| Project/Area Number |
23790501
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | RNA 核外輸送 / HIV-1 / Revタンパク質 / RNA核外輸送 / RNP / スプライシング |
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| Research Abstract |
Human immunodeficiency virus type 1 (HIV-1) Rev protein induces export of the singly spliced and the unspliced HIV-1 transcripts. However, how these RNAs are exported is not well understood, since such transcripts should have both the transcription/export (TREX)complex and Rev on the same RNA, and therefore should have possibility of utilizing the TAP-dependent or CRM1-dependent export, or both. When HIV-1 transcripts were expressed in HEK293T cells, it was suggested that Rev suppresses the TAP recruitment to those transcripts. We also found that Rev interacts with the cap-binding complex (CBC) and inhibits the recruitment of Aly/REF, an adaptor for the TAP-dependent export.
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