Epigenomic analysis and development of therapeutic target for malignant melanoma
| Project/Area Number |
23791283
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
SATO AKIKO 札幌医科大学, 医学部, 助教 (10468093)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 悪性黒色腫 / 癌幹細胞 / エピジェネティック |
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| Research Abstract |
To identify subpopulation having stem cell characteristics in melanoma, we isolated cells by fluorescence-activated sorting and implanted cells to NOD/SCID mouse and performed sphere formation assay. ABCB5 positive subpopulation in AKI melanoma cells and CD271 positive cells in WM266 cells were more tumorigenic than CD271 negative cells or ABCB5 negative cells when grafted to mice. We also analyzed slow cycling, label retaining cell. The rate of sphere formation of label retaining cells was higher than the other cells and label retaining cells showed increased expression of CD271 and ABCB5. These result show these subpopulation in melanoma cells have stem cell characteristics. We think additional analysis of epigenetic change of these cells can be used to detect new therapeutic target for melanoma.
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Report
(4 results)
Research Products
(5 results)
