Elucidating the physiological meaning of a novel TLR regulatory molecule

• Project/Area Number: 23790526
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: The University of Tokyo
• Principal Investigator: SHIBATA Takuma 東京大学, 医科学研究所, 特任助教 (30554505)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 自然免疫 / TLR

Research Abstract

We identified a novel TLR associating molecule, Epithelial membrane protein (Emp3), and found that knock-down of Emp3 in RAW macrophage cell line resulted in upregulation of multiple TLR response. However, bone marrow derived macrophages from Emp3 KO and Emp3 Tg mice did not show clear differences in various types of TLR response. On the other hand, Emp3 Tg mice died within 6 months and most of them showed Dilated Cardiomyopathy (DCM) like heart symptoms. In addition, some Emp3 Tg mice showed enteritis. It still remained to be determined whether thesephenotypes are associated with unknown TLR function regulated by Emp3 or not.

Research Products

• [Journal Article] PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells (2012)
  • Author(s): Shibata T, Takemura N, Motoi Y, Goto Y, Karuppuchamy T, Izawa K, Li X, Akashi-Takamura S, Tanimura N, Kunisawa J, Kiyono H, Akira S, Kitamura T, Kitaura J, Uematsu S, Miyake K
  • Journal Title: Int Immunol. Volume: 24 Issue: 10 Pages: 613-623
  • DOI: 10.1093/intimm/dxs068
  • Peer Reviewed
• [Presentation] PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells (2012)
  • Author(s): Shibata Takuma, Takemura Naoki, Motoi Yuji, Goto Yoshiyuki, Uematsu Satoshi, Miyake Kensuke.
  • Organizer: 第41回日本免疫学会総会・学術集会
  • Place of Presentation: 神戸ポー トピアホテル、兵庫県
  • Year and Date: 2012-12-06
• [Presentation] PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells (2012)
  • Author(s): Takuma Shibata, Naoki Takemura, Yuji Motoi, Satoshi Uematsu, Kensuke Miyake.
  • Organizer: The 12th Biennial International Endotoxin & Innate Immunity Society(IEIIS) meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2012-10-23
• [Presentation] PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells. (2012)
  • Author(s): Takuma Shibata
  • Organizer: The 12th Biennial International Endotoxin & Innate Immunity Society(IEIIS) meeting
  • Place of Presentation: National Center of Sciences Building, Tokyo
• [Presentation] PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells. (2012)
  • Author(s): Takuma Shibata
  • Organizer: 第41回日本免疫学会総会・学術集会
  • Place of Presentation: 神戸ポートピアホテル
• [Presentation] Intracellular TLR4/MD-2 in macrophagessenses LPS and induces a unique set of TLR4 responses (2011)
  • Author(s): Shibata Takuma, Motoi Yuji, Akashi-Takamura Sachiko and Miyake Kensuke
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-11-28
• [Presentation] Intracellular TLR4/MD-2 in macrophages senses LPS and induces a unique set of TLR4 responses (2011)
  • Author(s): Takuma Shibata
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 幕張メッセ
• [Book] medicina (2013)
  • Author(s): 柴田 琢磨
  • Publisher: 医学書院
• [Book] モデル動物利用マニュアル＜疾患モデルの作製と利用 免疫疾患＞ (2011)
  • Author(s): 柴田 琢磨 他
  • Publisher: Life Science Information Center.
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/ichinohe-lab/index.html