Interaction between monocytes and bone marrow microenvironment in pathogenesis of multiple myeloma

• Project/Area Number: 23791084
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Sapporo Medical University
• Principal Investigator: IKEDA HIROSHI 札幌医科大学, 道民医療推進学講座, 特任助教 (60570132)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 多発性骨髄腫 / 骨髄微小環境 / 単球 / 骨髄ストローマ細胞

Research Abstract

In this study, we examined the role of monocyte, a component of bone marrow microenvironment, in the MM progression. We investigated the proliferation of MM cell lines cultured alone or co-cultured with BMSCs and/or monocytes of MM patients. Consistently, we observed increased proliferation of MM cell lines in the presence of either BMSCs or monocytes compared to cell line-only control. Furthermore, the co-culture of BMSCs plus monocytes induced the greatest degree of proliferation of myeloma cells. In addition to increased proliferation, BMSCs and monocytes decreased the rate of apoptosis of myeloma cells. Our results therefore suggest that highlights the role of monocyte as an important component of the bone marrow microenvironment.

Research Products

• [Journal Article] Genomic vulnerability to LINE-1 hypomethylation is a potential determinant of the clinicogenetic features of multiple myeloma (2013)
  • Author(s): Nojima M, Suzuki H, Yasui H, Maruyama R, Yamamoto E, Ashida M, Itagaki M, Asaoku H, Ikeda H, Hayashi T, Imai K, Mori M, Tokino T, Ishida T, Toyota M, Shinomura Y
  • Journal Title: Genome Medicine Volume: 2013年10月号 Pages: 88-88
  • Peer Reviewed
• [Journal Article] Usefulness of serum free light-chain assay for evaluation of therapeutic efficacy in non-secretory multiple myeloma (2013)
  • Author(s): Maruyama Y, Hayashi T, Ikeda H, Yasui H, Ishida T, Shinomura Y
  • Journal Title: 臨床血液査 Volume: 1月号 Pages: 122-122
  • NAID: 10031147829
  • Peer Reviewed
• [Journal Article] Correction: Genomic vulnerability to LINE-1 hypomethylation is a potential determinant of the clinicogenetic features of multiple myeloma. (2013)
  • Author(s): Aoki Y, Itagaki M, Asaoku H, Ikeda H, Hayashi T, Imai K, Mori M, Tokino T, Ishida T, Toyota M, Shinomura Y.
  • Journal Title: Genome Med. Volume: 10 Pages: 88-92
  • Peer Reviewed
• [Journal Article] Model of translational cancer research in multiple myeloma (2012)
  • Author(s): Yasui H, Ishida T, Maruyama R, Nojima M, Ikeda H, Suzuki H, Hayashi T, Shinomura Y, Imai K
  • Journal Title: Cancer Science Volume: 2012年11月号 Pages: 1907-1907
  • Peer Reviewed
• [Presentation] Target drug of symptomatic myeloma in bone marrow microeviroment (2013)
  • Author(s): 池田博
  • Organizer: 日本がん学会
  • Place of Presentation: 横浜
• [Presentation] THE RELATIONSHIP OF MYELOMA CELLS, STROMAL CELLS AND MONOCYTES UNDER BONE MARROW MICROENVIRONMENT (2013)
  • Author(s): Hiroshi Ikeda
  • Organizer: European hematology association
  • Place of Presentation: 都市 ストックホルム スエーデン
• [Presentation] Analysis of interactions myeloma cells, bone marrow stromal cells and monocyte (2012)
  • Author(s): 池田 博
  • Organizer: 日本癌学会
  • Place of Presentation: ロイトン札幌
• [Presentation] CD138-specific monoclonal antibody conjugated with highly cytotoxic maytansinoid derivative is effective to Multiple myeloma cells in Bone marrow microenviroment. (2011)
  • Author(s): 池田 博
  • Organizer: 日本がん学会
  • Place of Presentation: 名古屋
• [Book] 日本臨床血液症候群IIIgM型多発性骨髄腫 (2013)
  • Author(s): 池田博
• [Book] 血液症候群 (2013)
  • Author(s): 池田 博、石田 禎夫
  • Total Pages: 5
  • Publisher: 日本臨床