Effect of chondrogenic differentiation in mesenchymal stem cells on the stiffness of three dimensional extracellular matrix
Project/Area Number |
23791678
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Orthopaedic surgery
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Research Institution | Tokyo Metropolitan Industrial Technology Research Institute |
Principal Investigator |
OHYABU Yoshimi 地方独立行政法人東京都立産業技術研究センター, 開発本部開発第二部バイオ応用技術グループ, 副主任研究員 (80587410)
|
Project Period (FY) |
2011-04-28 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 硬さ / 3次元 / ゼラチン / 線維化 / 3次元 / 幹細胞 / 分化 / 三次元 / 硬さ制御 / 分化制御 |
Outline of Final Research Achievements |
Generically, we used crosslinker that has a cytotoxic to control stiffness and was not able to be cultured for the cells in three-dimensional. Gelatin uncleaved molecular chains (uncleaved gelatin; UCG) were tends to restore the helical structure at 30 degrees C and confirmed to form fibrils similar to the collagen fibers. We identified the fiber-forming component that is included in UCG and developed a method for fabrication of high-density collagen fibril matrix gels from concentrated solutions of UCG. High-strength CFM may provide more durable hydrogels for tissue engineering and regenerative medicine.
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Report
(4 results)
Research Products
(10 results)