Study of auditory cortex function and frequency map in mice using flavoprotein fluorescence imaging

• Project/Area Number: 23791882
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Niigata University
• Principal Investigator: KUBOTA Yamato 新潟大学, 医歯学総合病院, 助教 (40547593)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 聴覚医学 / 中枢聴覚 / イメージング / 大脳聴覚野 / 周波数マップ / 中枢性耳鳴 / 耳鳴

Research Abstract

We studied the cerebral auditory cortex function in mice using flavoprotein fluorescence imaging method.As a result,we got the following findings.1:After continuous tonal sonud exposure,On and Off responces in A1 were strongly observed.2:Changing the direction of frequency modulation of stimuli,specific reactions were observed at UF and DP area.It had been projected from the site that is different from the primary auditory cortex in the medial geniculate body.3:Age-related hearing loss mouse,there was no apparent change in the auditory cortex map in moderate hearing loss.Activities in midrange stimuli had become strong when it comes to severe hearing loss.4:In both sides measurement,strong responces were observed in the contralateral auditory cortex of the stimulus side in the auditory cortex.

Research Products

• [Journal Article] Auditory cortical areas activated by slow frequency-modulated sounds in mice (2013)
  • Author(s): Honma Y, Tsukano H, Horie M, Ohshima S, Tohmi M, Kubota Y, Takahashi K, Hishida R, Takahashi S, Shibuki K
  • Journal Title: PLoS One Volume: 8(7)
  • Peer Reviewed
• [Presentation] マウス聴覚・視覚連想記憶のM字迷路による解析 (2014)
  • Author(s): 山岸達也、塚野浩明、馬場洋徳、本間悠介、大島伸介、窪田和、高橋邦行、髙橋姿、澁木克栄
  • Organizer: 第91回日本生理学大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
  • Year and Date: 2014-03-16
• [Presentation] マウス聴覚・視覚連想記憶のＭ字迷路による解析 (2014)
  • Author(s): 山岸達也、塚野浩明、馬場洋徳、本間悠介、大島伸介、窪田和、高橋邦行、髙橋姿、澁木克栄
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学
• [Presentation] マウス聴覚・視覚連想記憶のM字迷路による解析 (2013)
  • Author(s): 山岸達也、馬場洋徳、本間悠介、大島伸介、窪田和、高橋邦行、澁木克栄、髙橋姿
  • Organizer: 第18回聴覚研究フォーラム
  • Place of Presentation: 同志社びわこリトリートセンター
  • Year and Date: 2013-12-04
• [Presentation] Cortical depression in the mouse auditory cortex after sound discrimination learning (2013)
  • Author(s): S.Ohshima, K.Takahashi, T.Yamagishi, H.Baba, Y.Honma, H.Tsukano, Y.Kubota, R.Hishida, K.Shibuki, S.Takahashi
  • Organizer: The 29^<th> Politzer Society Meeting
  • Place of Presentation: Susesi Resort - Antalya, Turkey
  • Year and Date: 2013-11-13
• [Presentation] Bilateral functional differences in the mouse auditory cortex using flaboprotein autofluoresence imaging (2013)
  • Author(s): K.Takahashi, H.Baba, Y.Honma, S.Ohshima, Y.Kubota, S.Takahashi
  • Organizer: 2^<nd> Meeting of European Academy of ORL-HNS
  • Place of Presentation: Nice, France
  • Year and Date: 2013-04-27
• [Presentation] マウス聴覚野OFF反応のイメージング解析 (2013)
  • Author(s): 馬場洋徳、塚野浩明、本間悠介、大島伸介、窪田和、高橋邦行、澁木克栄、髙橋姿
  • Organizer: 第11回側頭骨疾患研究会
  • Place of Presentation: 東京
  • Year and Date: 2013-01-12
• [Presentation] Bilateral functional differences in the mouse auditory cortex using flavoprotein autofluoresence imaging (2013)
  • Author(s): K.Takahashi, H.Baba, Y.Honma, S.Ohshima, Y.Kubota, S.Takahashi
  • Organizer: 2nd Meeting of European Academy of ORL-HNS
  • Place of Presentation: Nice, France
• [Presentation] Cortical depression in the mouse auditory cortex after sound discrimination learning (2013)
  • Author(s): S.Ohshima, K.Takahashi, T.Yamagishi, H.Baba, Y.Honma, H.Tsukano, Y.Kubota, R.Hishida, K.Shibuki, S.Takahashi
  • Organizer: The 29th Politzer Society Meeting
  • Place of Presentation: Antalya, Turkey
• [Presentation] 先行音持続刺激により出現するマウス聴覚野の二相性応答パターン (2012)
  • Author(s): 馬場洋徳、本間悠介、大島伸介、窪田和、高橋邦行、澁木克栄、髙橋姿
  • Organizer: 第57回日本聴覚医学会総会・学術講演会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2012-10-12
• [Presentation] マウス聴覚野における二相性ON-OFF応答の持続刺激後の出現 (2012)
  • Author(s): 馬場洋徳、塚野浩明、本間悠介、大島伸介、窪田和、高橋邦行、菱田竜一、髙橋姿、澁木克栄
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-21
• [Presentation] マウス大脳聴覚野の周波数変調(FM)音処理機構 (2012)
  • Author(s): 本間悠介、高橋邦行、塚野浩明、堀江正男、馬場洋徳、大島伸介、窪田和、澁木克栄、髙橋姿
  • Organizer: 第113回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 新潟,朱鷺メッセ
  • Year and Date: 2012-05-10
• [Presentation] ON and OFF responses in the mouse auditory cortex after exposure to long-lasting tone bursts (2012)
  • Author(s): 馬場洋徳、塚野浩明、本間悠介、大島伸介、窪田和、高橋邦行、菱田竜一、髙橋姿、澁木克栄
  • Organizer: 第89回日本生理学大会
  • Place of Presentation: 長野県松本文化会館
  • Year and Date: 2012-03-30
• [Presentation] マウス大脳聴覚野におけるFM音情報処理領域 (2012)
  • Author(s): 本間悠介, 高橋邦行, 塚野浩明, 堀江正男, 馬場洋徳, 大島伸介, 窪田和, 澁木克栄, 高橋姿
  • Organizer: 第10回側頭骨疾患研究会
  • Place of Presentation: 仙台
• [Presentation] ON and OFF responses in the mouse auditory cortex after exposure to long-lasting tone bursts (2012)
  • Author(s): 馬場洋徳, 塚野浩明, 本間悠介, 大島伸介, 窪田和, 高橋邦行, 菱田竜一, 高橋姿, 澁木克栄
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
• [Presentation] ON and OFF responses in the mouse auditory cortex disclosed after sound exposure (2011)
  • Author(s): 馬場洋徳、塚野浩明、本間悠介、大島伸介、窪田和、高橋邦行、菱田竜一、髙橋姿、澁木克栄
  • Organizer: 第16回聴覚研究フォーラム
  • Place of Presentation: 同支社びわこリトリートセンター
  • Year and Date: 2011-12-03
• [Presentation] ON and OFF responses in the mouse auditory cortex disclosed after sound exposure (2011)
  • Author(s): 馬場洋徳, 塚野浩明, 本間悠介, 大島伸介, 窪田和, 高橋邦行, 菱田竜一, 高橋姿, 澁木克栄
  • Organizer: 第16回聴覚研究フォーラム
  • Place of Presentation: 滋賀
• [Presentation] 先行持続音刺激により出現するマウス聴覚野の二相性応答パターン
  • Author(s): 馬場洋徳、本間悠介、大島伸介、窪田和、高橋邦行、澁木克栄、髙橋姿
  • Organizer: 第57回日本聴覚医学会総会・学術講演会
  • Place of Presentation: 国立京都国際会館
• [Presentation] マウス聴覚野における二相性ON-OFF応答の持続音刺激後の出現
  • Author(s): 馬場洋徳、塚野浩明、本間悠介、大島伸介、窪田和、高橋邦行、菱田竜一、髙橋姿、澁木克栄
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] マウス大脳聴覚野の周波数変調（FM）音処理機構
  • Author(s): 本間悠介、高橋邦行、塚野浩明、堀江正男、馬場洋徳、大島伸介、窪田和、澁木克栄、髙橋姿
  • Organizer: 第113回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 新潟朱鷺メッセ