Analysis of differentiation process from human ES/iPS cells into vascular cell components and researches using disease-specific iPS cells with vascular disorders

• Project/Area Number: 23790847
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto University
• Principal Investigator: SONE Masakatsu 京都大学, 医学研究科, 講師 (40437207)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 幹細胞 / iPS細胞 / ES細胞 / 血管 / 再生医療 / 血管内皮細胞 / 血管平滑筋細胞 / 血管生物学

Research Abstract

We clarified the transition of the expression of humoral factors and their receptors in the differentiation process from human ES/iPS cells to vascular cell components.We induced vascular endothelial cells and mural cells from patient-specific iPS cells of inherited vascular disorders, and compared these cells with those of healthy volunteers.In some diseases, we succeeded in detecting a diagnostic marker and showing the significance of a susceptibility gene mutation in vascular cell dysfunction.

Research Products

• [Journal Article] Vascular research using human pluripotent stem cells and humoral factors (2013)
  • Author(s): Sone M, Nakao K
  • Journal Title: Endocr J. Volume: 60(4) Pages: 397-402
  • NAID: 10031170627
• [Journal Article] Human induced pluripotent stem cells differentiated into chondrogenic lineage via generation of mesenchymal progenitor cells (2013)
  • Author(s): Koyama, N., Miura, M., Nakao, K., Kondo, E., Fujii, T., Taura, D., Kanamoto, N., Sone, M., Yasoda, A., Arai, H., Bessho, K., Nakao, K.
  • Journal Title: Stem Cells and Development Volume: 22(1) Issue: 1 Pages: 102-113
  • DOI: 10.1089/scd.2012.0127
  • Peer Reviewed
• [Journal Article] Vascular research using human pluripotent stem cells and humoral factors. (2013)
  • Author(s): Sone M, Nakao K.
  • Journal Title: Endocr J. Volume: 60(4) Pages: 397-402
  • NAID: 10031170627
• [Journal Article] Differentiation of Human Embryonic Stem Cells and Human Induced Pluripotent Stem Cells into Steroid-Producing Cells (2012)
  • Author(s): Sonoyama T, Nakao K, et al
  • Journal Title: Endocrinology Volume: 153 Issue: 9 Pages: 4336-4345
  • DOI: 10.1210/en.2012-1060
  • NAID: 120005385883
  • Peer Reviewed
• [Journal Article] Induced pluripotent stem cells generated from diabetic patients with mitochondrial DNA A3243G mutation (2012)
  • Author(s): J.Fujikura, M.Sone, K.Hosoda, K.Nakao, et al
  • Journal Title: Diabetologia Volume: (in press) Issue: 6 Pages: 1689-1698
  • DOI: 10.1007/s00125-012-2508-2
  • Peer Reviewed
• [Journal Article] A case of myelolipoma with bilateral adrenal hyperaldosteronism cured after unilateral adrenalectomy (2012)
  • Author(s): Inuzuka M, Tamura N, Sone M, Taura D, Sonoyama T, Honda K, Kojima K, Fukuda Y, Ueda Y, Yamashita Y, Kondo E, Yamada G, Fujii T, Miura M, Kanamoto N, Yasoda A, Arai H, Mikami Y, Sasano H, Nakao K.
  • Journal Title: Intern Med Volume: 51(5) Pages: 479-85
  • NAID: 130002061896
  • Peer Reviewed
• [Journal Article] Forkhead box A1 (FOXA1) and A2 (FOXA2) oppositely regulate human type 1 iodothyronine deiodinase gene in liver. (2012)
  • Author(s): Kanamoto N, Tagami T
  • Journal Title: Endocrinology Volume: 153 Issue: 1 Pages: 492-500
  • DOI: 10.1210/en.2011-1310
  • NAID: 120005241346
  • Peer Reviewed
• [Journal Article] A case of myelolipoma with bilateral adrenal hyperaldosteronism cured after unilateral adrenalectomy. (2012)
  • Author(s): Inuzuka M, Tamura N, Sone M, et al.
  • Journal Title: Intern Med. Volume: 51(5):479-85. Pages: 479-85
  • NAID: 130002061896
  • Peer Reviewed
• [Journal Article] Induced pluripotent stem cells generated from diabetic patients with mitochondrial DNA A3243G mutation. (2012)
  • Author(s): Fujikura J, Nakao K, Sone M, Noguchi M, Mori E, Naito M, Taura D, Harada-Shiba M, Kishimoto I, Watanabe A, Asaka I, Hosoda K, Nakao K.
  • Journal Title: Diabetologia Volume: in press
  • Peer Reviewed
• [Journal Article] Forkhead box A1 (FOXA1) and A2 (FOXA2) oppositely regulate human type 1 iodothyronine deiodinase gene in liver. (2012)
  • Author(s): Kanamoto N, Tagami T, Ueda-Sakane Y, Sone M, Miura M, Yasoda A, Tamura N, Arai H, Nakao K.
  • Journal Title: Endocrinology Volume: 153(1) Pages: 492-500
  • NAID: 120005241346
  • Peer Reviewed
• [Journal Article] Significance of adrenocorticotropin stimulation test in the diagnosis of an aldosterone-producing adenoma (2011)
  • Author(s): Sonoyama T, Sone M, Miyashita K, Tamura N, Yamahara K, Park K, Oyamada N, Taura D, Inuzuka M, Kojima K, Honda K, Fukunaga Y, Kanamoto N, Miura M, Yasoda A, Arai H, Itoh H, Nakao K
  • Journal Title: J Clin Endocrinol Metab Volume: 96(9) Issue: 9 Pages: 2771-8
  • DOI: 10.1210/jc.2011-0573
  • Peer Reviewed
• [Journal Article] Simple and highly efficient method for production of endothelial cells from human embryonic stem cells (2011)
  • Author(s): Tatsumi R, Suzuki Y, Sumi T, Sone M, Suemori H, Nakatsuji N
  • Journal Title: Cell Transplant Volume: 20 Issue: 9 Pages: 1423-1430
  • DOI: 10.3727/096368910x547444
  • Peer Reviewed
• [Journal Article] Significance of adrenocorticotropin stimulation test in the diagnosis of an aldosterone-producing adenoma. (2011)
  • Author(s): Sonoyama T, Sone M, Miyashita K, Tamura N, Yamahara K, Park K, Oyamada N, Taura D, Inuzuka M, Kojima K, Honda K, Fukunaga Y, Kanamoto N, Miura M, Yasoda A, Arai H, Itoh H, Nakao K.
  • Journal Title: J Clin Endocrinol Metab. Volume: 96(9) Pages: 2771-8
  • Peer Reviewed
• [Journal Article] Simple and highly efficient method for production of endothelial cells from human embryonic stem cells. (2011)
  • Author(s): Tatsumi R, Suzuki Y, Sumi T, Sone M, Suemori H, Nakatsuji N.
  • Journal Title: Cell Transplant. Volume: 20(9) Pages: 1423-30
  • Peer Reviewed
• [Presentation] ヒトiPS細胞を用いた血管細胞分化研究の疾患解析への応用 (2013)
  • Author(s): 田浦大輔、曽根正勝、長船健二、小嶋勝利、錦見俊雄、本田恭子、荒井宏司、中尾一和
  • Organizer: 第86回 日本内分泌学会学術総会
  • Place of Presentation: 仙台市
  • Year and Date: 2013-04-26
• [Presentation] ヒトES細胞・iPS細胞からのステロイド産生細胞の分化誘導法の開発 (2013)
  • Author(s): 園山拓洋、曽根正勝、本田恭子、田浦大輔、小嶋勝利、犬塚 恵、金本巨哲田村尚久、中尾一和
  • Organizer: 第86回 日本内分泌学会学術総会 若手研究奨励賞
  • Place of Presentation: 仙台市
  • Year and Date: 2013-04-25
• [Presentation] ヒトES/iPS細胞を用いた血管障害の病態解明と創薬の可能性 (2012)
  • Author(s): 曽根正勝、田浦大輔、中尾一和
  • Organizer: 第35回 日本高血圧学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-09-21
• [Presentation] ヒトES細胞/iPS細胞のステロイド産生細胞への分化誘導法の開発 (2012)
  • Author(s): 園山拓洋,曽根正勝,本田恭子,田浦大輔,小嶋勝利,犬塚 恵,金本巨哲,田村尚久,中尾一和
  • Organizer: 第11回 日本再生医療学会学術総会 若手研究奨励賞
  • Place of Presentation: 横浜市
  • Year and Date: 2012-06-12
• [Presentation] ヒトES細胞からの血管細胞分化過程における各種血管ホルモンの発現、作用機序の検討 (2012)
  • Author(s): 田浦大輔、曽根正勝、小嶋勝利、長船健二、園山拓洋、本田恭子、福田賢英、錦見俊雄、中尾一和
  • Organizer: 第85回 日本内分泌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-04-21
• [Presentation] 血管の保護・再生への幹細胞と心血管ホルモンを用いたアプローチ (2012)
  • Author(s): 曽根正勝、中尾一和
  • Organizer: 第85回 日本内分泌学会学術総会 研究奨励賞受賞講演
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-04-20
• [Presentation] The Applicaiton of Human ES/iPS Cell Differentiation System into Vascular Cells for Vascular Pathology (2012)
  • Author(s): 田浦大輔、曽根正勝、錦見俊雄、長船健二、小嶋勝利、園山拓洋、本田恭子、中尾一和
  • Organizer: 第76回 日本循環器学会学術総会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-03-16
• [Presentation] The Applicaiton of Human ES/iPS Cell Differentiation System into Vascular Cells for Vascular Pathology (2012)
  • Author(s): 田浦大輔、曽根正勝、他
  • Organizer: 第76回 日本循環器学会学術総会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] ヒトESおよびiPS細胞を用いた血管細胞分化および血管生理機構 (2011)
  • Author(s): 田浦大輔、曽根正勝、他
  • Organizer: 第15回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府）
• [Presentation] 血管の保護・再生への幹細胞と心血管ホルモンを用いたアプローチ
  • Author(s): 曽根正勝、中尾一和
  • Organizer: 第85回 日本内分泌学会学術総会 研究奨励賞受賞講演
  • Place of Presentation: 名古屋市
• [Presentation] ヒト胚性幹細胞、ヒト誘導多能性幹細胞からのステロイド産生細胞の分化誘導
  • Author(s): 園山 拓洋，曽根 正勝，本田 恭子，田浦 大輔，小嶋 勝利，犬塚 恵，金本 巨哲，田村 尚久，中尾 一和
  • Organizer: 第49回 日本臨床分子医学会学術総会
  • Place of Presentation: 京都市
• [Presentation] ヒトES細胞、iPS細胞を用いた副腎皮質の再生の試み
  • Author(s): 園山拓洋、曽根正勝、本田恭子、田浦大輔、小嶋勝利、犬塚 恵、金本巨哲、田村尚久、中尾一和
  • Organizer: 第109回 日本内科学会総会
  • Place of Presentation: 京都市
• [Presentation] ヒトES細胞/iPS細胞のステロイド産生細胞への分化誘導法の開発
  • Author(s): 園山 拓洋，曽根 正勝，本田 恭子，田浦 大輔，小嶋 勝利，犬塚 恵，金本 巨哲，田村 尚久，中尾 一和
  • Organizer: 第11回 日本再生医療学会学術総会
  • Place of Presentation: 横浜市
• [Book] Gene therapy and regenerative medicine for metabolic syndrome (2011)
  • Author(s): Sonoyama T, Sone M, Nakao K
  • Publisher: Nihon Rinsho
• [Patent(Industrial Property Rights)] METHOD OF EXAMINING POLYCYSTIC KIDNEY DISEASE AND METHOD OF SCREENING FOR THERAPEUTIC AGENT OF THE DISEASE (2011)
  • Inventor(s): 長船健二、塩田文彦、豊田太郎、中尾一和、曽根正勝、田浦大輔
  • Industrial Property Rights Holder: 京都大学、長船健二、塩田文彦、豊田太郎、中尾一和、曽根正勝、田浦大輔
  • Filing Date: 2011-11-07
  • Overseas
• [Patent(Industrial Property Rights)] MITOCHONDRIAL DISEASE-SPECIFIC INDUCED PLURIPOTENT STEM CELLS, METHOD OF PRODUCING SAME AND USE THEREOF (2011)
  • Inventor(s): 中尾一和、藤倉純二、細田公則、曽根正勝、中尾一泰
  • Industrial Property Rights Holder: 京都大学、中尾一和、藤倉純二、細田公則、曽根正勝、中尾一泰
  • Filing Date: 2011-06-20
  • Overseas