Elucidation of division control and aging mechanism in single mesenchymal stem cell.

• Project/Area Number: 23791446
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Keio University
• Principal Investigator: MABUCHI Yo 慶應義塾大学, 医学部, 共同研究員 (50424172)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 放射線治療生物学 / 間葉系幹細胞 / 単一分離 / 幹細胞老化 / Single cell sorting / 細胞老化

Research Abstract

Mesenchymal stem cells (MSCs), conventionally isolated based on their adherence to plastic are heterogeneous and have poor growth and differentiation, limiting the ability to investigate their intrinsic characteristics. We elucidate the division control a

Research Products

• [Journal Article] Mesenchymal stem cells regulate epithelial-mesenchymal transition and tumor progression of pancreatic cancer cells (2013)
  • Author(s): Ayano Kabashima-Niibe
  • Journal Title: Cancer Science Volume: 104 Issue: 2 Pages: 157-164
  • DOI: 10.1111/cas.12059
  • Peer Reviewed
• [Journal Article] Primary evaluation of induced pluripotent stem cells using flow cytometry (2013)
  • Author(s): Araki D, Kawamura Y, Niibe K, Suzuki S, Morikawa S, Mabuchi Y, Nakagawa T, Okano H and Matsuzaki Y.
  • Journal Title: Inflammation and Regeneration Volume: 33(1) Pages: 3-12
  • NAID: 130004943826
  • Peer Reviewed
• [Journal Article] Primary evaluation of induced pluripotent stem cells using flow cytometry. (2013)
  • Author(s): Araki D
  • Journal Title: Inflammation and Regeneration Volume: Vol.33 No.1 Pages: 3-12
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells regulate epithelial-mesenchymal transition and tumor progression of pancreatic cancer cells. (2013)
  • Author(s): Kabashima-Niibe A
  • Journal Title: Cancer Sci. Volume: 104(2) Pages: 157-164
  • Peer Reviewed
• [Journal Article] Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α (2012)
  • Author(s): Houlihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, Okano H, Matsuzaki Y.(These authors contributed equally to this work.)
  • Journal Title: Nat Protoc Volume: 7(12) Issue: 12 Pages: 2103-2111
  • DOI: 10.1038/nprot.2012.125
  • Peer Reviewed
• [Journal Article] Discovering the true identity and function of mesenchymal stem cells (2012)
  • Author(s): Mabuchi Y, Houlihan DD, Okano H, Matsuzaki Y.
  • Journal Title: Inflammation and Regeneration Volume: 32(4) Pages: 146-151
  • NAID: 130004943820
  • Peer Reviewed
• [Journal Article] Transplantation of side population cells restores the function of damaged exocrine glands through clusterin (2012)
  • Author(s): Mishima K., Inoue H., Nishiyama T., Mabuchi Y., Amano Y., Ide F., Matsui M., Yamada H., Yamamoto G., Tanaka J., Yasuhara R., Sakurai T., Lee M., Chiba K., Sumimoto H., Kawakami Y., Matsuzaki Y., Tsubota K., Saito I
  • Journal Title: Stem Cells Volume: 30 Issue: 9 Pages: 1925-37
  • DOI: 10.1002/stem.1173
  • Peer Reviewed
• [Journal Article] RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI(3) Kinase/Akt Signaling Pathways (2012)
  • Author(s): Muto J, Imai T, Ogawa D, Nishimoto Y, Okada Y, Mabuchi Y, Kawase T, Iwanami A, Mischel PS, Saya H, Yoshida K, Matsuzaki Y, Okano H
  • Journal Title: PLoS One Volume: 7(3) Issue: 3 Pages: e33431-e33431
  • DOI: 10.1371/journal.pone.0033431
  • Peer Reviewed
• [Journal Article] Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α. (2012)
  • Author(s): Houlihan DD and Mabuchi Y
  • Journal Title: Nat Protoc. Volume: 7(12) Pages: 2103-2111
  • Peer Reviewed
• [Journal Article] Discovering the true identity and function of mesenchymal stem cells. (2012)
  • Author(s): Mabuchi Y
  • Journal Title: Inflammation and Regeneration Volume: Vol.32 No.4 Pages: 146-151
  • NAID: 130004943820
  • Peer Reviewed
• [Journal Article] Transplantation of side population cells restores the function of damaged exocrine glands through clusterin. (2012)
  • Author(s): Mishima K
  • Journal Title: Stem cells Volume: 30(9) Pages: 1925-1937
  • Peer Reviewed
• [Journal Article] RNA-binding protein Musashi1 modulates glioma cell growth through the post-transcriptional regulation of Notch and PI3 kinase/Akt signaling pathways. (2012)
  • Author(s): Muto J
  • Journal Title: PLoS ONE Volume: 7(3)
  • Peer Reviewed
• [Journal Article] RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI3 Kinase/Akt Signaling Pathways (2012)
  • Author(s): Jun Muto, Takao Imai, Daisuke Ogawa, Yoshinori Nishimoto, Yohei Okada, Yo Mabuchi et al.
  • Journal Title: PLoS One Volume: 7 (3)
  • Peer Reviewed
• [Journal Article] Beneficial compaction of spinal cord lesion by migrating astrocytes through Glycogen Synthase Kinase-3 inhibition (2011)
  • Author(s): Renault-Mihara F, Katoh H, Ikegami T, Iwanami A, Mukaino M, Tada H, Yasuda A, Nori S, Shibata S, Saito K, Matsushita M, Kaibuchi K, Okada S, Toyama Y, Nakamura M, and Okano H
  • Journal Title: EMBO Mol Med Volume: 3 Issue: 11 Pages: 682-96
  • DOI: 10.1002/emmm.201100179
  • Peer Reviewed
• [Journal Article] Purified Mesenchymal Stem Cells Are an Efficient Source for iPS Cell Induction (2011)
  • Author(s): Niibe K
  • Journal Title: PLoS One Volume: 6 Issue: 3 Pages: e17610-e17610
  • DOI: 10.1371/journal.pone.0017610
  • Peer Reviewed
• [Journal Article] Beneficial compaction of spinal cord lesion by migrating astrocytes through glycogen synthase kinase-3 inhibition. (2011)
  • Author(s): Renault-Mihara F, Katoh H, Ikegami T, Iwanami A, Mukaino M, Yasuda A, Nori S, Mabuchi Y, Tada H, Shibata S, Saito K, Matsushita M, Kaibuchi K, Okada S, Toyama Y, Nakamura M, Okano H.
  • Journal Title: EMBO Mol Med Volume: 3 (11) Pages: 682-96
  • Peer Reviewed
• [Journal Article] Purified mesenchymal stem cells are an efficient source for iPS cell induction. (2011)
  • Author(s): Kunimichi Niibe, Yoshimi Kawamura, Daisuke Araki, Satoru Morikawa, Kyoko Miura, Sadafumi Suzuki, Shigeto Shimmura, Takehiko Sunabori, Yo Mabuchi et al.
  • Journal Title: Plos ONE Volume: 6(3)
  • Peer Reviewed
• [Journal Article] Schwann Cell Plasticity After Spinal Cord Injury Shown by Neural Crest Lineage Tracing. (2011)
  • Author(s): Nagoshi N, Shibata S, Nakamura M, Mabuchi Y, Matsuzaki Y, Toyama Y, and Okano H
  • Journal Title: Glia Volume: 59(5) Pages: 771-84
  • Peer Reviewed
• [Presentation] LNGFR+Thy-1+CD106high+ cells reveal functionally distinct subpopulations in human mesenchymal stem cells (2013)
  • Author(s): 馬渕洋
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 神奈川
• [Presentation] Identification of selective markers for highly purified human mesenchymal stem/stromal cells (2012)
  • Author(s): Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Francois R. M., Diarmaid D. H, Okano H & Matsuzaki Y
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-13
• [Presentation] Identification of selective markers for highly purified human mesenchymal stem/stromal cells. (2012)
  • Author(s): Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Francois R. M., Diarmaid D. H, Akazawa C, Okano H & Matsuzaki Y
  • Organizer: G-COE Final Symposium
  • Place of Presentation: 東京
  • Year and Date: 2012-12-04
• [Presentation] ヒト間葉系幹細胞の予期的分離と機能的解析 (2012)
  • Author(s): 馬渕洋、岡野栄之、松崎有未
  • Organizer: 第33回日本炎症・再生医学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-07-05
• [Presentation] LNGFR and THY-1 based prospective isolation of human mesenchymal stem cells reveals functionally distinct subpopulations. (2012)
  • Author(s): Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Sato Y, Francois R. M., Diarmaid D. H, Okano H & Matsuzaki Y.
  • Organizer: International Society for Stem Cell Research (ISSCR), 10th Annual Meeting
  • Place of Presentation: Yokohama
• [Presentation] Identification of selective markers for highly purified human mesenchymal stem cells (2012)
  • Author(s): 馬渕洋
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] ヒト間葉系幹細胞の予期的分離と機能的解析 (2012)
  • Author(s): 馬渕洋
  • Organizer: 第33回炎症再生医学会
  • Place of Presentation: 福岡
• [Presentation] LNGFR and Thy-1 Based prospective isolation of human Mesenchymal stem cells reveals functionally distinct subpopulations. (2012)
  • Author(s): Mabuchi Y
  • Organizer: 11th ISSCR Annual Meeting
  • Place of Presentation: 神奈川
• [Presentation] Single cell isolation elucidates heterogeneity within the multi-potent stem cell compartment. (2011)
  • Author(s): Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Okano H, Matsuzaki Y
  • Organizer: International Society for Stem Cell Research (ISSCR), 9th Annual Meeting
  • Place of Presentation: Canada
• [Presentation] DYNAMICS OF DELAYED P53 MUTATIONS IN MICE GIVEN WHOLE-BODY IRRADIATION AT EIGHT WEEKS (2011)
  • Author(s): Okazaki R, Ootsuyama A, Mabuchi Y, Matsuzaki Y, Michikawa Y, Imai T
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Poland
• [Presentation] PURIFIED MESENCHYMAL STEM CELLS ARE AN EFFICIENT CELL SOURCE FOR IPS CELLS INDUCTION (2011)
  • Author(s): Niibe, Kunimichi, Kawamura, Yoshimi, Morikawa, Satoru, Mabuchi, Yo, Araki, Daisuke, Nakagawa, Taneaki, Okano, Hideyuki, Matsuzaki, Yumi
  • Organizer: International Society for Stem Cell Research (ISSCR), 9th ANNUAL MEETING
  • Place of Presentation: Canada
• [Presentation] SINGLE CELL ISOLATION ELUCIDATES HETEROGENEITY WITHIN THE HUMAN MESENCHYMAL STEM/PROGENITOR CELL COMPARTMENT (2011)
  • Author(s): Mabuchi, Yo, Morikawa, Satoru, Harada, Seiko, Niibe, Kunimichi, Suzuki, Sadafumi, Okano, Hideyuki, Matsuzaki, Yumi
  • Organizer: International Society for Stem Cell Research (ISSCR), 9th ANNUAL MEETING
  • Place of Presentation: Canada
• [Remarks]
  • URL: http://www.okano-lab.com/publication
• [Remarks] 慶應義塾大学医学部生理学教室HP
  • URL: http://www.okano-lab.com/
• [Remarks]
  • URL: http://www.okano-lab.com/