Establishment of the high sensitive assay for detecting malignant pleural mesothelioma-specific DNA methylation
| Project/Area Number |
23791570
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Thoracic surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Research Collaborator |
TOYOOKA Shinichi 岡山大学, 医歯薬学総合研究科, 教授 (30397880)
TSUKUDA Kazunori 岡山大学, 大学病院, 講師 (20346430)
SOH Junichi 岡山大学, 大学病院, 助教 (90559890)
MURAOKA Takayuki 岡山大学, 大学病院, 医員(現在:屋島総合病院・外科)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 胸膜悪性中皮腫 / マイクロRNA / miR-34b/c / DNAメチル化 / デジタルPCR / 血清DNA / 悪性中皮腫 / 早期診断 |
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| Research Abstract |
We established a high sensitivity assay for detecting microRNA(hsa-miR-34b/c) methylation in serum-circulating DNA from patients with malignant pleural mesothelioma using digital methylation-specific PCR. The specificity of this assay was 77 % and sensitivity was 67%. Further investigation is required for improvement of both sensitivity and specificity by combining with other biomarkers for clinical application.
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Report
(4 results)
Research Products
(2 results)
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[Journal Article] The degree of microRNA-34b/c methylation in serum-circulating DNA is associated with malignant pleural mesothelioma.2013
Author(s)
Muraoka T, Soh J, Toyooka S, Aoe K, Fujimoto N, Hashida S, Maki Y, Tanaka N, Shien K, Furukawa M, Yamamoto H, Asano H, Tsukuda K, Kishimoto T, Otsuki T, Miyoshi S.
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Journal Title
Lung Cancer
Volume: 82
Issue: 3
Pages: 485-490
DOI
NAID
Related Report
Peer Reviewed
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