| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
In this study, we evaluated a novel synthetic approach for a positron emitter labeled protein by cell-free protein synthesis (CFPS) system. Synthesis of ^<11>C-GFP using ^<11>C-Met and CFPS afforded the product within a short time. On the other hand, when using ^<18>F-Pro, the ^<18>F-labeled GFP was produced too slowly to use it practically. We succeeded to prepare^<11>C-labeled scFv antibody to EGFR, which showed specific binding to EGFR-positive tumorcells in vitro and specific uptake by the EGFR-positive tumor in vivo. These results suggest the usefulness of the ^<11>C-labeld protein synthesis by CFPS.
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