Functional analysis of the hi stone methyltransferase SETDB1
| Project/Area Number |
23790364
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | 独立行政法人国立循環器病研究センター (2012)
Osaka University (2011) |
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Principal Investigator |
TACHIBANA Keisuke 独立行政法人国立循環器病研究センター, 研究所, 室長 (30432446)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 代謝異常学 / SETDB1 / ヒストンメチル化酵素 / 相互作用解析 / 翻訳後修飾 |
|---|
| Research Abstract |
To analyze the function of SETDB1, we generated plasmids to express SETDB1 proteins of various sizes. The plasmids were expressed in Sf9 cells and purified. A histone methyltransferase assay using purified SETDB1 revealed that SETDB1 requires not only the SET domain, but also the N-terminal region of the SET domain for full enzymatic activity in vitro. We also identified that the SET domain of SETDB1 was phosphorylated. In addition, immunoprecipitation experiments revealed that SETDB1 was associated with MCAF1.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Human mannose-binding lectin 2 is directly regulated by peroxisome proliferator-activated receptors (PPARs) via a peroxisome proliferator responsive element2013
Author(s)
Tachibana K, Takeuchi K, Inada H, Sugimoto K, Ishimoto K, Yamashita M, Maegawa T, Yamasaki D, Osada S, Tanaka T, Rakug H, Hamakubo T, Sakai J, Kodama T, Doi T
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Journal Title
The Journal of Biochemistry
Volume: 154 (3)
Issue: 3
Pages: 265-73
DOI
Related Report
Peer Reviewed
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[Journal Article] Fenofibrate suppresses growth of the human hepatocellular carcinoma cell via PPARa-independent mechanisms2011
Author(s)
D. Yamasaki, N. Kawabe, H. Nakamura, K. Tachibana, K. Ishimoto, T. Tanaka, H. Aburatani, J. Sakai, T. Hamakubo, T. Kodama, T. Doi
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Journal Title
Eur J Cell Biol
Volume: 90
Pages: 657-664
Related Report
Peer Reviewed
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[Presentation] ヒト核内受容体PPARa(こよる生体防御レクチンMBL2の発現制御機構の解析2012
Author(s)
橘敬祐, 竹内健太郎, 稲田大彦, 杉本研, 石本憲司, 田中十志也, 楽木宏実, 浜窪隆雄, 酒井寿郎, 児玉龍彦, 沢村達也, 土井健史
Organizer
第121回日本薬理学会近畿部会
Place of Presentation
徳島
Year and Date
2012-06-29
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[Presentation] 核内レセプターPPARdeltaの翻訳後修飾と相互作用因子による転写制御機構の解明2011
Author(s)
橘敬祐, 上原光太郎, 前川貴志, 熊谷文子, 藤田泰聖, 樋野展正, 山下雅礼, 秋山恵麻, 谷本恵一, 石本憲司, 山崎大典, 川村猛, 田中十志也, 酒井寿郎, 浜窪隆雄, 児玉龍彦, 土井健史
Organizer
日光シンポジウム
Place of Presentation
栃木
Year and Date
2011-12-17
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[Presentation] 転写制御因子を介したミトコンドリアの脂肪酸酸化制御の解析2011
Author(s)
山下雅礼, 橘敬祐, 杉本研, 秋山恵麻, 石本憲司, 山崎大典, 岩成宏子, 田中十志也, 望月康弘,楽木宏実, 酒井寿郎, 浜窪隆雄, 児玉龍彦, 土井健史
Organizer
第34回日本分子生物学会年会
Place of Presentation
横浜
Year and Date
2011-12-14
Related Report
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[Presentation] ヒストンメチル化酵素SETDB1の細胞内局在の解析2011
Author(s)
後藤英子, 橘敬祐, 内原佳恵, 川又那津子, 石本憲司,岩成宏子, 川村猛, 望月康弘, 酒井寿郎, 浜窪隆雄, 児玉龍彦, 土井健史
Organizer
第84回日本生化学会大会
Place of Presentation
京都
Year and Date
2011-09-24
Related Report
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[Presentation] ヒストンメチル化酵素SETDB1はメチル基供与体SAMの輸送体になり得るか?2011
Author(s)
橘敬祐, 後藤英子, 内原佳恵, 川又那津子, 石本憲司, 山崎大典, 岩成宏子, 望月康弘, 酒井寿郎, 浜窪隆雄, 児玉龍彦, 土井健史
Organizer
第6回トランスポーター研究会
Place of Presentation
仙台
Year and Date
2011-06-11
Related Report
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