Elucidation of the relationship between autophagy and glomerulosclerosis caused by podocyte injury
Project/Area Number |
23790955
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Kidney internal medicine
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Research Institution | Juntendo University |
Principal Investigator |
ASANUMA Etsuko 順天堂大学, 医学部, 非常勤助教 (30508172)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | ポドサイト / オートファジー / アポトーシス / アドリアマイシン腎症 / Atg7 / GABARAP / デンドリン |
Research Abstract |
GABARAP (gamma-aminobutyric acid A receptor-associated protein) has recently been characterized as an autophagy related protein, though its precise role in autophagy remains unclear. We recently generated green fluorescent protein (GFP)-GABARAP transgenic (Tg) mice, in which GFP-GABARAP is abundantly expressed in glomerular podocytes. Adriamycin (ADR) treatment which causes glomerulosclerosis resulted in a significant increase in proteinuria and sclerotic glomeruli in Tg mice when compared to wild type (WT) mice. Our results indicate that GABARAP is responsible for the impairment of glomerular function, and that it retards recovery from the effects of ADR. Next, we produced podocyte-specific Atg7 knockout (KO) mice in which autophagy is suppressed. ADR treatment produced significantly worse podocyte injury to KO mice than to WT mice. Presently, we are continuing to elucidate the relationship between podocyte injury and autophagy deficiency in the podocyte.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Translocation of dendrin to the podocyte nucleus in acute glomerular injury in patoents with IgA nephropathy2013
Author(s)
Kodama F, Asanuma K, Takagi M, Hidaka T, Asanuma E, Fukuda H, Seki T, Takeda Y, Hosoe-Nagai Y, Asao R, Horikoshi S, Tomino Y
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Journal Title
Nephrol Dial Transplant
Volume: 28
Pages: 1762-72
Related Report
Peer Reviewed
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[Journal Article] Translocation of dendrin to the podocyte nucleus in acute glomerular injury in patoents with IgA nephropathy.2013
Author(s)
Kodama F, Asanuma K, Takagi M, Hidaka T, Asanuma E, Fukuda H, Seki T, Takeda Y, Hosoe-Nagai Y, Asao R, Horikoshi S, Tomino Y
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Journal Title
Nephrol Dial Transplant
Volume: 28
Pages: 1762-72
Related Report
Peer Reviewed
-
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[Journal Article] ranslocation of dendrin to the podocyte nucleus in acute glomerular injury in patoents with IgA nephropathy.2012
Author(s)
Kodama F, Asanuma K, Takagi M, Hidaka T, Asanuma E, Fukuda H, Seki T, Takeda Y, Hosoe-Nagai Y, Asao R, Horikoshi S, Tomino Y
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Journal Title
Nephrol Dial Transplant.
Volume: 28
Pages: 1762-72
Related Report
Peer Reviewed
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[Journal Article] Dendrin location in podocytes is associated with the disease progression in animal and human glomerulopathy2011
Author(s)
Asanuma K, Akiba-Takagi M, Kodama F, Asao R, Nagai Y, Lydia A, Fukuda H, Tanaka E, Shibata T, Takahara H, Hidaka T, Asanuma E, Kominami E, Ueno T, Tomino Y
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Journal Title
Am J Nephrol
Volume: 23
Pages: 537-549
Related Report
Peer Reviewed
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