Construction of validation system for regenerative therapy by glycan profiling technology

• Project/Area Number: 23791210
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: INOUE Mayu 独立行政法人国立成育医療研究センター, 細胞医療研究部, 研究員 (40596920)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 再生医療 / 幹細胞 / レクチン / 糖鎖プロファイリング / 間葉系幹細胞 / 多能性幹細胞 / 体性幹細胞 / 品質評価 / 糖鎖 / 細胞プロファイリング / 細胞品質管理 / 間葉系細胞 / 増殖能 / 分化能 / 糖鎖融解

Outline of Final Research Achievements

The cell surface is covered with various glycoproteins, which play a crucial role in biological function. In this study, I aimed to define the status of somatic stem cells and pluripotent stem cells using glycan profiles. The glycan profiles promises to be of great value of stem cell validation for regenerative therapy, complementing more established methods such as gene expression analysis and epigenetic analysis.

Research Products

• [Journal Article] Ataxia telangiectasia derived iPS cells show preserved x-ray sensitivity and decreased chromosomal instability (2014)
  • Author(s): Fukawatase Y, Toyoda M, Okamura K, Nakamura K, Nakabayashi K, Takada S, Yamazaki-Inoue M, Masuda A, Nasu M, Hata K, Hanaoka K, Higuchi A, Takubo K, Umezawa A
  • Journal Title: Sci. Rep. Volume: 4 Issue: 1 Pages: 5421-5421
  • DOI: 10.1038/srep05421
  • Peer Reviewed / Open Access
• [Journal Article] Large-scale cell production of stem cells for clinical application using the automated cell processing machine. (2013)
  • Author(s): Kami D, Watakabe K, Yamazaki-Inoue M, Minami K, Kitani T, Itakura Y, Toyoda M, Sakurai T, Umezawa A, Gojo S.
  • Journal Title: BMC Biotechnol Volume: 13 Issue: 1 Pages: 102-102
  • DOI: 10.1186/1472-6750-13-102
  • Peer Reviewed
• [Journal Article] Glycan profiling of endometrial cancers using lectin microarray. (2012)
  • Author(s): Nishijima Y, Toyoda M, Yamazaki-Inoue M, Sugiyama T, Miyazawa M, Muramatsu T, Nakamura K, Narimatsu H, Umezawa A, and Mikami M.
  • Journal Title: Genes Cells Volume: 17 Issue: 10 Pages: 826-836
  • DOI: 10.1111/gtc.12003
  • Peer Reviewed
• [Presentation] ヒトiPS細胞の異常メチル化領域の解析 (2013)
  • Author(s): 西野光一郎・豊田雅士・山﨑-井上麻由・阿久津英憲・梅澤明弘
  • Organizer: 第7回 日本エピジェネティクス研究会年会
  • Place of Presentation: 奈良
• [Presentation] ヒトiPS細胞の未分化マーカーとなる新規糖鎖の発現解析 (2013)
  • Author(s): 柴田恵理、尾島琢磨、豊田雅士、中島英規、井上麻由、清河信敬、藤本純一郎、佐藤智典、梅澤明弘
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: Yokohama
• [Presentation] LS-MSを用いたヒトiPS細胞に発現する新規糖鎖マーカーの探索 (2012)
  • Author(s): 柴田恵理、尾島琢磨、豊田雅士、中島英規、井上麻由、清河信敬、藤本純一郎、梅澤明弘、佐藤智典
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] Monitoring of the damage to freeze-thawed cells by glycan profiling using a lectin microarray. (2012)
  • Author(s): Kami D, Toyoda M, Yamazaki-Inoue M, Itakura Y, Watakabe K, Sakurai T, Takehara N, Ogata T, Matsubara H, Umezawa A, and Gojo S
  • Organizer: International Society for Stem Cell Research 10th Annual Meeting
  • Place of Presentation: Yokohama
• [Presentation] Construction of large amounts culture system by an automated cell processing machine for the cell transplantation. (2012)
  • Author(s): Kami D, Toyoda M, Yamazaki-Inoue M, Takehara N, Ogata T, Matsubara H, Umezawa A, and Gojo S
  • Organizer: International Society for Stem Cell Research 10th Annual Meeting
  • Place of Presentation: Yokohama
• [Presentation] Investigation of glycosphingolipids expressed in human induced pluripotent stem (iPS) cells. (2012)
  • Author(s): Shibata E, Ojima T, Toyoda M, Nakajima H, Yamazaki-Inoue M, Kiyokawa N, Fujimoto J, Umezawa A, and Sato T.
  • Organizer: International Symposium on Glyco-minded Biology of Diseases as a Basis of Pharmaceutical Sciences,
  • Place of Presentation: Tokyo