Effect of chondrogenic differentiation in mesenchymal stem cells on the stiffness of three dimensional extracellular matrix

• Project/Area Number: 23791678
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Metropolitan Industrial Technology Research Institute
• Principal Investigator: OHYABU Yoshimi 地方独立行政法人東京都立産業技術研究センター, 開発本部開発第二部バイオ応用技術グループ, 副主任研究員 (80587410)
• Project Period (FY): 2011-04-28 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 硬さ / 3次元 / ゼラチン / 線維化 / ３次元 / 幹細胞 / 分化 / 三次元 / 硬さ制御 / 分化制御

Outline of Final Research Achievements

Generically, we used crosslinker that has a cytotoxic to control stiffness and was not able to be cultured for the cells in three-dimensional. Gelatin uncleaved molecular chains (uncleaved gelatin; UCG) were tends to restore the helical structure at 30 degrees C and confirmed to form fibrils similar to the collagen fibers. We identified the fiber-forming component that is included in UCG and developed a method for fabrication of high-density collagen fibril matrix gels from concentrated solutions of UCG. High-strength CFM may provide more durable hydrogels for tissue engineering and regenerative medicine.

Research Products

• [Journal Article] Evaluation of gelatin hydrogel as a potential carrier for cell transportation (2014)
  • Author(s): Ohyabu Y, Hatayama H, Yunoki S.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 未定 Issue: 1 Pages: 112-115
  • DOI: 10.1016/j.jbiosc.2013.12.005
  • NAID: 110009838780
  • Peer Reviewed
• [Journal Article] Fabrication of high-density collagen fibril matrix gels by renaturation of triple-helix collagen from gelatin (2013)
  • Author(s): Ohyabu Y, Yunoki S, Hatayama H, Teranishi Y.
  • Journal Title: International Journal of Biological Macromolecules Volume: 62 Pages: 296-303
  • DOI: 10.1016/j.ijbiomac.2013.09.001
  • Peer Reviewed
• [Presentation] 高いゲル化温度を有するゼラチンを用いた細胞輸送用ゲルマトリックスの開発 (2014)
  • Author(s): 大藪淑美、畑山 博哉、柚木俊二
  • Organizer: 第１３回日本再生医療学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-03-05
• [Presentation] Preparation of high-density collagen matrix via denaturation and renaturation of collagen (2013)
  • Author(s): Yunoki S, Hatayama H, Ohyabu Y
  • Organizer: TERMIS-AP2013
  • Place of Presentation: Shanghai, China
  • Year and Date: 2013-10-26
• [Presentation] Evaluation of uncleaved gelatin as a potential carrier for cell transportation (2013)
  • Author(s): Hatayama H, Ohyabu Y, Yunoki S
  • Organizer: TERMIS-AP2013
  • Place of Presentation: Shanghai, China
  • Year and Date: 2013-10-24
• [Presentation] ゼラチンの３重らせん回復現象による高密度コラーゲン線維マトリックスゲルの作製 (2013)
  • Author(s): 柚木俊二、畑山 博哉、大藪 淑美
  • Organizer: 第６２回高分子討論会
  • Place of Presentation: 金沢
  • Year and Date: 2013-09-11
• [Presentation] 施設間細胞輸送用キャリアとしてのゼラチンハイドロゲルの可能性 (2013)
  • Author(s): 畑山 博哉、大藪 淑美、柚木俊二
  • Organizer: 第６２回高分子討論会
  • Place of Presentation: 金沢
  • Year and Date: 2013-09-11
• [Presentation] 細胞搬送用基材としても高融点ゼラチンの開発 (2012)
  • Author(s): 畑山博哉、大藪淑美、柚木俊二
  • Organizer: 日本バイオマテリアル学会シンポジウム2012
  • Place of Presentation: 仙台国際センター
• [Presentation] iPS細胞等幹細胞/フィーダー細胞の分離培養膜の開発 (2012)
  • Author(s): 大藪淑美、柚木俊二、畑山博哉
  • Organizer: 日本バイオマテリアル学会シンポジウム2012
  • Place of Presentation: 仙台国際センター
• [Patent(Industrial Property Rights)] 高融点ゼラチン組成物、その製造方法、およびその用途 (2012)
  • Inventor(s): 大藪淑美、柚木俊二、畑山博哉
  • Industrial Property Rights Holder: 大藪淑美、柚木俊二、畑山博哉
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-11-21