Analysis of shiga-toxin resistance systemin enterohemorrhagic E. coli

• Project/Area Number: 23790478
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Miyazaki
• Principal Investigator: OGURA Yoshitoshi 宮崎大学, フロンティア科学実験総合センター, 助教 (40363585)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 病原性 / 腸管出血性大腸菌 / 志賀毒素

Research Abstract

Major virulence factor of enterohemorrhagic E. coliis shiga toxin (Stx) which inhibits protein synthesis of the host cell by depurinating a specific adenine residue of 28S rRNA. The E. coli K-12 also exhibits growth defect by introduction and expression of Stx. However O157 strain is resistant to Stx. In this study, from the analysis of O157 fosmid clones which confer Stx resistance to K-12, three genomic regions of O157 genome related to the Stx resistance were identified.

Research Products

• [Journal Article] Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis (2013)
  • Author(s): Nagayasu E, Ogura Y, Itoh T, Yoshida A, Chakraborty G, Hayashi T, Maruyama H
  • Journal Title: Parasitology International Volume: 62(1) Issue: 1 Pages: 57-65
  • DOI: 10.1016/j.parint.2012.09.006
  • Peer Reviewed
• [Journal Article] A human outbreak case associated with Escherichia albertii (2013)
  • Author(s): T. Ooka, E. Tokuoka, M. Fukuhara, T. Nagamura, Y. Ogura, K. Aisawa, S. Harada, T. Hayashi
  • Journal Title: Emerg. Infect. Dis. Volume: 19 Issue: 1 Pages: 144-146
  • DOI: 10.3201/eid1901.120646
  • URL: http://dx.doi.org/
  • Peer Reviewed
• [Journal Article] Complete genome sequence of a Propionibacterium acnes isolate from a sarcoidosis patient. (2013)
  • Author(s): Minegishi K, Aikawa C, Furukawa A, Watanabe T, Nakano T, Ogura Y, Ohtsubo Y, Kurokawa K, Hayashi T, Maruyama F, Nakagawa I, Eishi Y
  • Journal Title: Genome Announcement Volume: 1 Issue: 1 Pages: 16-12
  • DOI: 10.1128/genomea.00016-12
  • Peer Reviewed
• [Journal Article] Complete Genome Sequence of a Propionibacterium acnes Isolate from a Sarcoidosis Patient (2013)
  • Author(s): K. Minegishi, C. Aikawa, A. Furukawa, T. Watanabe, T. Nakano, Y. Ogura, Y. Ohtsubo, K. Kurokawa, T. Hayashi, F. Maruyama, I. Nakagawa, Y. Eishi
  • Journal Title: Genome Announc. Volume: 1 Pages: 1-1
  • Peer Reviewed
• [Journal Article] Detection of diverse aquatic microbes in blood and organs of drowning victims : First metagenomic approach using high-throughput 454-pyrosequencing (2012)
  • Author(s): Kakizaki E, et al
  • Journal Title: Forensic Science International Volume: (in press) Issue: 1-3 Pages: 135-146
  • DOI: 10.1016/j.forsciint.2012.02.010
  • Peer Reviewed
• [Journal Article] A sensitive and simple plaque formation method for the Stx2 phage of Escherichia coliO157:H7, which does not form plaques in the standard plating procedure (2012)
  • Author(s): Md. R. Islam, Y. Ogura, Md. Asdulghani, T. Ooka, K. Murase, Y. Gotoh, T. Hayashi
  • Journal Title: Plasmid Volume: 67(3) Pages: 227-235
  • Peer Reviewed
• [Journal Article] Complete genome sequence of Helicobacter cinaedi strain PAGU611, isolated in a case of human bacteremia (2012)
  • Author(s): Goto T, et al
  • Journal Title: J Bacteriol Volume: 194 Issue: 14 Pages: 3744-3745
  • DOI: 10.1128/jb.00645-12
  • Peer Reviewed
• [Journal Article] Hemolysin E- and enterohemolysin-derived hemolytic activity of O55/O157 strains and other Escherichia coli lineages (2012)
  • Author(s): K.Murase, et al
  • Journal Title: Microbiology Volume: 158 Issue: 3 Pages: 746-758
  • DOI: 10.1099/mic.0.054775-0
  • Peer Reviewed
• [Journal Article] Clinical significance of Escherichia albertii (2012)
  • Author(s): T. Ooka, K. Seto, K. Kawano, H. Kobayashi, Y. Etoh, S. Ichihara, A. Kaneko, J. Isobe, K. Yamaguchi, K. Horikawa, T.A.T. Gomes, A. Linden, M. Bardiau, J. Mainil, L. Beutin, Y. Ogura, T. Hayashi
  • Journal Title: Emerg. Infect. Dis Volume: 18(3) Pages: 488-492
  • Peer Reviewed
• [Journal Article] Is bile acid a determinant of the gut microbiota on a high-fat diet? (2012)
  • Author(s): A. Yokota, S. Fukiya, T. Ooka, Y. Ogura, T. Hayashi, S. Ishizuka
  • Journal Title: Gut Microbes Volume: 3(5) Issue: 5 Pages: 455-459
  • DOI: 10.4161/gmic.21216
  • URL: http://dx.doi.org/
  • Peer Reviewed
• [Journal Article] Whole-Genome Sequence of the Hypervirulent Clinical Strain Mycobacterium intracellulare M.i.198. (2012)
  • Author(s): Tateishi, Y., S. Kitada, K. Miki, R. Maekura, Y. Ogura, Y. Ozeki, Y. Nishiuchi, M. Niki, T. Hayashi, K. Hirata, K. Kobayashi, and S. Matsumoto.
  • Journal Title: Journal of Bacteriology Volume: 194 Issue: 22 Pages: 6336-6336
  • DOI: 10.1128/jb.01439-12
  • Peer Reviewed
• [Journal Article] 腸管出血性大腸菌O111の進化と病原性 (2012)
  • Author(s): 小椋義俊, 大岡唯祐, 林哲也
  • Journal Title: 神経内科 Volume: 76(2) Pages: 155-163
• [Journal Article] 腸管出血性大腸菌における病原性のゲノム進化 (2012)
  • Author(s): 小椋義俊, 林哲也
  • Journal Title: 感染・炎症・免疫 Volume: 42 Pages: 182-195
• [Journal Article] 腸管出血性大腸菌O111の進化と病原性 (2012)
  • Author(s): 小椋義俊, 大岡唯祐, 林哲也
  • Journal Title: 神経内科 Volume: 76 Pages: 155-163
• [Journal Article] Bile Acid is a Host Factor that Regulates the Composition of the Cecal Microbiota in Rats (2011)
  • Author(s): Islam KBMS, Fukiya S, Hagio M, Fujii N, Ishizuka S, Ooka T, Ogura Y, Hayashi T, Yokota A
  • Journal Title: Gastroenterology Volume: VOL. 141 Issue: 5 Pages: 1773-1781
  • DOI: 10.1053/j.gastro.2011.07.046
  • Peer Reviewed
• [Journal Article] Wide distribution of O157-antigen biosynthesis gene clusters 1 in Escherichia coli (2011)
  • Author(s): A.Iguchi, et al
  • Journal Title: PLoS ONE Volume: 6 Issue: 8 Pages: e23250-e23250
  • DOI: 10.1371/journal.pone.0023250
  • Peer Reviewed
• [Journal Article] The Lifestyle of the Segmented Filamentous Bacterium : A Non-Culturable Gut-Associated Immunostimulating Microbe Inferred by Whole-Genome Sequencing (2011)
  • Author(s): Kuwahara T, et al
  • Journal Title: DNA Research Volume: 18 Issue: 4 Pages: 291-303
  • DOI: 10.1093/dnares/dsr022
  • Peer Reviewed
• [Journal Article] Characterisation of the Porphyromonas gingivalis conjugative transposon CTnPg1 : determination of the integration site and the genes essential for conjugal transfer (2011)
  • Author(s): M.Naito, et al
  • Journal Title: Microbiology Volume: 157 Issue: 7 Pages: 2022-2032
  • DOI: 10.1099/mic.0.047803-0
  • Peer Reviewed
• [Journal Article] IS621-based multiplex PCR printing method of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle (2011)
  • Author(s): J.G.Mainil, et al
  • Journal Title: J.Appl.Microbiol. Volume: 111 Issue: 3 Pages: 773-786
  • DOI: 10.1111/j.1365-2672.2011.05089.x
  • Peer Reviewed
• [Journal Article] The genome of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas, reveals new insights into the evolution of Firmicutes and the organism's intracellular adaptations (2011)
  • Author(s): Y.Ogawa, et al
  • Journal Title: J.Bacteriol. Volume: 193 Issue: 12 Pages: 2959-2971
  • DOI: 10.1128/jb.01500-10
  • Peer Reviewed
• [Journal Article] Genome sequence of photobacterium mandapamensis strain svers. 1.1, the bioluminescent symbiont of the cardinal fish siphamia versicolor (2011)
  • Author(s): H.Urbanczyk, et al
  • Journal Title: J.Bacteriol. Volume: 193 Issue: 12 Pages: 3144-3145
  • DOI: 10.1128/jb.00370-11
  • Peer Reviewed
• [Presentation] 高解像度系統解析による腸管出血性大腸菌O157のStx2高産生性系統の同定 (2013)
  • Author(s): 小椋義俊, Md Rakibul Islam, 真子俊博, 大岡唯祐, 大西真, 林哲也
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] 腸管出血性大腸菌O26におけるゲノムアダプテーション解析 (2013)
  • Author(s): 小椋義俊, 桂啓介, 伊藤武彦, Mainil Jacques , 吉野修司, 磯部順子, 勢戸和子, 江藤良樹, 黒木真理子, 木全恵子, 前田詠里子, 後藤恭宏, 大岡唯祐, 林哲也
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜
• [Presentation] O157 Stx2 phageの包括的ゲノム比較・機能解析により明らかとなったStx2高産生型phagenの存在 (2012)
  • Author(s): 小椋義俊, Md. Rakibul Islam, 真子俊博, 大岡唯祐, 大西真, 林哲也
  • Organizer: 第16回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 秋田
• [Presentation] Comparative genomics of Stx2 prophages in O157:H7 (2012)
  • Author(s): Y. Ogura, T. Mako, T. Ooka, M. Ohnishi, T. Hayashi
  • Organizer: VTEC 2012: 8^th International Symposium on Shiga Toxin (Verocytotoxin) producing Escherichia coli infections
  • Place of Presentation: Amsterdam, The Netherlands.
• [Presentation] 既存のソフトウエアを駆使した実験研究者による微生物ゲノム情報解析 (2012)
  • Author(s): 小椋義俊
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] 腸管侵入性大腸菌のゲノム解析 (2012)
  • Author(s): 小椋義俊,大岡唯祐,磯部順子,河野喜美子, 松本昌門, 勢戸和子, 岩出義人,緒方喜久代, 林哲也
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] Comparative genomics of Stx2 prophages in O157:H7 (2012)
  • Author(s): Y. Ogura, T. Mako, T. Ooka, M. Ohnishi, T. Hayashi
  • Organizer: 8th International Symposium on Shiga Toxin (Verocytotoxin) producing Escherichia coli infections
  • Place of Presentation: Amsterdam
• [Presentation] ゲノム解析から見えてきた腸管出血性大腸菌の病原性進化メカニズム (2011)
  • Author(s): 小椋義俊
  • Organizer: 日本細菌学会関東支部・ICD制度協議会共催「緊急セミナー:腸管出血性大腸菌の今」
  • Place of Presentation: 東京
  • Year and Date: 2011-08-02
• [Presentation] Comparative analysis of STX2 prophages in enterohemorrhagic Escherichia coli O-157 (2011)
  • Author(s): Y. O g u r a, M. Ohnishi, T. Mako, K. Kawano, T. Ooka, T. Hayashi
  • Organizer: UMS 2011(第84回日本細菌学会総会)
  • Place of Presentation: Sapporo
• [Presentation] 腸管出血性大腸菌O157の志賀毒素ファージの比較解析 (2011)
  • Author(s): 小椋義俊
  • Organizer: 第5回細菌学若手コロッセウム
  • Place of Presentation: 高知
• [Book] 第4章 新規ゲノム配列決定編 (2012)
  • Author(s): 小椋義俊, 林哲也
  • Total Pages: 252
  • Publisher: 細菌ゲノム. 細胞工学別冊 次世代シークエンサー 目的別アドバンストメソッド, 監修 菅野純夫/鈴木穣
• [Book] 細胞工学別冊 次世代シークエンサー 目的別アドバンスドメソッド (2012)
  • Author(s): 小椋義俊, 林哲也
  • Total Pages: 252
  • Publisher: 秀潤社
• [Book] 病原菌と常在菌の境界(第6 章腸内共生系の破綻と疾病) (2011)
  • Author(s): 小椋義俊, 林哲也
  • Total Pages: 292
  • Publisher: 丸善出版