Identifying challenges to discover cancer stem-like cells in osteosarcoma
| Project/Area Number |
23791629
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Yasuhiko 金沢大学, 医学系, 准教授 (20313637)
MUNESUE Seiichi 金沢大学, 医学系, 助教 (10399040)
HARASHIMA Ai 金沢大学, 脳・肝インターフェースメディシン研究センター, 助教 (50705522)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 骨・軟部腫瘍学 / 骨肉腫 / 幹細胞 / 糖化蛋白受容体 |
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| Research Abstract |
Induced expression of RAGE (receptor for advanced glycation end-products) resulted in the co-expression of CD133 and CD24, stem-cell markers, in human osteosarcoma cell lines. Moreover, the RAGE-overexpressing cells showed higher spheroid-forming ability and a resistance to treatment of cisplatin, an anti-cancer drug, in vitro.Upregulated gene expression of cell-cycle and cell-division drivers was observed in microarray analyses . We also found that the expression of CD133 was significantly correlated with poor prognosis in patients with osteosarcoma using immunohistochemical analyses. Our results suggest that the RAGE could elicit the expression of cancer stem cell markers and may promote the cancer stem-like cell characteristics in osteosarcoma.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Prognostic value of histological response to chemotherapy in osteosarcoma patients receiving tumor-bearing frozen autograft2013
Author(s)
Miwa S, Takeuchi A, Ikeda H, Shirai T, Yamamoto N, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Igarashi K, Tsuchiya H
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Journal Title
PLoS One
Volume: 8(8)
Issue: 8
Pages: 71362-71362
DOI
Related Report
Peer Reviewed
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[Journal Article] Pedicle versus free frozen autograft for reconstruction in malignant bone and soft tissue tumors of the lower extremities2013
Author(s)
Shimozaki S, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Kato T, Tsuchiya H
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Journal Title
J Orthop Sci
Volume: 19(1)
Issue: 1
Pages: 156-63
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] A novel combined radiological method for evaluation of the response to chemotherapy for primary bone sarcoma2012
Author(s)
Miwa S, Taki J, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Ooi A, Tsuchiya H
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Journal Title
Journal of Surgical Oncology
Volume: 106
Issue: 3
Pages: 273-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways2012
Author(s)
Miwa S, Sugimoto N, Yamamoto N, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Yachie A, Tsuchiya H
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Journal Title
Anticancer Res
Volume: 32
Pages: 3643-9
URL
Related Report
Peer Reviewed
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[Presentation] 骨肉腫に対するカフェイン併用化学療法と縮小手術の中長期成績2013
Author(s)
武内章彦,山本憲男,白井寿治,西田英司,林克洋,丹沢義一,木村浩明,五十嵐健太郎,稲谷弘幸,下崎真吾,加藤貴士,青木裕,土屋弘行
Organizer
第51回日本癌治療学会学術集会
Place of Presentation
国立京都国際会館
Related Report
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