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Epigenomic analysis and development of therapeutic target for malignant melanoma

Research Project

Project/Area Number 23791283
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionSapporo Medical University

Principal Investigator

SATO AKIKO  札幌医科大学, 医学部, 助教 (10468093)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords悪性黒色腫 / 癌幹細胞 / エピジェネティック
Research Abstract

To identify subpopulation having stem cell characteristics in melanoma, we isolated cells by fluorescence-activated sorting and implanted cells to NOD/SCID mouse and performed sphere formation assay. ABCB5 positive subpopulation in AKI melanoma cells and CD271 positive cells in WM266 cells were more tumorigenic than CD271 negative cells or ABCB5 negative cells when grafted to mice. We also analyzed slow cycling, label retaining cell. The rate of sphere formation of label retaining cells was higher than the other cells and label retaining cells showed increased expression of CD271 and ABCB5. These result show these subpopulation in melanoma cells have stem cell characteristics. We think additional analysis of epigenetic change of these cells can be used to detect new therapeutic target for melanoma.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] Aberrant methylation of microRNA-34b/c is a predictive marker of metachronous gastric cancer risk2013

    • Author(s)
      Suzuki R, Nosho K (他14名), Yamamoto H, Shinomura Y
    • Journal Title

      J Gastroenterol

      Volume: (Epub ahead of print) Issue: 7 Pages: 1135-1144

    • DOI

      10.1007/s00535-013-0861-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular Dissection of Premalignant Colorectal Lesions Reveals Early Onset of the CpG Island Methylator Phenotype.2012

    • Author(s)
      Yamamoto E, Suzuki H, Yamano HO
    • Journal Title

      Am J Pathol

      Volume: 181(5) Issue: 5 Pages: 1847-61

    • DOI

      10.1016/j.ajpath.2012.08.007

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] DGKGによる悪性黒色腫の浸潤抑制機構の解析2013

    • Author(s)
      佐藤亜紀子、甲斐正広、山本英一郎、丸山玲緒、西坂尚弘、山下利春、鈴木拓
    • Organizer
      第7回日本エピジェネティクス研究会総会
    • Place of Presentation
      奈良
    • Year and Date
      2013-05-30
    • Related Report
      2013 Final Research Report
  • [Presentation] DGK-γは黒色腫細胞の浸潤に関与する2012

    • Author(s)
      佐藤亜紀子、甲斐正広、鈴木拓、西坂尚弘、肥田時征、米田明弘、山下利春
    • Organizer
      日本研究皮膚科学会第37回年次学術大会・総会
    • Place of Presentation
      沖縄
    • Year and Date
      2012-12-07
    • Related Report
      2013 Final Research Report
  • [Presentation] DGK-γは黒色腫細胞の浸潤に関与する2012

    • Author(s)
      佐藤亜紀子、甲斐正広、鈴木拓、山本英一郎、丸山玲緒、西坂尚弘、山下利春
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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