| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
The aim of the present study was to identify subpopulation of stromal cells that suppresspancreatic cancer cells. We analyzed surface marker of pancreatic stellate cells(PSCs) derived from human pancreatic ductal adenocarcinoma(PDAC), and characterized CD271+ PSCs.Quantitative RT-PCR analyses revealed that CD271 mRNA expression was increased in PSCs after coculture with pancreatic cancer cells. However, the level of CD271 mRNA expressionsubsequently decreased after the transient increase. Furthermore, CD271 mRNA expression was decreased in PSCs migrating toward pancreatic cancer cells through Matrigel.Immunohistochemistry of PDAC revealed stromal CD271 high expression was associated with a good prognosis. These findings suggest that CD271+ PSCs appear at the early stage ofpancreatic carcinogenesis and have suppressive role against pancreatic cancer cells through cancer-stromal interaction
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