The effect of HA4 against hypoxic-ischemic brain injury

• Project/Area Number: 23790239
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Juntendo University
• Principal Investigator: SUNABORI Takehiko 順天堂大学, 医学部, 助教 (00407115)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 低酸素 / 脳虚血 / 神経細胞死 / ヒアルロン酸4糖 / Toll 様受容体 / 炎症性サイトカイン / 海馬 / Toll様受容体 / ヒアルロン酸４糖

Research Abstract

I found that hyaluronan tetrasaccharide (HA4)-treated mice rescued the hippocampal pyramidal neurons 24hours after H-I injury, and that the protective effect of neurons by HA4 reduced expression of inflammatory cytokines related to the inactivation of NF・B.Both in vivo and in vitro data suggested that the reduced expression of inflammatory cytokines was due to the agonistic effect of HA4 against the Toll-like receptor 2.

Research Products

• [Journal Article] Purinergic signaling promotes proliferation of adult mouse subventricular zone cells (2012)
  • Author(s): Suyama S, Sunabori T, Kanki H, Sawamoto K, Gachet C, Koizumi S, Okano H.
  • Journal Title: J Neurosci Volume: 32巻27号 Pages: 9238-9247
• [Journal Article] Purinergic signaling promotes proliferation of adult mouse subventricular zone cells. (2012)
  • Author(s): Suyama S, et al
  • Journal Title: Journal of Neuroscience Volume: 32(27) Issue: 27 Pages: 9238-9247
  • DOI: 10.1523/jneurosci.4001-11.2012
  • Peer Reviewed
• [Journal Article] Fbxw7-dependent degradation of Notch is required for control of stemness and neuronal-glial differentiation in neural stem cells (2011)
  • Author(s): Matsumoto A, Onoyama I, Sunabori T, Kageyama R, Okano H, Nakayama KI
  • Journal Title: J Biol Chem Volume: 286巻15号 Pages: 13754-13764
• [Journal Article] Purified mesenchymal stem cells are an efficient source for iPS cell induction. (2011)
  • Author(s): Niibe K, Kawamura Y, Araki D, Morikawa S, Miura K, Suzuki S, Shimmura S, Sunabori T, Mabuchi Y, Nagai Y, Nakagawa T, Okano H, Matsuzaki Y.
  • Journal Title: PLOS One Volume: 6巻3号
• [Journal Article] Fbxw7-dependent degradation of Notch is required for control of syemness and neuronal-glial differentiation in neural stem cells (2011)
  • Author(s): Matsumoto, A., Onoyama, I., Sunabori, T., Kageyama, R., Okano, H., and Nakayama, K. I.
  • Journal Title: J. Biol. Chem Volume: 286 Issue: 15 Pages: 13754-13764
  • DOI: 10.1074/jbc.m110.194936
  • Peer Reviewed
• [Journal Article] Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction (2011)
  • Author(s): Niibe K
  • Journal Title: PLoS One Volume: 6 Issue: 3 Pages: e17610-e17610
  • DOI: 10.1371/journal.pone.0017610
  • Peer Reviewed
• [Presentation] Toll様受容体を介したオートファジー誘導機構について (2013)
  • Author(s): 砂堀毅彦
  • Organizer: 第118回日本解剖学会総会
  • Place of Presentation: 高松
• [Presentation] Inactivation of Toll-like receptors 2/4 by hyaluronan tetrasaccharide suppresses hypoxic-ischemic injury in hippocampal pyramidal neurons. (2012)
  • Author(s): Sunabori T
  • Organizer: Hershey Conference
  • Place of Presentation: London
• [Presentation] ヒアルロン酸4糖は低酸素/脳虚血負荷依存性にToll様受容体2/4の活性化を抑制することで海馬錐体細胞に神経保護効果を示す (2012)
  • Author(s): 砂堀毅彦
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Protective effects of hyaluronan tetrasaccharide on hippocampal pyramidal neurons in neonatal mouse brains after hypoxic-ischemic injury
  • Author(s): 砂堀毅彦
  • Organizer: 第３４回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Protective effects of hyaluronan tetrasaccharide on hippocampal pyramidal neurons in neonatal mouse brains after hypoxic-ischemic injury
  • Author(s): Takehiko Sunabori
  • Organizer: 第４２回北米神経科学学会 Neuro2011
  • Place of Presentation: Washington DC, USA
• [Presentation] Protective effects of hyaluronan tetrasaccharide on hippocampal pyramidal neurons in neonatal mouse brains after hypoxic-ischemic injury
  • Author(s): 砂堀毅彦
  • Organizer: 第１１７回日本解剖学会総会
  • Place of Presentation: 山梨大学
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/shinkei_kozo/index.html
• [Patent(Industrial Property Rights)] オートファジー性細胞死抑制剤 (2011)
  • Inventor(s): 内山安男、小池正人、砂堀毅彦、大貫洋二、三池神也
  • Industrial Property Rights Holder: 内山安男、小池正人、砂堀毅彦、大貫洋二、三池神也
  • Industrial Property Number: 2011-054647
  • Filing Date: 2011-03-11