Experimental study of cellular damage and carcinogenesis of hepatocyte

• Project/Area Number: 23790449
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Nagoya City University
• Principal Investigator: NAIKI Aya 名古屋市立大学, 大学院・医学研究科, 助教 (20509236)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ギャップ結合 / 肝発がん / 動物モデル

Research Abstract

Connexin 32 (Cx32) is a major gap junction protein in the liver. We established Cx32 dominant negative transgenic rats and Cx32 transfected rat hepatocellular carcinoma cells. The present study by using them indicated that caspase 3 dependent apoptosis was an important mechanism of acetaminophen-induced hepatotoxicity. Moreover, Cx32 regulates apoptotic signaling in both normal liver tissue and hepatocellular carcinoma cells, may play important roles in preventing carcinogenesis by inducing apoptosis in genetically damaged cells with cancer initiation.

Research Products

• [Journal Article] Purple corn color inhibition of prostate carcinogenesis by targeting cell growth pathways. (2013)
  • Author(s): Long N, Suzuki S, Sato S, Naiki-Ito A, Sakatani K, Shirai T, Takahashi S
  • Journal Title: Cancer Sci Volume: 104 Issue: 3 Pages: 298-303
  • DOI: 10.1111/cas.12078
  • Peer Reviewed
• [Journal Article] Age dependent carcinogenic susceptibility in rat liver is related to potential of gap junctional intercellular communication. (2012)
  • Author(s): Naiki-Ito A, Kato H, Asamoto M, Naiki T, Shirai T
  • Journal Title: Toxicol. Pathol. Volume: 40(5) Issue: 5 Pages: 715-721
  • DOI: 10.1177/0192623312441402
  • Peer Reviewed
• [Journal Article] Silencing of connexin 43 suppresses invasion, migration and lung metastasis of rat hepatocellular carcinoma cells. (2012)
  • Author(s): Ogawa K, Pitchakarn P, Suzuki S, Chewonarin T, Tang M, Takahashi S, Naiki-Ito A, Sato S, Takahashi S, Asamoto M, Shirai T.
  • Journal Title: Cancer Sci. Volume: 103(5) Issue: 5 Pages: 860-867
  • DOI: 10.1111/j.1349-7006.2012.02228.x
  • Peer Reviewed
• [Journal Article] Organ specific Gst-pi expression of the metastatic androgen independent prostate cancer cells in nude mice (2012)
  • Author(s): Naiki T, Asamoto M, Toyoda-Hokaiwado N, Naiki-Ito A, Tozawa K, Kohri K, Takahashi S, Shirai T
  • Journal Title: Prostate Volume: 72 Issue: 5 Pages: 533-41
  • DOI: 10.1002/pros.21455
  • Peer Reviewed
• [Journal Article] TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2 (2012)
  • Author(s): Delhase M, Kim SY, Lee H, Naiki-Ito A, Chen Y, Ahn ER, Murata K, Kim SJ, Lautsch N, Kobayashi KS, Shirai T, Karin M, Nakanishi M
  • Journal Title: Proc Natl Acad Sci Volume: 109(4) Issue: 4 Pages: 177-86
  • DOI: 10.1073/pnas.1119296109
  • Peer Reviewed
• [Journal Article] Silencing of connexin 43 suppresses invasion, migration and lung metastasis of rat hepatocellularcarcinoma cells (2012)
  • Author(s): Ogawa K, Pitchakarn P, Suzuki S, Chewonarin T,Tang M, Takahashi S, Naiki-Ito A, Sato S, Takahashi S, Asamoto M, Shirai T
  • Journal Title: Cancer Sci Volume: 103 Issue: 2 Pages: 860-7
  • DOI: 10.1111/j.1365-2559.2011.04018.x
  • Peer Reviewed
• [Presentation] Histone deacetylase inhibition has potential to suppresse growth of androgen dependent and independent prostate cancer in vitroand in vivo (2013)
  • Author(s): Shinya Sato, Shugo Suzuki, Aya Naiki-Ito, Yoriko Yamashita, Makoto Asamoto, Tomoyuki Shirai, Satoru Takahashi
  • Organizer: AACR Annual Meeting 2013
  • Place of Presentation: 米国、ワシントン
  • Year and Date: 2013-04-07
• [Presentation] ラット肝癌細胞における Acetaminophen 誘導アポトーシスに対する細胞間コミュニケーションの関与 (2013)
  • Author(s): 内木綾、朝元 誠人、加藤 寛之、白井智之、高橋 智
  • Organizer: 第29回日本毒性病理学会学術集会
  • Place of Presentation: 茨城県
  • Year and Date: 2013-01-31
• [Presentation] ラット肝癌細胞におけるAcetaminophen 誘導アポトーシスに対する細胞間コミュニケーションの関与 (2013)
  • Author(s): 内木 綾、朝元 誠人、加藤 寛之、白井 智之、高橋 智
  • Organizer: 第29回日本毒性病理学会学術集会
  • Place of Presentation: オークラフロンティアホテルつくば（茨城県）
• [Presentation] ラット肝癌細胞でのアポトーシス誘導におけるコネキシン 32 の役割 (2012)
  • Author(s): 春日部 こずえ、内木綾、朝元 誠人、白井 智之、高橋 智
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道
  • Year and Date: 2012-09-21
• [Presentation] Ethanol promotes diethylnitrosamine-induced hepatocarcinogenesis in connexin32 dominant negative transgenic rats (2012)
  • Author(s): 内木綾、朝元 誠人、加藤 寛之、白井智之、高橋 智
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道
  • Year and Date: 2012-09-19
• [Presentation] Age dependent carcinogenic susceptibility in rat liver is related to potential of gap junctional intercellular communication (2012)
  • Author(s): Naiki-ito A, Asamoto A, Takahashi S, Shirai T
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: 米国、シカゴ
  • Year and Date: 2012-04-04
• [Presentation] Organ-specific Gst-pi expression in new metastatic androgen-independent prostate cancer animal model (2012)
  • Author(s): Taku Naiki, Makoto Asamoto, Aya Naiki-Ito, Kenji Yamada, Ryosuke Ando, Youichi Ikegami, Noriyasu Kawai, Keiichi Tozawa, Satoru Takahashi, Kenjiro Kohri
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: 米国、シカゴ
  • Year and Date: 2012-04-03
• [Presentation] ギャップ結合タンパクconnexin32 によるラット肝発がんと細胞死制御. (2012)
  • Author(s): 内木綾
  • Organizer: 第28回日本毒性病理学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2012-02-02
• [Presentation] Age dependent carcinogenic susceptibility of rat liver is related to potential of gap junctional intercellular communication (2012)
  • Author(s): Aya Naiki-Ito, Makoto Asamoto, Satoru Takahashi, Tomoyuki Shirai
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: McCormick Place（アメリカ、シカゴ）
• [Presentation] ラット肝癌細胞でのアポトーシス誘導におけるコネキシン３２の役割 (2012)
  • Author(s): 春日部 こずえ、内木 綾、朝元 誠人、白井智之、高橋 智
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（北海道）
• [Presentation] Ethanol promotes diethylnitrosamine-induced hepatocarcinogenesis in connexin32 dominant negative transgenic rats (2012)
  • Author(s): 内木 綾、加藤 寛之、朝元 誠人、白井 智之、高橋 智
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（北海道）
• [Presentation] 加齢ラットの肝発がん感受性は、Cx32による細胞間連絡能に制御される (2012)
  • Author(s): 内木 綾、朝元 誠人、佐藤 慎哉、鈴木 周五、高橋 智
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 京王プラザホテル（東京都）
• [Presentation] ギャップ結合タンパクconnexin 32によるラット肝発がんと細胞死制御 (2012)
  • Author(s): 内木 綾
  • Organizer: 第28回日本毒性病理学会学術集会(招待講演)
  • Place of Presentation: 一橋記念講堂（東京都）
• [Presentation] The role of connexin32 formed gap junctional intercellular communication in hepatocarcinogenesis (2011)
  • Author(s): 内木綾、朝元 誠人、鈴木 周五、高橋智、白井 智之
  • Organizer: 70回日本癌学会学術総会
  • Place of Presentation: 愛知県)
  • Year and Date: 2011-10-03
• [Presentation] ギャップ結合タンパクconnexin 32によるラット肝発がんとアポトーシス制御 (2011)
  • Author(s): 内木 綾、朝元 誠人、鈴木 周五、高橋 智、白井 智之
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県）