Platelet-derived microparticles augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients

• Project/Area Number: 23790886
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Circulatory organs internal medicine
• Research Institution: Kurume University
• Principal Investigator: OHTSUKA MASANORI 久留米大学, 医学部, 助教 (30461429)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 血管新生細胞 / 血小板由来膜小胞体 / RANTES / 血管新生 / 循環器内科学 / 分子血管病態学 / 血管内皮前駆細胞

Research Abstract

We investigated whether PMPs could augment the neovascularization. We isolated mononuclear cells and PMPs from atherosclerotic patient-derived peripheral blood and generated PMP-pretreated CACs(PMP-CACs) by co-culture of the mononuclear cells and PMPs. The adhesion capacity of PMP-CACs was greater to that of CACs. PMPs released RANTES into the culture medium. Intravenous injection of PMP-CACs to rats with hindlimb ischemia augmented neovascularization of the ischemic limbs more than the injection of CACs.The number of PMP-CACs incorporated into the capillaries of the ischemic limbs was greater than that of incorporated CACs.The augmented adhesion and neovascularization capacities by PMP-CACs were canceled out by a RANTES neutralizing antibody.
PMP-secreted RANTES may play role in the augmenting adhesion and neovascularization capacities of CACs. Injection of PMP-CACs may be a new strategy to augment the effects of therapeutic angiogenesis for limb ischemia in atherosclerotic patients.

Research Products

• [Journal Article] Platelet-derived microparticles augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients (2013)
  • Author(s): 大塚昌紀、佐々木健一郎、今泉勉
  • Journal Title: Atherosclerosis Volume: 227巻 Issue: 2 Pages: 275-282
  • DOI: 10.1016/j.atherosclerosis.2013.01.040
  • Peer Reviewed
• [Journal Article] Platelet-derived microparticles augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients (2013)
  • Author(s): 大塚 昌紀
  • Journal Title: Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ Volume: 227 Pages: 275-282
  • Peer Reviewed
• [Presentation] Platelet-derived microparticles augment the adhesion and neovascularization capacities of circulating angiogenic cells obtained from atherosclerotic patients (2013)
  • Author(s): 大塚昌紀
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2013-03-18
• [Presentation] Platelet-derived microparticles augmented neovascularization capacities of endothelial progenitor cells
  • Author(s): 大塚 昌紀
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 血小板由来膜小胞体は血管内皮前駆細胞の血管新生能力を増強させた
  • Author(s): 大塚 昌紀
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡国際センター