| Project/Area Number |
23790364
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | 独立行政法人国立循環器病研究センター (2012)
Osaka University (2011) |
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Principal Investigator |
TACHIBANA Keisuke 独立行政法人国立循環器病研究センター, 研究所, 室長 (30432446)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 代謝異常学 / SETDB1 / ヒストンメチル化酵素 / 相互作用解析 / 翻訳後修飾 |
|---|
| Research Abstract |
To analyze the function of SETDB1, we generated plasmids to express SETDB1 proteins of various sizes. The plasmids were expressed in Sf9 cells and purified. A histone methyltransferase assay using purified SETDB1 revealed that SETDB1 requires not only the SET domain, but also the N-terminal region of the SET domain for full enzymatic activity in vitro. We also identified that the SET domain of SETDB1 was phosphorylated. In addition, immunoprecipitation experiments revealed that SETDB1 was associated with MCAF1.
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