Molecular basis of hematopoietic stem and progenitor niche formation

• Project/Area Number: 23790533
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Kyoto University
• Principal Investigator: OMATSU Yoshiki 京都大学, 再生医科学研究所, 助教 (80437277)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: サイトカイン / 造血幹細胞ニッチ / ニッチ / 造血幹細胞 / ケモカイン / 骨髄

Research Abstract

We have shown that CXC chemokine ligand (CXCL)12-abundant reticular (CAR) cells have potential to differentiate into adipocytes and osteoblasts,and play an essential role in maintenance of hematopoietic stem and progenitor cells (HSPCs) in vivo, meeting the criteria for “niche”. However, themolecular basis of the formation of cellular niches remain unclear. In this study, we found several candidate genes that play essential roles for developmentand maintenance of CAR cells, using microarrayandqRT-PCR analysis, and we generated conditional knock-out mice of these genes.

Research Products

• [Journal Article] Extracellul- ar matrix protein Tenascin-C is req- uired in the bone marrow microenvio- nment primed for hematopoietic rege- neration (2012)
  • Author(s): Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasa- wa T, Suda T, Kubota Y
  • Journal Title: Blood Volume: 119 Issue: 23 Pages: 5429-5437
  • DOI: 10.1182/blood-2011-11-393645
  • Peer Reviewed
• [Journal Article] Control of hematopoietic stem cells by the bone marrow stromal niche : the role of reticular cells (2011)
  • Author(s): Nagasawa, T., Omatsu, Y., Sugiyama, T.
  • Journal Title: Trends Immunol. Volume: 32 Issue: 7 Pages: 315-320
  • DOI: 10.1016/j.it.2011.03.009
  • Peer Reviewed
• [Journal Article] Isolation and function of mouse tissue resident vascular precursors marked by myelin protein zero (2011)
  • Author(s): Kubota Y, Takubo K, Hirashima M, Nagoshi N, Kishi K, Sano K, Murakami M, Ema M, Omatsu Y, Takahashi S, Nagasawa T, Shibuya M, Okano H, Suda T
  • Journal Title: J Exp Med Volume: 208 Issue: 5 Pages: 949-960
  • DOI: 10.1084/jem.20102187
  • Peer Reviewed
• [Journal Article] Isolation and function of mouse tissue resident vascular precursors marked by myelin protein zero. (2011)
  • Author(s): Kubota Y, Takubo K, Hirashima M, Nagoshi N, Kishi K, Okuno Y, Nakamura-Ishizu A, Sano K, Murakami M, Ema M, Omatsu Y, Takahashi S, Nagasawa T, Shibuya M, Okano H, Suda T.
  • Journal Title: J. Exp. Med. Volume: 208(5) Pages: 949-960
  • Peer Reviewed