Seek for the bioactive substance in human milk

• Project/Area Number: 23791164
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: FUJISAWA Yasuko 浜松医科大学, 医学部附属病院, 助教 (40402284)
• Research Collaborator: 岩原 邦宏 浜松医科大学医学部, 検査部 ; 中川 祐一 浜松医科大学医学部, 小児科 ; MATSUMURA Davis Fumio University of California
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 栄養 / 脂肪細胞 / 母乳 / 小児肥満 / DOHad / DOHaD / 脂肪細胞分化 / SCD１ / 小児栄養 / 生理活性脂質 / 脂肪酸 / 脂質生物学

Outline of Final Research Achievements

The direct effect of human milk on differentiation of 3T3-L1 preadipocytes was investigated. The induction of preadipocyte differentiation by human milk lipids was tested in comparison to the cells induced by infant formula lipids. It was clearly shown that lipids in human milk initiate preadipocyte differentiation. The expression of late adipocyte markers in day 7 adipocytes (initiated into differentiation by human milk lipid at day 0) was comparable with those in control cells initiated by standard method. Furthermore, I tried to see the molecular components in lipid droplets in cells induced by human milk lipid by imaging mass spectrometry. The methods for sample preparation has been established, however, to obtain some reliable results, more efforts for condition setting should be necessary.
The results suggest that matured adipocytes loosing the ability of proliferation compared to preadipocytes, induced by human milk lipid during early life pirioid,

Research Products

• [Journal Article] The lipid fraction of human milk initiates adipocyte differentiation in 3T3-L1 cells. (2015)
  • Author(s): Yasuko Fujisawa, Rie Yamaguchi, Eiko Nagata, Eiichiro Satake, Shinichirou Sano, Rie Matsushita, Kazuteru Kitsuta, Shinichi Nakashima, Toshiki Nakanishi, Yuichi Nakagawa, Tsutomu Ogata.
  • Journal Title: Early Human Development Volume: 89 Pages: 713-719
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Impact of a novel homozygous mutation in nicotinamide nucleotide transhydrogenase on mitochondrial DNA integrity in a case of familial glucocorticoid deficiency. (2015)
  • Author(s): Fujisawa Y, Napoli E, Wong S, Song G, Yamaguchi R, Matsui T, Nagasaki K, Ogata T, Giulivi C
  • Journal Title: BBA clinical Volume: 3 Pages: 70-78
  • DOI: 10.1016/j.bbacli.2014.12.003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The lipid fraction of human milk initiates adipocyte differentiation in 3T3-L1 cells. (2013)
  • Author(s): Fujisawa Y*, Yamaguchi R, Nagata E, Satake E, Sano S, Matsushita R, Kitsuta K, Nakashima S, Nakanishi T, Nakagawa Y, Ogata T
  • Journal Title: Early Hum Dev Volume: Epub ahead of print Issue: 9 Pages: 713-719
  • DOI: 10.1016/j.earlhumdev.2013.05.002
  • Peer Reviewed
• [Journal Article] The lipid fraction of human milk initiates adipocyte differentiation in 3T3-L1 cells (2013)
  • Author(s): Yasuko Fujisawa, Rie Yamaguchi, Eiko Nagata, Eiichiro Satake, Sano Shinichiro, Rie Matsushita, Kazunobu Kitsuta, Shinichi Nakashima, Toshiki Nakanishi, Yuichi Nakagawa, Tsutomu Ogata
  • Journal Title: Early human development Volume: in printing
  • Peer Reviewed
• [Presentation] メタボリックシンドローム発症予防における母乳栄養の有用性に関する基礎的研究" 日本内分泌学会学術集会(招待講演). (平成２４年４月１９日). 名古屋 (2014)
  • Author(s): 藤澤泰子
  • Organizer: 日本内分泌学会学術集会(招待講演)
  • Place of Presentation: 名古屋
  • Year and Date: 2014-04-09
  • Invited
• [Presentation] メタボリックシンドローム発症予防における母乳栄養の有用性に関する基礎的研究
  • Author(s): 藤澤泰子
  • Organizer: 第８４回日本内分泌学会 学術集会
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] メタボリックシンドローム発症予防における母乳栄養の有用性に関する基礎的研究
  • Author(s): 藤澤泰子
  • Organizer: 日本内分泌学会学術集会(招待講演)
  • Place of Presentation: 名古屋