j.1741-4520.2011.00318.x
  • Peer Reviewed
• [Presentation] FGF9 monomer-dimer equilibrium regulates extracellular matrix affinity and tissue diffusion to mediate joint development (2012)
  • Author(s): Harada M, Murakami H, Okawa A, Okimoto N, Hiraoka S, Nakahara T, Akasaka R, Siraishi Y, Futatsugi N, Mizutani-Koseki Y, Kuroiwa A, Shirouzu M, Yokoyama S, Taiji M, Iseki S, Ornitz DM, Koseki H, Nimura A, Yamaguchi K, Akita K.
  • Organizer: Hong Kong Society for Developmental Biology Symposium From Embryology to Disease Mechanisms
  • Place of Presentation: Hong Kong
• [Presentation] FGF9 monomer-dimer equilibrium regulates extracellular matrix affinity and tissue diffusion to mediate joint development (2011)
  • Author(s): Harada M, Murakami H, Okawara A, Okimoto N, Hiraoka S, Nakahara T, Akasaka R, Shiraishi Y, Futatsugi N, Kuroiwa A, Yokoyma S, Taiji M, Iseki S, Ornitz DM, Yamaguchi K, Akita K, Koseki H.
  • Organizer: Molecular Genetics
  • Place of Presentation: Hinxton, Cambridge, UK
• [Presentation] FGF9 monomer-dimer equilibrium regulates extracellular matrix affinity and tissue diffusion to mediate joint development (2011)
  • Author(s): Harada M, Murakami H, Okawar A, Okimoto N, Hiraoka S, Nakahara T, Akasaka R, Shiraishi Y, Futatsugi N, Kuroiwa A, Yokoyma S, Taiji M, Iseki S, Ornitz DM, Yamaguchi K, Akita K, Koseki H
  • Organizer: Molecular Genetics 20th - 23rd Sep 2011
  • Place of Presentation: Wellcome Trust Conference Centre, Hinxton, Cambridge, UK
• [Book] 論文ができてしまう! (2011)
  • Author(s): 原田理代、山田源
  • Total Pages: 477
  • Publisher: 中山書店
• [Book] 論文ができてしまう！疾患モデルマウス表現型解析指南 ３章疾患モデルマウス：臓器・疾患別解析・実験法 泌尿器生殖器系 (2011)
  • Author(s): 原田理代、山田源 （編集 山村研一、若菜茂晴）
  • Total Pages: 477
  • Publisher: 中山書店