| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
GABARAP (gamma-aminobutyric acid A receptor-associated protein) has recently been characterized as an autophagy related protein, though its precise role in autophagy remains unclear. We recently generated green fluorescent protein (GFP)-GABARAP transgenic (Tg) mice, in which GFP-GABARAP is abundantly expressed in glomerular podocytes. Adriamycin (ADR) treatment which causes glomerulosclerosis resulted in a significant increase in proteinuria and sclerotic glomeruli in Tg mice when compared to wild type (WT) mice. Our results indicate that GABARAP is responsible for the impairment of glomerular function, and that it retards recovery from the effects of ADR.
Next, we produced podocyte-specific Atg7 knockout (KO) mice in which autophagy is suppressed. ADR treatment produced significantly worse podocyte injury to KO mice than to WT mice.
Presently, we are continuing to elucidate the relationship between podocyte injury and autophagy deficiency in the podocyte.
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