A prediction and a therapeutic strategy for lung hypoplasia in a rat CDH model
| Project/Area Number |
23791826
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
TSUDA HIROYUKI 名古屋大学, 医学部附属病院, 助教 (40571328)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 横隔膜ヘルニア / 肺低形成 / ラメラ体 / 羊水 / 予防 / 柴苓湯 |
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| Research Abstract |
We initially enrolled 19 cases diagnosed with CDH prenatally. LBC was significantly higher in the surviving group than in deceased group (p < 0.01).In the rat experiment, we collected amniotic fluid and lung tissue from newborns at E21 and confirmed the presence of lamellar bodies in both the amniotic fluid. We also confirmed a similar lamellar body size in amniotic fluid and type II alveolar cells comparable to the lamellar body findings in human amniotic fluid. Next, after exposure of pregnant rats to a 100 mg dose of nitrofen, 48.8% (61/125) of newborns had diaphragmatic hernias. LBC was significantly higher in control than in CDH (p < 0.01) rats. Furthermore, lung weight was also significantly higher in control than in CDH (p < 0.01) newborn rats. We examined the expression of ABCA3 in lung tissues from newborn rats, delivered at E21, by Western blotting. ABCA3 expression did not differ significantly between controls and rats with CDH.
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Report
(4 results)
Research Products
(8 results)
