Research of related genes with carcinogenesis of pancreatic cancer using chromosomal analysis

• Project/Area Number: 23790805
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: YOSHIDA Naohisa 京都府立医科大学, 医学研究科, 講師 (50340089)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 胆道癌 / 膵臓癌 / 膵癌 / 染色体転座 / NRG1 / 染色体異常 / 融合遺伝子 / FISH / SKY

Research Abstract

The analyses of spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH) were performed to human pancreatic cell lines. The frequent chromosomal disorder was detected in 8p12. NRG1 in 8p12 was considered as the possibility of fusion gene. The breakpoints of NRG1 was t(8;17)(p11.2;q23), t(8;18)(p23;q12) and t(7;8)(q11.2;p11.2) i(8q). We performed genomic array and bubble PCR to candidate cell lines. The detail breakpoints of NRG1 were exon 1, and exon9-17. We have performed further analyses for these breakpoints to find the fusion gene.

Research Products

• [Journal Article] Multicenter study of endoscopic mucosal resection using 0.13% hyaluronic acid solution of colorectal polyps less than 20 mm in size (2013)
  • Author(s): Yoshida N, Naito Y, Inada Y, Kugai M, Yagi N, Inoue K, Okuda T, Hasegawa D, Kanemasa K, Kyoichi K, Matsuyama K, Ando T, Takemura T, Shimizu S, Wakabayashi N, Yanagisawa A, Yoshikawa T
  • Journal Title: Int J Colorectal Dis Volume: 28 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Possibility of Ex vivo Animal Training Model for Colorectal Endoscopic Submucosal Dissection. (2013)
  • Author(s): Yoshida N, Yagi N, Inada Y, Kugai M, Kamada K, Katada K, Uchiyama K, Ishikawa T, Takagi T, Handa O, Konishi H, Kokura S, Inoue K, Wakabayashi N, Abe Y, Yanagisawa A, Naito Y
  • Journal Title: Int J Colorectal Dis Volume: 28 Pages: 49-56
  • Peer Reviewed
• [Journal Article] 種々の癌腫の抗がん剤治療に伴う末梢神経障害に対する牛車腎気丸の有効性 (2012)
  • Author(s): 吉田直久、細川豊史、石川剛、八木信明、古倉聡、内藤裕二、中西正芳、國場幸均、大辻英吾、黒星晴夫、谷脇雅史、田口哲也、細井創、中村晃和、三木恒治
  • Journal Title: Progress in Medicine Volume: 32 Pages: 2509-2515
  • Peer Reviewed
• [Journal Article] XELOX療法における治療初期の副作用の解析とその対策について (2012)
  • Author(s): 吉田直久、國場幸均、稲田裕、八木信明、古倉聡、内藤裕二、中西正芳、中村晃和、細井創、長谷川大祐、元好貴之、内山清、清水義博、金政和之、谷脇雅史、三木恒治
  • Journal Title: Progress in Medicine Volume: 32 Pages: 721-726
  • Peer Reviewed
• [Journal Article] The Detection of Surface Patterns by Flexible Spectral Imaging Color Enhancement without Magnification for Diagnosis of Colorectal Polyps (2012)
  • Author(s): Yoshida N, Naito Y, Inada Y, Kugai M, Inoue K, Uchiyama K, Handa O, Takagi T, Konishi H, Wakabayashi N, Yagi N, Morimoto Y, Wakabayashi N, Yanagisawa A, Yoshikawa T
  • Journal Title: Int J Colorectal Dis Volume: 27 Pages: 605-611
  • Peer Reviewed
• [Journal Article] The Detection of Surface Patterns by Flexible Spectral Imaging Color Enhancement without Magnification for Diagnosis of Colorectal Polyps (2011)
  • Author(s): Naohisa Yoshida, Yuji Nito, Yutaka Inada, et al.
  • Journal Title: Int J Colorectal Dis. Volume: 27 Pages: 605-611
  • Peer Reviewed