Anti-G-CSF autoantibodies regulate neutrophil function in patiernt with bacterial pneumonia

Research Project

Project/Area Number	23791119
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	膠原病・アレルギー・感染症内科学
Research Institution	Kyorin University
Principal Investigator	MASATO Watanbe 杏林大学, 医学部, その他 (00458902)
Research Collaborator	UCHIDA Kanji NAKAGAKI Kazuhide NAKATA Koh GOTO Hajime
Project Period (FY)	2011-04-28 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	自己抗体 / G-CSF / 免疫複合体 / 細菌性肺炎 / IVIG / 健常者 / 市中肺炎 / キャリアプロテイン / 抗G-CSF自己抗体 / 肺炎 / 好中球
Outline of Final Research Achievements	We developed a novel method to measure serum levels of anti-G-CSF autoantibody (G-CSF autoantibody) which conventional method failed to quantify. Both healthy individuals and patients with bacterial pneumonia had G-CSF autoantibodies in their sera. Serum G-CSF autoantibody levels positively correlated with white blood cell counts, neutrophil counts, and C-reactive protein levels. G-CSF autoantibodies did not neutralize G-CSF bioactivity (non-neutralizing antibody). G-CSF-autoantibody immune complex were present in sera of pneumonia patients. Thus, G-CSF autoantibodies works as carrier protein for G-CSF.