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Identifying challenges to discover cancer stem-like cells in osteosarcoma

Research Project

Project/Area Number 23791629
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionKanazawa University

Principal Investigator

TAKEUCHI Akihiko  金沢大学, 大学病院, 助教 (70512218)

Co-Investigator(Renkei-kenkyūsha) YAMAMOTO Yasuhiko  金沢大学, 医学系, 准教授 (20313637)
MUNESUE Seiichi  金沢大学, 医学系, 助教 (10399040)
HARASHIMA Ai  金沢大学, 脳・肝インターフェースメディシン研究センター, 助教 (50705522)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords骨・軟部腫瘍学 / 骨肉腫 / 幹細胞 / 糖化蛋白受容体
Research Abstract

Induced expression of RAGE (receptor for advanced glycation end-products) resulted in the co-expression of CD133 and CD24, stem-cell markers, in human osteosarcoma cell lines. Moreover, the RAGE-overexpressing cells showed higher spheroid-forming ability and a resistance to treatment of cisplatin, an anti-cancer drug, in vitro.Upregulated gene expression of cell-cycle and cell-division drivers was observed in microarray analyses . We also found that the expression of CD133 was significantly correlated with poor prognosis in patients with osteosarcoma using immunohistochemical analyses. Our results suggest that the RAGE could elicit the expression of cancer stem cell markers and may promote the cancer stem-like cell characteristics in osteosarcoma.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report

Research Products

(39 results)

All 2014 2013 2012 2011

All Journal Article (19 results) (of which Peer Reviewed: 18 results) Presentation (14 results) Patent(Industrial Property Rights) (6 results) (of which Overseas: 2 results)

  • [Journal Article] Pedicle versus free frozen autograft for reconstruction in malignant bone and soft tissue tumors of the lower extremities2014

    • Author(s)
      Shimozaki S, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Kato T, Tsuchiya H
    • Journal Title

      J Orthop Sci.

      Volume: 19(1) Pages: 156-163

    • DOI

      10.1007/s00776-013-0487-x

    • NAID

      120005349170

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic value of histological response to chemotherapy in osteosarcoma patients receiving tumor-bearing frozen autograft2013

    • Author(s)
      Miwa S, Takeuchi A, Ikeda H, Shirai T, Yamamoto N, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Igarashi K, Tsuchiya H
    • Journal Title

      PLoS One

      Volume: 8(8) Pages: 71362-71362

    • DOI

      10.1371/journal.pone.0071362

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic value of radiological response to chemotherapy in patients with osteosarcoma.2013

    • Author(s)
      Miwa S
    • Journal Title

      PLoS One

      Volume: 8(7)

    • DOI

      10.1371/journal.pone.0070015

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] H. Complete necrosis of a giant cell tumor with high expression of PPARγ : a case report2013

    • Author(s)
      Takeuchi A, Yamamoto N, Nishida H, Kimura H, Ikeda H, Tsuchiya H
    • Journal Title

      Anticancer Res

      Volume: 33 Pages: 2169-74

    • URL

      http://ar.iiarjournals.org/content/33/5/2169

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low molecular weight heparin suppresses receptor for advanced glycation end products-mediated expression of malignant phenotype in human fibrosarcoma cells.2013

    • Author(s)
      Takeuchi A, Yamamoto Y, Munesue S, Harashima A, Watanabe T, Yonekura H, Yamamoto H, Tsuchiya H.
    • Journal Title

      Cancer Sci

      Volume: 104(6) Pages: 1-10

    • DOI

      10.1111/cas.12133

    • NAID

      120005830605

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Uncultured adipose-derived regenerative cells promote peripheral nerve regeneration2013

    • Author(s)
      Suganuma S, Tada K, Hayashi K, Takeuchi A, Sugimoto N, Ikeda K, Tsuchiya H
    • Journal Title

      J Orthop Sci.

      Volume: 18 Pages: 145-151

    • DOI

      10.1007/s00776-012-0306-9

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Over 10-year follow-up of functional outcome in patients with bone tumors reconstructed using distraction osteogenesis.2013

    • Author(s)
      Watanabe K
    • Journal Title

      J Orthop Sci.

      Volume: 18(1) Pages: 101-109

    • DOI

      10.1007/s00776-012-0327-4

    • NAID

      120004966628

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fifteen-year survivor of renal cell carcinoma after metastasectomies for multiple bone metastases2013

    • Author(s)
      Kato S, Murakami H, Takeuchi A, Demura S, Yoshioka K, Kawahara N, Tomita K, Tsuchiya H
    • Journal Title

      Orthopedics

      Volume: 36

    • DOI

      10.3928/01477447-20131021-32

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors2013

    • Author(s)
      Miwa S, Nishida H, Tanzawa Y, Takeuchi A, Yamamoto N, Hayashi K, Kimura H, Tsuchiya H
    • Journal Title

      PLoS One

      Volume: 926

    • DOI

      10.1371/annotation/7c0f14e6-cfba-4f5f-b93d-1a149eaeec96

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic value of histological response to chemotherapy in osteosarcoma patients receiving tumor-bearing frozen autograft.2013

    • Author(s)
      Miwa S
    • Journal Title

      PLoS One

      Volume: 8(8)

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete necrosis of a giant cell tumor with high expression of PPARγ: a case report.2013

    • Author(s)
      Takeuchi A
    • Journal Title

      Anticancer Res.

      Volume: 33(5) Pages: 2169-74

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete Necrosis of Giant Cell Tumor with High Expression of PPARγ: A Case Report2013

    • Author(s)
      Takeuchi A
    • Journal Title

      Anticancer Research

      Volume: In press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors2013

    • Author(s)
      Miwa S, Tsuchiya H, et al
    • Journal Title

      PloS One

      Volume: 7

    • DOI

      10.1371/journal.pone.0052926

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Treatment strategies for well-differentiated liposarcomas and therapeutic outcomes2012

    • Author(s)
      Yamamoto N, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kitamura S, Tsuchiya H
    • Journal Title

      Anticancer Res

      Volume: 32 Pages: 1821-5

    • NAID

      120004442385

    • URL

      http://ar.iiarjournals.org/content/32/5/1821

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel combined radiological method for evaluation of the response to chemotherapy for primary bone sarcoma2012

    • Author(s)
      Miwa S, Taki J, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Ooi A, Tsuchiya H
    • Journal Title

      J Surg Oncol

      Volume: 106 Pages: 273-279

    • DOI

      10.1002/jso.23074

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways2012

    • Author(s)
      Miwa S, Sugimoto N, Yamamoto N, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Yachie A, Tsuchiya H
    • Journal Title

      Anticancer Res

      Volume: 32 Pages: 3643-9

    • URL

      http://ar.iiarjournals.org/content/32/9/3643

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways.2012

    • Author(s)
      Miwa S
    • Journal Title

      Anticancer Res

      Volume: 32(9) Pages: 3642-3649

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Treatment strategies for well-differentiated liposarcomas and therapeutic outcomes.2012

    • Author(s)
      Yamamoto N
    • Journal Title

      Anticancer Res

      Volume: 32(5) Pages: 1821-1825

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] がん幹細胞2011

    • Author(s)
      武内章彦、土屋弘行
    • Journal Title

      臨床整形外科

      Volume: 46 Pages: 1022-1026

    • Related Report
      2011 Research-status Report
  • [Presentation] 骨肉腫に対するカフェイン併用化学療法と縮小手術2013

    • Author(s)
      武内章彦,山本憲男,白井寿治,林克洋,西田英司,丹澤義一,木村浩明,三輪真嗣,土屋弘行
    • Organizer
      第86回日本整形外科学会学術集会
    • Place of Presentation
      リーガロイヤルホテル広島
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨巨細胞腫に対するペルオキシゾーム増殖剤応答性受容体ガンマ活性化による分化誘導及び抗腫瘍効果の解析2013

    • Author(s)
      武内章彦,山本憲男,西田英司,木村浩明,五十嵐健太郎,下崎真吾,加藤貴士,田中一広,山本靖彦,土屋弘行
    • Organizer
      第46回日本整形外科学会骨・軟部腫瘍学術集会
    • Place of Presentation
      東京ドームホテル
    • Related Report
      2013 Final Research Report
  • [Presentation] 粘液型脂肪肉腫におけるペルオキシゾーム増殖剤応答性受容体ガンマの発現と臨床経過との相関2013

    • Author(s)
      武内章彦,山本憲男,西田英司,木村浩明,五十嵐健太郎,下崎真吾,加藤貴士,土屋弘行
    • Organizer
      第46回日本整形外科学会骨・軟部腫瘍学術集会
    • Place of Presentation
      東京ドームホテル
    • Related Report
      2013 Final Research Report
  • [Presentation] 低分子ヘパリンは線維肉腫細胞における後期糖化産物受容体(RAGE)を介する悪性度を抑制する2013

    • Author(s)
      武内章彦,山本靖彦,棟居聖一,原島愛,山本憲男,土屋弘行
    • Organizer
      第72回日本癌学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] Zaltoprofenは骨巨細胞腫に対してPPARγ(peroxisome proliferator-activated receptorγ)の活性化により抗腫瘍効果および脂肪分化誘導を示す2013

    • Author(s)
      武内章彦,山本憲男,西田英司,木村浩明,下崎真吾,加藤貴士,山本靖彦,土屋弘行
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      幕張メッセ
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨肉腫に対するカフェイン併用化学療法と縮小手術の中長期成績2013

    • Author(s)
      武内章彦,山本憲男,白井寿治,西田英司,林克洋,丹沢義一,木村浩明,五十嵐健太郎,稲谷弘幸,下崎真吾,加藤貴士,青木裕,土屋弘行
    • Organizer
      第51回日本癌治療学会学術集会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨肉腫に対するカフェイン併用化学療法と縮小手術2013

    • Author(s)
      武内 章彦
    • Organizer
      第86回日本整形外科学会学術集会
    • Place of Presentation
      リーガロイヤルホテル広島(広島)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 骨巨細胞腫に対するペルオキシゾーム増殖剤応答性受容体ガンマ活性化による分化誘導及び抗腫瘍効果の解析2013

    • Author(s)
      武内 章彦
    • Organizer
      第46回日本整形外科学会 骨・軟部腫瘍学術集会
    • Place of Presentation
      東京ドームホテル(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 粘液型脂肪肉腫におけるペルオキシゾーム増殖剤応答性受容体ガンマの発現と臨床経過との相関2013

    • Author(s)
      武内 章彦
    • Organizer
      第46回日本整形外科学会 骨・軟部腫瘍学術集会
    • Place of Presentation
      東京ドームホテル(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 低分子ヘパリンは線維肉腫細胞における後期糖化産物受容体(RAGE)を介する悪性度を抑制する2013

    • Author(s)
      武内 章彦
    • Organizer
      第72回日本癌学会学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Zaltoprofen は 骨 巨 細 胞 腫 に 対 し て PPARγ (peroxisome proliferator-activated receptor γ) の活性化により抗腫瘍効果および脂肪分化誘導を示す2013

    • Author(s)
      武内 章彦
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 骨肉腫に対するカフェイン併用化学療法と縮小手術の中長期成績2013

    • Author(s)
      武内 章彦
    • Organizer
      第51回日本癌治療学会学術集会
    • Place of Presentation
      京都国際会館(京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 骨肉腫におけるがん幹細胞研究2012

    • Author(s)
      武内章彦,山本靖彦,山本博,土屋弘行
    • Organizer
      第45回日本整形外科学会骨・軟部腫瘍学術集会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨肉腫におけるがん幹細胞研究2012

    • Author(s)
      武内 章彦
    • Organizer
      第45回日本整形外科骨・軟部腫瘍学術集会
    • Place of Presentation
      東京都・東京国際フォーラム
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 骨・軟部に発生する巨細胞性腫瘍または軟骨肉腫の予防または治療のための薬剤、動脈塞栓術用局所注入材、および人工骨2013

    • Inventor(s)
      武内章彦、土屋弘行
    • Industrial Property Rights Holder
      武内章彦、土屋弘行
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-199076
    • Filing Date
      2013-09-25
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 骨・軟部に発生する巨細胞性腫瘍の予防または治療剤2013

    • Inventor(s)
      武内章彦、土屋弘行
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-03-18
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 骨・軟部に発生する巨細胞性腫瘍または軟骨肉腫の予防または治療のため の薬剤、動脈塞栓術用局所注入剤、および、人工骨2013

    • Inventor(s)
      武内章彦、土屋弘行
    • Industrial Property Rights Holder
      武内章彦、土屋弘行
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-199076
    • Filing Date
      2013-09-25
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 骨・軟部に発生する巨細胞性腫瘍の予防または治療剤2013

    • Inventor(s)
      武内章彦 土屋弘行
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-03-18
    • Related Report
      2012 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 骨・軟部に発生する巨細胞性腫瘍の予防または治療剤2012

    • Inventor(s)
      武内章彦、土屋弘行
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-235784
    • Filing Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 骨・軟部に発生する巨細胞性腫瘍の予防または治療剤2012

    • Inventor(s)
      武内章彦 土屋弘行
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-235784
    • Filing Date
      2012-10-25
    • Related Report
      2012 Research-status Report

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Published: 2011-08-04   Modified: 2019-07-29  

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