| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Intracellular trafficking is a fundamental cellular process that is required for communication between organelles. Recruitment of specific molecules to specific membrane sites is essential for this trafficking. Rab27a, a member of the Rab GTPase family, has been considered to act as a molecular switch for exocytosis in insulin secretion, cycling between an active GTP-bound and an inactive GDP-bound state. I previously showed that GDP-bound Rab27a is not an inactive form but is in fact an active form that regulates endocytosis through the GDP-dependent effector coronin3. In the present study, I identified IQGAP1, an effector of GTP-bound Cdc42, as another GDP-dependent effector of Rab27a. My results indicate that activation of Cdc42 in response to the insulin secretagogue glucose recruits endocytic machinery, including GDP-bound Rab27a and coronin3, to IQGAP1 at the cell periphery and regulates endocytosis at this membrane site.
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