A novel GDP-bound-Rab27a-interacting protein which regulates endocytosis of insulin secretory membranes
| Project/Area Number |
23791039
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | IQGAP1 / Rab27a / エンドサイトーシス / 膵B細胞 / インスリン / 糖尿病 / Gタンパク質 / メンブレントラフィック / 低分子量Gタンパク質 / coronin |
|---|
| Research Abstract |
Intracellular trafficking is a fundamental cellular process that is required for communication between organelles. Recruitment of specific molecules to specific membrane sites is essential for this trafficking. Rab27a, a member of the Rab GTPase family, has been considered to act as a molecular switch for exocytosis in insulin secretion, cycling between an active GTP-bound and an inactive GDP-bound state. I previously showed that GDP-bound Rab27a is not an inactive form but is in fact an active form that regulates endocytosis through the GDP-dependent effector coronin3. In the present study, I identified IQGAP1, an effector of GTP-bound Cdc42, as another GDP-dependent effector of Rab27a. My results indicate that activation of Cdc42 in response to the insulin secretagogue glucose recruits endocytic machinery, including GDP-bound Rab27a and coronin3, to IQGAP1 at the cell periphery and regulates endocytosis at this membrane site.
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Report
(4 results)
Research Products
(51 results)
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[Journal Article] Activated Cdc42-bound-IQGAP1 determines the cellular endocytic site2013
Author(s)
Kimura T, Yamaoka M, Taniguchi S, Okamoto M, Takei M, Ando T, Iwamatsu A, Watanabe T, Kaibuchi K, Ishizaki T, Niki I
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Journal Title
Mol. Cell. Biol
Volume: 33
Issue: 24
Pages: 4834-43
DOI
Related Report
Peer Reviewed
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[Journal Article] Activated Cdc42-bound-IQGAP1 determines the cellular endocytic site2013
Author(s)
Kimura T, Yamaoka M, Taniguchi T, Okamoto M, Takei M, Ando T, Iwamatsu A, Watanabe T, Kaibuchi K, Ishizaki T, Niki I
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Journal Title
Mol. Cell. Biol.
Volume: 33
Pages: 4834-4843
Related Report
Peer Reviewed
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[Journal Article] Distribution of hydrogen sulfide (H2S)-producing enzymes and the roles of the H)S donor sodium hydrosulfide in diabetic nephropathy.2013
Author(s)
Yamamoto J, Sato W, Kosugi T, Yamamoto T, Kimura T, Taniguchi S, Kojima H, Maruyama S, Imai E, Matsuo S, Yuzawa Y, Niki I
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Journal Title
Clin Exp Nephrol.
Volume: 17
Pages: 32-40
Related Report
Peer Reviewed
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[Book] 生化学2013
Author(s)
木村俊秀、山岡真美
Total Pages
5
Publisher
日本生化学会
Related Report
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[Book] 日本臨床2013
Author(s)
岡本光弘、山岡真美、木村俊秀
Total Pages
6
Publisher
日本臨床社
Related Report
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