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Establishment of the high sensitive assay for detecting malignant pleural mesothelioma-specific DNA methylation

Research Project

Project/Area Number 23791570
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Thoracic surgery
Research InstitutionOkayama University

Principal Investigator

ASANO HIROAKI  岡山大学, 大学病院, 助教 (70534775)

Research Collaborator TOYOOKA Shinichi  岡山大学, 医歯薬学総合研究科, 教授 (30397880)
TSUKUDA Kazunori  岡山大学, 大学病院, 講師 (20346430)
SOH Junichi  岡山大学, 大学病院, 助教 (90559890)
MURAOKA Takayuki  岡山大学, 大学病院, 医員(現在:屋島総合病院・外科)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords胸膜悪性中皮腫 / マイクロRNA / miR-34b/c / DNAメチル化 / デジタルPCR / 血清DNA / 悪性中皮腫 / 早期診断
Research Abstract

We established a high sensitivity assay for detecting microRNA(hsa-miR-34b/c) methylation in serum-circulating DNA from patients with malignant pleural mesothelioma using digital methylation-specific PCR. The specificity of this assay was 77 % and sensitivity was 67%. Further investigation is required for improvement of both sensitivity and specificity by combining with other biomarkers for clinical application.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (2 results)

All 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] The degree of microRNA-34b/c methylation in serum-circulating DNA is associated with malignant pleural mesothelioma.2013

    • Author(s)
      Muraoka T, Soh J, Toyooka S, Aoe K, Fujimoto N, Hashida S, Maki Y, Tanaka N, Shien K, Furukawa M, Yamamoto H, Asano H, Tsukuda K, Kishimoto T, Otsuki T, Miyoshi S.
    • Journal Title

      Lung Cancer

      Volume: 82 Issue: 3 Pages: 485-490

    • DOI

      10.1016/j.lungcan.2013.09.017

    • NAID

      120005367626

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Usefulness of sensitive digital PCR assay to quantify microRNA-34b/c methylation in the circulating serum DNA of malignant mesothelioma patients2012

    • Author(s)
      Takayuki Muraoka
    • Organizer
      American Association for Cancer Research Annual Meeting
    • Place of Presentation
      米国
    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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