Involvement of the type III sodium phosphate cotransporter Pit-1 in phosphate reabsorption

• Project/Area Number: 23791216
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Research Institute, Osaka Medical Center for Maternal and Child Health
• Principal Investigator: YAMAZAKI Miwa 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), その他部局等, 研究員 (50455549)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: Pit-1 / リン / りん / くる病

Outline of Final Research Achievements

Type III sodium phosphate cotransporter, Pit-1, is ubiquitously expressed in mammalian tissues and plays a role as intracellular phosphate transporter, although the function of Pit-1 remains unclear. We have reported that extracellular phosphate and FGF23, a phosphaturic hormone, trigger the signal transduction via Pit-1 and FGF receptor and the signaling shares the same downstream cascade and interacts. In the current study, we have isolated the cells of osteoblasts and osteocytes lineage from Hyp mice, a murine model for X-linked hypophosphatemia, and wild type mice, and obtained the Fgf23 and Pit-1 expression profiles. Increased Pit-1 expression in osteoblasts and osteocytes from adult Hyp mice may be a compensatory mechanism to adapt to the decrease in extracellular Pi levels after birth.

Research Products

• [Journal Article] Interleukin-1-induced acute bone resorption facilitates the secretion of fibroblast growth factor 23 into the circulation. (2014)
  • Author(s): Yamazaki M, Kawai M, Miyagawa K, Ohata Y, Tachikawa K, Kinoshita S, Nishino J, Ozono K, Michigami T.
  • Journal Title: J Bone Miner Metab. Volume: 0 Issue: 3 Pages: 0-0
  • DOI: 10.1007/s00774-014-0598-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dysregulated gene expression in the primary osteoblasts and osteocytes isolated from hypophosphatemic Hyp mice. (2014)
  • Author(s): Miyagawa K, Yamazaki M, Kawai M, Nishino J, Koshimizu T, Ohata Y, Tachikawa K, Mikuni-Takagaki Y, Kogo M, Ozono K, Michigami T
  • Journal Title: PLoS One Volume: 9 Issue: 4 Pages: 0-0
  • DOI: 10.1371/journal.pone.0093840
  • Peer Reviewed / Open Access
• [Journal Article] Elevated fibroblast growth factor 23 exerts its effects on placenta and regulates vitamin D metabolism in pregnancy of hyp mice (2014)
  • Author(s): Okano T(12), 他13人
  • Journal Title: J Bone Miner Res Volume: 印刷中 Issue: 7 Pages: 0-0
  • DOI: 10.1002/jbmr.2186
  • Peer Reviewed / Open Access
• [Journal Article] Sodium-coupled Neutral Amino Acid Transporter 4 Functions as a Regulator of Protein Synthesis during Liver Development. (2013)
  • Author(s): Kondou H, Kawai M, Tachikawa K, Kimoto A, Yamagata M, Koinuma T, Yamazaki M, Nakayama M, Mushiake S, Ozono K, Michigami T
  • Journal Title: Hepatol Res Volume: 43 Issue: 11 Pages: 1211-1223
  • DOI: 10.1111/hepr.12069
  • Peer Reviewed
• [Journal Article] FGF23 suppresses chondrocyte proliferation in the presence of soluble α-Klotho both in vitro and in vivo. (2013)
  • Author(s): Kawai M, Kinoshita S, Kimoto A, Hasegawa Y, Miyagawa K, Yamazaki M, Ohata Y, Ozono K, Michigami T.
  • Journal Title: J Biol Chem Volume: 288 Issue: 4 Pages: 2414-2427
  • DOI: 10.1074/jbc.m112.410043
  • Peer Reviewed
• [Presentation] X連鎖性低リン血症性くる病マウスモデルHypの骨細胞においてはFGF/FGFRシグナルが活性化している (2014)
  • Author(s): 道上敏美，宮川和晃，西野仁，山﨑美和，川井正信，大幡泰久，立川加奈子，高垣裕子，古郷幹彦，大薗惠一
  • Organizer: 第48回日本小児内分泌学会学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Activation of FGF/FGF receptor signaling in the primary osteocytes isolated from hypophosphatemic Hyp mice (2014)
  • Author(s): Miyagawa K, Yamazaki M, Kawai M, Koshimizu T, Nshino J, Ohata Y, Tachikawa K, Mikuni-Takagaki Y, Kogo M, Ozono K, Michigami T
  • Organizer: The 36th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Houston, USA
  • Year and Date: 2014-09-12 – 2014-09-15
• [Presentation] Extracellular inorganic phosphate functions as a potent inducer of the Dmp1 expression (2014)
  • Author(s): Nishino J, Miyagawa K, Yamazaki M, Kawai M, Ohata Y, Tachikawa K, Mikuni-Takagaki Y, Kogo M, Ozono K, Michigami T
  • Organizer: The 36th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Houston, USA
  • Year and Date: 2014-09-12 – 2014-09-15
• [Presentation] Activation of FGF/FGF receptor signaling in Hyp osteocytes (2014)
  • Author(s): Nishino J, Miyagawa K, Yamazaki M, Kawai M, Ohata Y, Tachikawa K, Mikuni-Takagaki Y, Kogo M, Ozono K, Michigami T
  • Organizer: The 24th Annual Meeting of the Australian and New Zealand Bone and Mineral Society
  • Place of Presentation: Queenstown, New Zealand
  • Year and Date: 2014-09-07 – 2014-09-10
• [Presentation] Hypマウス骨細胞におけるFGF/FGF receptorシグナルの活性化 (2014)
  • Author(s): 西野仁，宮川和晃，山﨑美和，川井正信，大幡泰久，立川加奈子，高垣裕子，古郷幹彦，大薗惠一，道上敏美
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] 細胞外無機リン酸はDmp1の発現を誘導し骨細胞分化を促進する (2014)
  • Author(s): 西野仁，宮川和晃，川井正信，山﨑美和，立川加奈子，高垣裕子，古郷幹彦，大薗惠一，道上敏美
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] FGF23の血中分泌過程における破骨細胞性骨吸収の関与の可能性 (2012)
  • Author(s): 山崎美和
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
• [Presentation] Osteoclastic Bone Resorption might be Involved in the Secretion of FGF23 into Circulation (2012)
  • Author(s): 山崎美和
  • Organizer: The 34th annual meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Minneapolis, U.S.A.
• [Remarks] 研究内容、環境影響部門、大阪府立母子保健総合医療センター研究所
  • URL: http://www.mch.pref.osaka.jp/research/bone/content.html
• [Remarks] 業績、環境影響部門、大阪府立母子保健総合医療センター研究所
  • URL: http://www.mch.pref.osaka.jp/research/bone/work.html
• [Remarks] 研究内容、環境影響部門、大阪府立母子保健総合医療センター研究所
  • URL: http://www.mch.pref.osaka.jp/research/bone/content.html
• [Remarks] 業績、環境影響部門、大阪府立母子保健総合医療センター研究所
  • URL: http://www.mch.pref.osaka.jp/research/bone/work.html
• [Remarks] 研究内容、環境影響部門、大阪府立母子保健総合医療センター研究所
  • URL: http://www.mch.pref.osaka.jp/laboratory/environment/content.html
• [Remarks] 業績、環境影響部門、大阪府立母子保健総合医療センター研究所
  • URL: http://www.mch.pref.osaka.jp/laboratory/environment/work.html