Role of T-cell protein tyrosine phosphatase (TCPTP) in the hypothalamus controlling feeding behavior

• Project/Area Number: 23790276
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: FUKUSHIMA Atsushi 聖マリアンナ医科大学, 医学部, 助教 (10442716)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: チロシン脱リン酸化酵素 / TCPTP / NPY / POMC / 視床下部 / チロシン脱リン酸化酵素脱リン酸化酵素 / ARC / STAT3 / エストロジェン

Research Abstract

The purpose of the present study was to show the possible involvement of T-cell protein tyrosine phosphatase（TCPTP） via STAT3 in pro-opiomelanocortin（POMC） neurons in feeding behavior. To this end, we tried to use Cre-Lox recombination to generate POMC neurons specific deletion of TCPTP. However, we came to realize that the mouse strain which TCPTP was selectively knocked out from POMC neurons showed no behavioral abnormality andexhibited normal feeding behavior and glucose torelance. Thus we thought that another hypothalamic neuron was involved and NPY neuron was candidate. We decided to use NPY-mVenus mouse for the analysis. We also examined the regulation of the TCPTP expression by estrogen in chow and high fat fed mice. We found that the TCPTP expression was up regulated by estrogen and high fat diet. We are currently examining these results using NPY-mVenus mouse.

Research Products

• [Presentation] (2013)
  • Author(s): 福島篤、他2名
  • Organizer: 第90回日本生理学会
  • Place of Presentation: 東京都
  • Year and Date: 2013-03-30
• [Presentation] 視床下部のTCPTPを介したエストロゲンによる摂食抑制の機序 (2013)
  • Author(s): 福島 篤、舩橋 利也、明間 立雄
  • Organizer: 第90回日本生理学会
  • Place of Presentation: 東京都 江戸川区
• [Presentation] (2012)
  • Author(s): 福島篤、他3名
  • Organizer: 第39回日本神経内分泌学会
  • Place of Presentation: 福岡県
  • Year and Date: 2012-09-28
• [Presentation] (2012)
  • Author(s): 福島篤、他3名
  • Organizer: 第89回日本生理学会
  • Place of Presentation: 長野県
  • Year and Date: 2012-03-29
• [Presentation] 視床下部のTCPTP発現と摂食行動調節の役割 (2012)
  • Author(s): 福島 篤、由利 和也、舩橋 利也、明間 立雄
  • Organizer: 第39回日本神経内分泌学会
  • Place of Presentation: 福岡県 北九州市
• [Presentation] T-cell protein tyrosine phosphatase in peripheral and brain tissues is newcomet to controlling metabolism.
  • Author(s): 福島篤、他3名
  • Organizer: 第89回 日本生理学会
  • Place of Presentation: 長野県松本市 (信州大学)