Molecular mechanism of mineral transport related to cellular reactive oxygen species level
| Project/Area Number |
23790264
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 活性酸素 / ミネラル / トランスポーター / X線吸収スペクトル / タンパク質結晶構造解析 / Ｘ線吸収スペクトル |
|---|
| Research Abstract |
A fine-tuned balance between production and scavenging of reactive oxygen species (ROS) is crucial for living cells. Since minerals (essential trace elements) can be a factorin regulation of ROS level, mechanisms of mineral transport at molecular level are essential for understanding the tuning mechanism of the ROS level in living cells. In this study, techniques for analysis of local structure around mineral using X-ray absorption spectroscopy has been established. Also, micro crystals of selenoprotein P, a selenium transporter, have been obtained. Further crystallographic study will be contributed the finding of scavenging mechanism of ROS by selenium-sites in the protein.
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Report
(3 results)
Research Products
(3 results)
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[Journal Article] Creation of a Binuclear Purple Copper Site within a de Novo Coiled-Coil Protein2012
Author(s)
D. Shiga, Y. Funahashi, H. Masuda, A. Kikuchi, M. Noda, S. Uchiyama, K. Fukui, K. Kanaori, K. Tajima, Y. Takano, H. Nakamura, M. Kamei, T. Tanaka
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Journal Title
Biochemistry
Volume: 51
Issue: 40
Pages: 7901-7907
DOI
Related Report
Peer Reviewed
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