Molecular profiling to predict chemosensitivity for osteosarcoma

• Project/Area Number: 23791673
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: IWATA Shintaro 千葉県がんセンター(研究所), 医療局 整形外科, 主任医長 (90549685)
• Co-Investigator(Renkei-kenkyūsha): 大平 美紀 千葉県がんセンター研究所, がんゲノム研究室長 (20311384)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 骨肉腫 / CGH / 薬剤感受性 / ゲノム / 次世代シーケンサー / ゲノム解析 / 次世代シーケンシング / アレイCGH

Research Abstract

Chemosensitivity is the most important prognostic factor for osteosarcoma (OS). We have carried out our project to identify possible genomic markers for the prediction of chemosensitivity for pediatric OS patients using CGH technique. 22 tumor samples of pediatric conventional OS patients who divided into three groups according to the histological response to chemotherapy were applied.
Differential aberration analyses resulted that 8 putative genomic markers harboring OS-related genes were selected for classifying each 3 groups. Scoring system to predict chemosensitivity was developed with these genomic markers and validation analysis with 8 independent patients confirmed that the scoring system was remarkably consistent to the patient properties. These signatures may be useful for the prediction of chemosensitivity and validation with more samples is awaited.

Research Products

• [Journal Article] Prognostic Factors in eElderly Osteosarcoma Patients : A Multi-institutional Retrospective Study of 86 Cases (2014)
  • Author(s): Iwata S, Ishii T, Kawai A, Hiruma T, Yonemoto T, Kamoda H, Asano N, Takeyama M
  • Journal Title: Ann Surg Oncol Volume: 21 Pages: 263-268
• [Journal Article] Prognostic Factors in Elderly Osteosarcoma Patients: A Multi-institutional Retrospective Study of 86 Cases. (2014)
  • Author(s): Iwata S, Ishii T, Kawai A, Hiruma T, Yonemoto T, Kamoda H, Asano N, Takeyama M.
  • Journal Title: Ann Surg Oncol Volume: 21 Issue: 1 Pages: 263-268
  • DOI: 10.1245/s10434-013-3210-4
  • Peer Reviewed / Open Access
• [Presentation] ゲノム解析による小児骨肉腫患者の至適化学療法予測システム (2013)
  • Author(s): 岩田慎太郎、大平美紀、石井猛、影山肇、片山稔、横井左奈、永瀬浩喜、米本司、中川原章
  • Organizer: 第72回日本癌学会総会
  • Place of Presentation: 横浜
• [Presentation] ゲノム解析の手法を用いた小児四肢発生骨肉腫患者の至適化学療法予測システムの確立 (2013)
  • Author(s): 岩田慎太郎、大平美紀、石井猛、影山肇、片山稔、横井左奈、永瀬浩喜、米本司、中川原章
  • Organizer: 第51回日本癌治療学会学術集会
  • Place of Presentation: 京都
• [Presentation] Characterization of genomic alterations in pediatric osteosarcoma with differential chmosensitivities : Construction of genome-based prediction system using preoperative biopsy samples (2013)
  • Author(s): Iwata S, Yonemoto T, Kageyama H, Yokoi S, Nagase H, Nakagawara A, Ishii T, Ohira M
  • Organizer: AACR Special Conference on Pediatric Cancer at the Crossroads : Translating Discovery Into Improved Outcomes
  • Place of Presentation: San Diego, USA
• [Presentation] Genomic predictors of response to preoperative chemotherapy in patients with pediatric osteosarcoma (2013)
  • Author(s): Iwata S, Ohira M, Yonemoto T, Ishii T, Kamoda H, Nakagawara A.
  • Organizer: American Association for Cancer Research 104th Annual Meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] ゲノム解析による小児骨肉腫患者の至適化学療法予測システム (2013)
  • Author(s): 岩田慎太郎、大平美紀、石井 猛、影山 肇、片山 稔、横井左奈、永瀬浩喜、米本 司、中川原 章.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] ゲノム解析の手法を用いた小児四肢発生骨肉腫患者の至適化学療法予測システムの確立. (2013)
  • Author(s): 岩田慎太郎、大平美紀、米本 司、萩原洋子、永瀬浩喜、影山 肇、片山 稔、鴨田博人、横井左奈、石井 猛、中川原 章.
  • Organizer: 第51回日本癌治療学会学術集会
  • Place of Presentation: 京都
• [Presentation] Characterization of genomic alterations in pediatric osteosarcoma with differential chemosensitivities: Construction of genome-based prediction system using preoperative biopsy samples (2013)
  • Author(s): Iwata S, Yonemoto T, Kageyama H, Yokoi S, Nagase H, Nakagawara A, Ishii T, Ohira M
  • Organizer: AACR Special Conference on Pediatric Cancer at the Crossroads: Translating Discovery Into Improved Outcomes
  • Place of Presentation: San Diego, USA
• [Presentation] Genomic predictor of response to preoperative chemotherapy in patients with pediatric osteosarcoma (2012)
  • Author(s): Iwata S, Ohira M, Ishii T, Tatezaki S, Nakagawara A
  • Organizer: 2012 The Connective Tissue Oncology Society Annual Meeting
  • Place of Presentation: Prague, Czech
• [Presentation] ゲノム解析による骨肉腫患者の至適化学療法予測システム (2012)
  • Author(s): 岩田慎太郎大平美紀米本司石井猛鴨田博人中川原章
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Genomic approach to the prediction of chemotherapy sensitivity in osteosarcoma (2012)
  • Author(s): Iwata S, Ohira M, Kamijo T, Ishii T, Tatezaki S, Nakagawara A
  • Organizer: American Association for Cancer Research Annual Meeting 2012
  • Place of Presentation: Chicago, USA
• [Presentation] Genomic predictors of response to preoperative chemotherapy in patients with pediatric osteosarcoma (2012)
  • Author(s): Iwata S, Ohira M, Yonemoto T, Ishii T, Kamoda H, Nakagawara A
  • Organizer: American Association for Cancer Research Annual Meeting 2013
  • Place of Presentation: Washington DC, USA
• [Presentation] GENOMIC PREDICTOR OF RESPONSE TO PREOPERATIVE CHEMOTHERAPY IN PATIENTS WITH PEDIATRIC OSTEOSARCOMA (2012)
  • Author(s): Iwata S, Ohira M, Ishii T, Tatezaki S, Nakagawara A
  • Organizer: The Connective Tissue Oncology Society Annual Meeting
  • Place of Presentation: Prague, Czech
• [Presentation] Genomic approach to the prediction of chemotherapy sensitivity in osteosarcoma (2012)
  • Author(s): Iwata S, Ohira M, Kamijo T, Ishii T, Tatezaki S, Nakagawara A
  • Organizer: American Association for Cancer Research Annual Meeting
  • Place of Presentation: Chicago, USA
• [Presentation] Genomic approach to the prediction of chemotherapy sensitivity in osteosarcoma (2011)
  • Author(s): Iwata S, Ohira M, Ishii T, Yonemoto T, Hagiwara Y, Tatezaki S, Nakagawara A
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-05
• [Presentation] Genomic approachによる骨肉腫の化学療法感受性予測 (2011)
  • Author(s): 岩田慎太郎、大平美紀、上條岳彦、米本司、石井猛、舘崎愼一郎、中川原章
  • Organizer: 第44回日本整形外科学会骨・軟部腫瘍学術集会
  • Place of Presentation: 京都
  • Year and Date: 2011-07-14
• [Book] 5.骨肉腫におけるゲノム解析の現状と未来先端医療シリーズ44「臨床医のための最新整形外科」(平澤泰介) (2013)
  • Author(s): 岩田慎太郎、大平美紀、米本司
  • Publisher: 先端医療技術研究所
• [Book] 先端医療シリーズ 44 「臨床医のための最新整形外科」（分担執筆） (2013)
  • Author(s): 岩田慎太郎、大平美紀、米本司
  • Total Pages: 526
  • Publisher: 先端医療技術研究所