Roles of Notch signal regulating microRNA in T cell differentiation

• Project/Area Number: 23790538
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: The University of Tokushima
• Principal Investigator: NISHIDA Jun 徳島大学, ヘルスバイオサイエンス研究部, 助教 (00361981)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: miR449a / T cell / Notch / miRNA / Tリンパ球

Research Abstract

We analyzed expression of micro RNA in Notch1 intercellular domain over expressed DO11.10 cells. We identified one of micro RNA, miR449a, which expression was controlled under the Notch signaling. To clarified role of miR449a, we established miR449a knock out mouse. We compared development and maturation of immune competent cell between wild type and miR449a KO mouse. However, we could not find any difference on differentiation of immune competent cell at thymus, spleen and other secondary lymphoid tissue. We also analyzed immune competent cell in small intestinal lamina propria and intraepithelial lymphocytes, we could not find any difference between wild and miR449a KO mouse.