| Project/Area Number |
23790823
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 肝臓病学 / 国際情報交換 / 国際情報交流 |
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| Research Abstract |
Intra-hepatic cholangiocellular carcinoma (IHCCC) is adenocarcinoma,caracterized by the enrichment of fibrous stroma. Fibroblasts, recognized as cancer-associated fibroblasts (CAF), are surrounding tha solid tumor and promote invation and metastasis of the cancer cells. The putative origins of CAF are (1) fibroblast in the organ, (2) bone marrow, (3) epithelial-mesenchymal transition of cancer cells. No investigation for the origin of CAF in IHCCC is reported to date. By the transfection method with the retroviral vector pRetroQ-AcGFPN (Clontech), the human IHCCC cell line (GFP/KMC-1) which expresses GFP protein constantly was established. The clone was characterized by immunohistochemistry and western blotting of epithelial and mesenchymal markers and selected for xenografts in BALB c/nude mice. The optimum injection sites for the tumor formation were subcutaneous tissue and abdominal cavity. Tumor tissue were taken approximately day 20 following xenograft of GFP/KMC -1. The analyssof immunohistochemistry and FISH are underway to investigate the origin of CAF in IHCCC. In the investigation for the expression of EMT genes in the human resected tumors, we found that Snail and Twist genes are expressed at the invasive front of tumors surroundingby CAF.
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